Invitrogen

HDAC1 Monoclonal Antibody (E22)

查看其他39个HDAC1抗体

问题与答案

问题与答案

问题与答案

Additional Information:

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抗体检测数据 (8)

HDAC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HDAC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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HDAC1 Antibody (MA5-43780) in IHC (P)

Immunohistochemistry (Paraffin) analysis of HDAC1 in human lung cancer tissue using HDAC1 Monoclonal Antibody (E22) (Product # MA5-43779) (10 µg/mL). The sample was then stained with HRP-Goat anti-Mouse IgG polyclonal secondary antibody (2 µg/mL). DAB staining.

HDAC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HDAC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HDAC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HDAC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HDAC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HDAC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HDAC1 Antibody in Western Blot (WB)

HDAC1 Antibody in Flow Cytometry (Flow)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

HDAC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of HDAC1 in human lung cancer tissue using HDAC1 Monoclonal Antibody (E22) (Product # MA5-43779) (10 µg/mL). The sample was then stained with HRP-Goat anti-Mouse IgG polyclonal secondary antibody (2 µg/mL). DAB staining.

HDAC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of HDAC1 in mouse cerebellum tissue using HDAC1 Monoclonal Antibody (E22) (Product # MA5-43779) (10 µg/mL). The sample was then stained with HRP-Goat anti-Mouse IgG polyclonal secondary antibody (2 µg/mL). DAB staining.

HDAC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of HDAC1 in mouse pancreas tissue using HDAC1 Monoclonal Antibody (E22) (Product # MA5-43779) (10 µg/mL). The sample was then stained with HRP-Goat anti-Mouse IgG polyclonal secondary antibody (2 µg/mL). DAB staining.

HDAC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of HDAC1 in human placenta tissue using HDAC1 Monoclonal Antibody (E22) (Product # MA5-43779) (10 µg/mL). The sample was then stained with HRP-Goat anti-Mouse IgG polyclonal secondary antibody (2 µg/mL). DAB staining.

HDAC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of HDAC1 in rat testis tissue using HDAC1 Monoclonal Antibody (E22) (Product # MA5-43779) (10 µg/mL). The sample was then stained with HRP-Goat anti-Mouse IgG polyclonal secondary antibody (2 µg/mL). DAB staining.

HDAC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of HDAC1 in rat colon tissue using HDAC1 Monoclonal Antibody (E22) (Product # MA5-43779) (10 µg/mL). The sample was then stained with HRP-Goat anti-Mouse IgG polyclonal secondary antibody (2 µg/mL). DAB staining.

HDAC1 Antibody in Western Blot (WB) — Western blot analysis of HDAC1 using HDAC1 Monoclonal Antibody (E22) (Product # MA5-43779) at a dilution of 0.2 µg/mL. Lane 1: Human Lung lysate; Lane 2: A549 cell lysate; Lane 3: HepG2 cell lysate; Lane 4: Bovine Testis lysate; Lane 5: Rat Testis lysate . Secondary antibody used is HRP-Rabbit anti-Mouse IgG polyclonal antibody at a dilution of 0.2 µg/mL.

HDAC1 Antibody in Flow Cytometry (Flow) — Flow cytometry analysis of HDAC1 in Human Jurkat using HDAC1 Monoclonal Antibody (E22) (Product # MA5-43779). Cells were fixed with 2% paraformaldehyde (10 min), permeabilised with 0.2% BSA-Triton X-100, then stained with 20 µg/mL HDAC1 Monoclonal Antibody (E22) (red histogram) or Isotype control antibody (green histogram) followed by 1 µg/mL FITC-conjugated anti-mouse IgG secondary antibody.

Immunogen image

HDAC1 Monoclonal Antibody (E22)

Immunogen Image HDAC1 Monoclonal Antibody (E22)

产品信息

MA5-43780

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.01-2 µg/mL

-

免疫组化（石蜡） (IHC (P))

5-20 µg/mL

-

免疫细胞化学 (ICC/IF)

5-20 µg/mL

-

流式细胞分析 (Flow)

10 µg/mL

-

产品规格

种属反应

Bovine, Human, Mouse, Rat

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

E22

抗原

Met1-Ala482 (Accession Q13547), with N-terminal His Tag

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

20 µg

纯化类型

Protein A/G

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.05% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. HDAC1 belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, HDAC1 deacetylates p53 and modulates its effect on cell growth and apoptosis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: DKFZp686H12203; HD1; Histone deacetylase 1; Protein deacetylase HDAC1; Protein deacylase HDAC1; RP4-811H24.2

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基因别名: HD1; HDAC1; Hdac1-ps; MommeD5; RPD3; RPD3L1

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UniProt ID: (Bovine) Q32PJ8, (Mouse) O09106, (Rat) Q4QQW4

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Entrez Gene ID: (Bovine) 404126, (Mouse) 433759, (Rat) 297893

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histone deacetylase activity deacetylase activity NAD-dependent histone deacetylase activity (H3-K14 specific) transcription regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding core promoter binding RNA polymerase II repressing transcription factor binding RNA polymerase II transcription corepressor activity DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription corepressor activity protein binding transcription factor binding hydrolase activity enzyme binding histone deacetylase activity (H3-K14 specific) nucleosomal DNA binding histone deacetylase activity (H3-K9 specific) protein complex binding protein deacetylase activity activating transcription factor binding histone deacetylase activity (H4-K16 specific) Krueppel-associated box domain binding histone deacetylase binding transcription regulatory region DNA binding NAD-dependent histone deacetylase activity (H3-K9 specific) NAD-dependent histone deacetylase activity (H4-K16 specific) protein N-terminus binding NF-kappaB binding repressing transcription factor binding NAD-dependent histone deacetylase activity (H3-K18 specific)

circadian regulation of gene expression negative regulation of transcription, DNA-templated histone H4 deacetylation negative regulation of transcription from RNA polymerase II promoter response to amphetamine transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter protein deacetylation endoderm development circadian rhythm positive regulation of cell proliferation negative regulation of cell proliferation epidermal cell differentiation negative regulation of gene expression positive regulation of receptor biosynthetic process chromatin modification histone deacetylation hippocampus development neuron differentiation positive regulation of interleukin-1 production positive regulation of tumor necrosis factor production cellular response to oxidative stress odontogenesis of dentin-containing tooth positive regulation of tyrosine phosphorylation of Stat3 protein embryonic digit morphogenesis ATP-dependent chromatin remodeling negative regulation of apoptotic process negative regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of neuron apoptotic process negative regulation by host of viral transcription positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of insulin secretion rhythmic process positive regulation of oligodendrocyte differentiation negative regulation of androgen receptor signaling pathway hair follicle placode formation eyelid development in camera-type eye fungiform papilla formation histone H3 deacetylation negative regulation of canonical Wnt signaling pathway positive regulation of chemokine (C-X-C motif) ligand 2 production positive regulation of type B pancreatic cell apoptotic process negative regulation of peptidyl-lysine acetylation negative regulation of intrinsic apoptotic signaling pathway negative regulation of myotube differentiation response to caffeine response to lipopolysaccharide response to drug response to hyperoxia cellular response to tumor necrosis factor