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Invitrogen
RPA70 Monoclonal Antibody (11H4)
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图: 1 / 6
RPA70 Antibody (MA5-36226) in ICC/IF
Immunocytochemistry analysis of RPA70 in A549 cells. Antigen retrieval was performed with enzyme antigen retrieval (15 min). Samples were blocked with 10% goat serum and incubated in RPA70 monoclonal antibody (Product # MA5-36226) at a dilution of 2 µg/mL (overnight, 4°C), followed by Dylight 488 conjugated goat anti-mouse IgG (30 min, 37°C) and DAPI at a dilution of 5-10 µg/1x10^6 cells (30 min, 20°C).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
MA5-36226
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Non-human primate
宿主/亚型
Mouse
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
11H4
抗原
A synthetic peptide corresponding to a sequence at the C-terminus of human RPA70 (533-568aa QESAEAILGQNAAYLGELKDKNEQAFEEVFQNANFR).
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
浓度
500 µg/mL
规格
100 µg
纯化类型
Affinity chromatography
保存液
PBS with 4mg trehalose
内含物
0.05mg sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: HELA whole cell, MCF-7 whole cell, K562 whole cell, COS-7 whole cell, Caco-2 whole cell, A549 whole cell, HEPG2 whole cell, PC-3 whole cell, HEPA1-6 whole cell. IHC: human intestinal cancer tissue, human lung cancer tissue, human lung cancer tissue. ICC/IF: A549 cell. Flow: A431 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
This gene Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Replication factor A protein 1; Replication protein A 70 kDa DNA-binding subunit; RF-A protein 1; RP-A p70; Single-stranded DNA-binding protein
基因别名: 5031405K23Rik; 70kDa; AA589576; AW557552; REPA1; RF-A; RP-A; Rpa; RPA1; RPA70
UniProt ID: (Mouse) Q8VEE4
Entrez Gene ID: (Mouse) 68275
cell cycle checkpoint
telomere maintenance
double-strand break repair via homologous recombination
in utero embryonic development
DNA replication
DNA repair
base-excision repair
nucleotide-excision repair
mismatch repair
DNA recombination
cellular response to DNA damage stimulus
meiotic nuclear division
positive regulation of cell proliferation
hemopoiesis
homeostasis of number of cells within a tissue
chromosome organization
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Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
