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Invitrogen
DKK1 Recombinant Rabbit Monoclonal Antibody (1D12)
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FIGURE: 1 / 2
DKK1 Antibody (MA5-38364) in WB
Western blot was performed using Anti-DKK1 Recombinant Rabbit Monoclonal Antibody (1D12) (Product # MA5-38364) and a 28-40 kDa band corresponding to DKK1 was observed in HCT 116 treated with Protein transport inhibitor (1X for 4hrs) but not in U-937 cells which is reported to low or negative for DKK1 expression. Additional uncharacterized bands (*) were observed accross the cell lines. Whole cell extracts (30 µg lysate) of HCT 116 (Lane 1), HCT 116 treated with Protein transport inhibitor (1x for 4hrs) (Lane 2), U-937 (Lane 3), U-937 treat... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-38364
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
1D12
Immunogen
A synthesized peptide derived from human DKK1
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.2 mg/mL
Amount
120 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol, 150mM NaCl
Contains
0.02% sodium azide
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody has been tested in direct-ELISA
Target Information
DKK-1 is a member of the DKK protein family which also includes DKK-2, DKK-3 and DKK-4. DKK-1 was originally identified as a Xenopus head-forming molecule that behaves as an antagonist for Wnt signaling. Subsequent studies have shown that DKK-1 and DKK-4 play important regulatory roles in the Wnt/beta-catenin signaling pathway by forming inhibitory complexes with LDL receptor-related proteins 5 and 6 (LRP5 and LRP6), which are essential components of the Wnt/beta-catenin signaling system. LRP5 and LRP6 are single-pass transmembrane proteins that appear to act as co-receptors for Wnt ligands involved in the Wnt/beta-catenin signaling cascade. It has been suggested that by inhibiting Wnt/beta-catenin signaling, which is essential for posterior patterning in vertebrates, DKK-1 permits anterior development. This notion is supported by the finding that mice deficient of DKK-1 expression lack head formation and die during embryogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: dickkopf 1 homolog; dickkopf-1 like; dickkopf-like protein 1; Dickkopf1; hDkk1; secreted wnt inhibitor with affinity for heparin sepharose; unnamed protein product
Gene Aliases: DKK-1; SK
UniProt ID: (Human) O94907
Entrez Gene ID: (Human) 22943
negative regulation of transcription from RNA polymerase II promoter
regulation of receptor internalization
heart induction
heart valve development
endocardial cushion development
signal transduction
learning or memory
positive regulation of gene expression
negative regulation of neuron projection development
negative regulation of Wnt signaling pathway
negative regulation of ossification
negative regulation of mesodermal cell fate specification
regulation of endodermal cell fate specification
negative regulation of apoptotic process
regulation of neuron apoptotic process
positive regulation of Wnt signaling pathway, calcium modulating pathway
positive regulation of JNK cascade
regulation of synapse organization
regulation of synaptic transmission, glutamatergic
limb development
negative regulation of SMAD protein import into nucleus
motor learning
negative regulation of canonical Wnt signaling pathway
synapse pruning
regulation of dopaminergic neuron differentiation
negative regulation of Wnt-Frizzled-LRP5/6 complex assembly
positive regulation of midbrain dopaminergic neuron differentiation
negative regulation of presynapse assembly
positive regulation of Wnt signaling pathway, planar cell polarity pathway
negative regulation of cardiac muscle cell differentiation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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