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Invitrogen
CD100 Recombinant Rabbit Monoclonal Antibody (010), APC
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-46845
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
010
Immunogen
Recombinant protein encoding Mouse CD100 (Met1-Arg733).
Conjugate
APC
APC
APC
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
100 µg/mL
Purification
Protein A
Storage buffer
PBS with 0.5% BSA
Contains
0.03% ProClin 300
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD100 (SEMA4D) (Met1-Arg733) and conjugated with APC under optimum conditions, the unreacted APC was removed.
Storage: This antibody can be stored at 2C-8C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Target Information
Semaphorins are neuronal chemorepellants that direct pioneering neurons during nervous system development. CD100 (also known as semaphorin 4d), a 150 kDa homodimer, is a novel semaphorin that induces B cells to aggregate and improves their viability in vitro. CD100 modifies CD40-CD40L B cell signaling by augmenting B cell aggregation and survival and down regulating CD23 expression. CD72 is a lymphocyte receptor for the class IV semaphorin CD100, which is involved in regulating B cell signaling. CD100 is expressed on resting and PHA stimulated T cells. The protein is weakly expressed on NK cells, EBV transformed B cells, monocytes and tumor/peripheral blood T cell lines, and at higher density on activated T cells and B cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD100; M-Sema G; Sema J; semaphorin H; Semaphorin-4D; Semaphorin-C-like 2; Semaphorin-J
Gene Aliases: CD100; coll-4; Sema4d; Semacl2; Semaj; Semcl2
UniProt ID: (Mouse) O09126
Entrez Gene ID: (Mouse) 20354
negative regulation of transcription from RNA polymerase II promoter
positive regulation of protein phosphorylation
negative regulation of cell adhesion
multicellular organism development
nervous system development
regulation of cell shape
negative regulation of alkaline phosphatase activity
positive regulation of phosphatidylinositol 3-kinase signaling
cell differentiation
positive regulation of cell migration
regulation of cell projection organization
positive regulation of GTPase activity
ossification involved in bone maturation
negative regulation of osteoblast differentiation
positive regulation of collateral sprouting
regulation of dendrite morphogenesis
positive regulation of peptidyl-tyrosine phosphorylation
negative regulation of peptidyl-tyrosine phosphorylation
positive regulation of axonogenesis
leukocyte aggregation
semaphorin-plexin signaling pathway
semaphorin-plexin signaling pathway involved in bone trabecula morphogenesis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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