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Invitrogen
NSUN4 Monoclonal Antibody (4G4)
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NSUN4 Antibody (MA5-42532) in IHC (P)
Immunohistochemistry (Paraffin) analysis of paraffin-embedded human prostate tissue using NSUN4 Monoclonal Antibody (Product # MA5-42532). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the NSUN4 antibody at a dilution o... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
MA5-42532
产品规格
种属反应
Human,
Mouse
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
4G4
抗原
Recombinant protein within human NSUN4 aa 1-384.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
2 mg/mL
规格
200 µg
纯化类型
Protein G
保存液
TBS, pH 7.4, with 50% glycerol, 0.2% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Positive Control: MCF-7 cell lysate, 293 cell lysate, mouse testis tissue, human prostate tissue.
Subcellular Location: Mitochondrion.
靶标信息
NSUN4 is a 5-methylcytosine rRNA methytransferase that is involved in mitochondiral ribosome assembly. More specifically NSUN4 is involved in mitochondrial ribosome small subunit (SSU) maturation by methylation of mitochondrial 12S rRNA. This function is independent of MTREFD2/MTERF4 and assembled mitochondrial ribosome large subunit (LSU). NSUN4 is targeted to LSU by MTREFD2/MTERF4 and is involved in the final step in ribosome biogenesis to ensure that SSU and LSU are assembled.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 5-cytosine rRNA methyltransferase NSUN4; 5-cytosine tRNA methyltransferase NSUN4; mRNA cytosine C(5)-methyltransferase NSUN4; NOL1/NOP2/Sun domain family member 4; putative methyltransferase NSUN4; RP4-603I14.2; Sperm head and tail associated protein
基因别名: 2310010O12Rik; 2810405F18Rik; AI507900; NSUN4; Shtap
UniProt ID: (Mouse) Q9CZ57
Entrez Gene ID: (Mouse) 72181
rRNA (adenine-N6,N6-)-dimethyltransferase activity
RNA binding
protein C-terminal carboxyl O-methyltransferase activity
tRNA (guanine-N2-)-methyltransferase activity
methyltransferase activity
C-methyltransferase activity
N-methyltransferase activity
O-methyltransferase activity
S-methyltransferase activity
RNA methyltransferase activity
mRNA methyltransferase activity
tRNA methyltransferase activity
protein methyltransferase activity
2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity
rRNA methyltransferase activity
rRNA (uridine-2'-O-)-methyltransferase activity
rRNA (adenine-N6-)-methyltransferase activity
DNA-methyltransferase activity
tRNA (guanine-N1-)-methyltransferase activity
rRNA (cytosine-C5-)-methyltransferase activity
selenocysteine methyltransferase activity
arginine N-methyltransferase activity
protein-arginine N-methyltransferase activity
lysine N-methyltransferase activity
protein-lysine N-methyltransferase activity
tRNA (uracil) methyltransferase activity
tRNA (guanine) methyltransferase activity
tRNA (adenine) methyltransferase activity
tRNA (cytosine) methyltransferase activity
tRNA (cytosine-5-)-methyltransferase activity
tRNA (adenine-N1-)-methyltransferase activity
rRNA (adenine) methyltransferase activity
rRNA (cytosine) methyltransferase activity
rRNA (guanine) methyltransferase activity
rRNA (uridine) methyltransferase activity
transferase activity
protein C-terminal leucine carboxyl O-methyltransferase activity
1-phenanthrol methyltransferase activity
protein-arginine N5-methyltransferase activity
rRNA binding
methylarsonite methyltransferase activity
dimethylarsinite methyltransferase activity
4,5-dihydroxybenzo(a)pyrene methyltransferase activity
1-hydroxypyrene methyltransferase activity
1-hydroxy-6-methoxypyrene methyltransferase activity
demethylmenaquinone methyltransferase activity
cobalt-precorrin-6B C5-methyltransferase activity
cobalt-precorrin-7 C15-methyltransferase activity
cobalt-precorrin-5B C1-methyltransferase activity
cobalt-precorrin-3 C17-methyltransferase activity
dimethylamine methyltransferase activity
hydroxyneurosporene-O-methyltransferase activity
tRNA (adenine-57, 58-N(1)-) methyltransferase activity
methylamine-specific methylcobalamin:coenzyme M methyltransferase activity
trimethylamine methyltransferase activity
methanol-specific methylcobalamin:coenzyme M methyltransferase activity
monomethylamine methyltransferase activity
P-methyltransferase activity
Se-methyltransferase activity
2-phytyl-1,4-naphthoquinone methyltransferase activity
tRNA (uracil-2'-O-)-methyltransferase activity
tRNA (cytosine-2'-O-)-methyltransferase activity
phosphomethylethanolamine N-methyltransferase activity
tRNA (cytosine-3-)-methyltransferase activity
small ribosomal subunit rRNA binding
rRNA (cytosine-2'-O-)-methyltransferase activity
rRNA (cytosine-N4-)-methyltransferase activity
trihydroxyferuloyl spermidine O-methyltransferase activity
RNA methyltransferase
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