Invitrogen

COPE Monoclonal Antibody (9B6)

Name: COPE Monoclonal Antibody (9B6)
Brand: Invitrogen
SKU: MA5-36232
Availability: InStock

查看其他7个COPE抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (10)

COPE Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 10

COPE Antibody (MA5-36232) in ICC/IF

Immunocytochemistry analysis of COPE using anti-COPE antibody (Product # MA5-36232). COPE was detected in a section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 5μg/mL mouse anti-COPE antibody (Product # MA5-36232) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualiz... View More

COPE Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

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Immunogen image

Immunogen Image COPE Monoclonal Antibody (9B6)

产品信息

MA5-36232

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

免疫细胞化学 (ICC/IF)

5 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

9B6

抗原

E. coli-derived human COPE recombinant protein (Position: E80-A308).

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

100 µg

纯化类型

Affinity chromatography

保存液

PBS with 4mg trehalose

内含物

0.05mg sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2884066

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human HEK293 whole cell, human HepG2 whole cell, human placenta tissue, human Caco-2 whole cell, human A549 whole cell, human PANC-1 whole cell, human SW579 whole cell. IHC: human mammary cancer tissue, human tonsil tissue, human intestinal cancer tissue, human lung cancer tissue, mouse intestine tissue, rat intestine tissue. ICC/IF: A431 cell. Flow: A431 cell, HepG2 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

靶标信息

The product of this gene is an epsilon subunit of coatomer protein complex. Coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles. It is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. Coatomer complex consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Alternatively spliced transcript variants encoding different isoforms have been identified.

仅用于科研。不用于诊断过程。未经明确授权不得转售。