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Invitrogen
PIM2 Recombinant Rabbit Monoclonal Antibody (JE55-57)
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图: 1 / 9
PIM2 Antibody (MA5-42509) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of of PIM2 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with PIM2 Recombinant Monoclonal Antibody (Product # MA5-42509) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
MA5-42509
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
JE55-57
抗原
Synthetic peptide within C-terminal Human PIM2.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
Protein A
保存液
TBS, pH 7.4, with 40% glycerol, 0.05% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Positive Control: Rat liver tissue lysate, mouse liver tissue lysate, human small intestine tissue lysate, 293T, A431, rat testis tissue, human liver tissue, human liver carcinoma tissue, human colon carcinoma tissue, mouse small intestine tissue, HepG2.
Subcellular Location: Cytoplasm.
靶标信息
PIM2 is a proto-oncogene which phosphorylates BAD, which partially inhibits its apopotopic function. PIM proteins (PIM-1, PIM-2 and PIM-3) are oncogene-encoded serine/threonine kinases. PIM-2 is highly homologous to Pim-1 with similar oncogenic functions. PIM-2 is most abundantly expressed in hematopoietic tissues, spleen, thymus, and peripheral blood leukocytes, as well as in testis, small intestine, and colon. PIM2 expression is negatively regulated by growth factor depletion. Increased levels of PIM-2 has also been observed in certain cancers.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: pim-2 oncogene; Pim-2h; Serine/threonine-protein kinase pim-2
基因别名: DXCch3; Pim-2; PIM2
UniProt ID: (Mouse) Q62070
Entrez Gene ID: (Rat) 317366, (Mouse) 18715
G1/S transition of mitotic cell cycle
protein phosphorylation
apoptotic process
cell cycle
regulation of mitotic cell cycle
negative regulation of cell proliferation
apoptotic mitochondrial changes
positive regulation of autophagy
phosphorylation
negative regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of transcription, DNA-templated
protein autophosphorylation
protein stabilization
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Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
