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Invitrogen
Cofilin Recombinant Rabbit Monoclonal Antibody (4M1Z9)
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FIGURE: 1 / 1
Cofilin Antibody (MA5-42790) in WB
Western blot analysis of Cofilin in extracts of various cell lines. Samples were incubated with Cofilin Monoclonal antibody (Product # MA5-42790) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-42790
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
4M1Z9
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Cofilin (P23528).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.5 mg/mL
Amount
150 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive test controls include: HeLa, 293T, MCF7, NIH/3T3, C6, Mouse lung, Mouse thymus, Rat brain. The target is usually found in the following locations: Cell projection, Cytoplasm, Cytoplasmic side, Nucleus matrix, Peripheral membrane protein, cytoskeleton, lamellipodium membrane, ruffle membrane.
Immunogen sequence: MASGVAVSDG VIKVFNDMKV RKSSTPEEVK KRKKAVLFCL SEDKKNIILE EGKEILVGDV GQTVDDPYAT FVKMLPDKDC RYALYDATYE TKESKKEDLV
Target Information
Cofilin binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. Required for neural tube morphogenesis and neural crest cell migration.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 18 kDa phosphoprotein; CFL-1; COF1; Cofilin, non-muscle isoform; Cofilin-1; Cofilin1; p18
Gene Aliases: AA959946; CFL; CFL1; Cof; n-cofilin
UniProt ID: (Rat) P45592, (Mouse) P18760
Entrez Gene ID: (Rat) 29271, (Mouse) 12631
mitotic cytokinesis
neural crest cell migration
neural fold formation
protein phosphorylation
protein import into nucleus
cytoskeleton organization
actin filament organization
response to virus
regulation of cell morphogenesis
establishment of cell polarity
cell projection organization
actin filament depolymerization
positive regulation of actin filament depolymerization
response to amino acid
negative regulation of cell size
regulation of dendritic spine morphogenesis
movement of cell or subcellular component
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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