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Invitrogen
N-cadherin Recombinant Rabbit Monoclonal Antibody (020), PE
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N-cadherin Antibody (MA5-46720) in Flow
Flow cytometry analysis of N-cadherin in on HeLa cells using N-cadherin, PE Recombinant Monoclonal (Product # MA5-46720). HeLa cells were harvested with (Left) or without (Right) trypsinization (please note, the epitope is sensitive to trypsin) and stained with either Rabbit IgG isotype control FITC (dashed line) or N-cadherin, PE (solid line) at matching concentrations.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
MA5-46720
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
020
抗原
Recombinant protein ecoding Human N-cadherin (Met1-Ala724).
偶联物
PE
PE
PE
激发/发射光谱
565/576 nm
查看光谱
形式
Liquid
浓度
100 µg/mL
纯化类型
Protein A
保存液
PBS with 0.5% BSA
内含物
0.09% sodium azide
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human N-cadherin (Met1-Ala724) and conjugated with PE under optimum conditions, the unreacted PE was removed.
Storage: This antibody can be stored at 2C-8C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
靶标信息
N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. N-cadherin's function is dependent on its association with the actin-cytoskeleton and is mediated through interactions between the C-terminal region of N-cadherin and the cytoplasmic catenin proteins. The stability of this association is regulated by phosphorylation and dephosphorylation of beta-catenin. Further, N-cadherin is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Cadherin-2; Cadherin-N; Cadherin2; CD325; CDw325; CDw325 antigen; N-cadherin; Neural cadherin
基因别名: CDH2; CDHN; NCAD
Entrez Gene ID: (Human) 1000
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