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Invitrogen
PU.1 Recombinant Rabbit Monoclonal Antibody (PD00-14)
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FIGURE: 1 / 2
PU.1 Antibody (MA5-44648) in IHC (P)
Immunohistochemistry analysis of PU.1 in paraffin-embedded human B-cell lymphoma tissue. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with PU.1 Monoclonal antibody (Product # MA5-44648) using a dilution of 1:4,000 for 1 hour... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-44648
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
PD00-14
Immunogen
Synthetic peptide within Human PU.1 aa 1-100.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 31 kDa transforming protein; 31kDa Transforming Protein; hCG_25181; hematopoietic transcription factor PU.1; SPIA; spleen focus forming virus (SFFV) proviral integration oncogene spi1; Transcription Factor spi1; unnamed protein product
Gene Aliases: AGM10; OF; PU.1; SFPI1; SPI-1; SPI-A
UniProt ID: (Human) P17947
Entrez Gene ID: (Human) 6688
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
core promoter proximal region sequence-specific DNA binding
bacterial-type RNA polymerase transcriptional activator activity, sequence-specific DNA binding
bacterial-type RNA polymerase transcriptional repressor activity, sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
histone deacetylase binding
sequence-specific DNA binding
NFAT protein binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
STAT family protein binding
DNA-binding transcription factor binding
protein sequestering activity
negative regulation of transcription from RNA polymerase II promoter
germinal center B cell differentiation
follicular B cell differentiation
immature B cell differentiation
defense response to tumor cell
pro-T cell differentiation
myeloid leukocyte differentiation
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transforming growth factor beta receptor signaling pathway
positive regulation of gene expression
negative regulation of gene expression
cell differentiation
osteoclast differentiation
lipopolysaccharide-mediated signaling pathway
TRAIL-activated apoptotic signaling pathway
negative regulation of I-kappaB kinase/NF-kappaB signaling
negative regulation of neutrophil degranulation
negative regulation of MHC class II biosynthetic process
response to ethanol
positive regulation of B cell differentiation
regulation of erythrocyte differentiation
positive regulation of gene expression, epigenetic
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
interleukin-6-mediated signaling pathway
cellular response to ethanol
oncogene-induced cell senescence
endothelial to hematopoietic transition
negative regulation of protein localization to chromatin
positive regulation of p38MAPK cascade
negative regulation of NIK/NF-kappaB signaling
positive regulation of pri-miRNA transcription from RNA polymerase II promoter
positive regulation of microglial cell mediated cytotoxicity
negative regulation of adipose tissue development
pericyte cell differentiation
positive regulation of antifungal innate immune response
regulation of myeloid progenitor cell differentiation
positive regulation of myeloid dendritic cell chemotaxis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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