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Invitrogen
Optineurin Monoclonal Antibody (3D8)
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FIGURE: 1 / 1
Optineurin Antibody (MA5-49177) in WB
Western blot analysis of Optineurin/OPTN using Optineurin/OPTN antibody (Product # MA5-49177). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 µg of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates; Lane 2: human U20S whole cell lysates; Lane 3: human placenta tissue lysates; Lane 4: human Hela whole cell lysates; Lane 5: human A549 whole cell lysates; Lane 6: human K562 whole cell lysates; Lane 7: rat C... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-49177
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
3D8
Immunogen
E.coli-derived human Optineurin recombinant protein (Position: R241-I577).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Human Optineurin shares 82% amino acid (aa) sequence identity with both mouse and rat Optineurin.
Positive Control - WB: human U-87MG whole cell, human U20S whole cell, human placenta tissue, human Hela whole cell, human A549 whole cell, human K562 whole cell, rat C6 whole cell, mouse Neuro-2a whole cell. Flow: U20S cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
This gene encodes the coiled-coil containing protein optineurin. Optineurin may play a role in normal-tension glaucoma and adult-onset primary open angle glaucoma. Optineurin interacts with adenovirus E3-14. 7K protein and may utilize tumor necrosis factor-alpha or Fas-ligand pathways to mediate apoptosis, inflammation or vasoconstriction. Optineurin may also function in cellular morphogenesis and membrane trafficking, vesicle trafficking, and transcription activation through its interactions with the RAB8, huntingtin, and transcription factor IIIA proteins. Alternative splicing results in multiple transcript variants encoding the same protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 14.7K-interacting protein 2; Ag9 C5; E3-14.7K-interacting protein; FIP 2; FIP-2; FIP-2-like protein; HIP 7; HIP-7; Huntingtin yeast partner L; Huntingtin-interacting protein 7; Huntingtin-interacting protein L; NEMO-related protein; Optic neuropathy-inducing protein; Optineurin; TFIIIA IntP; TFIIIA-INTP; Transcription factor IIIA-interacting protein; Uban
Gene Aliases: 4930441O07Rik; FIP2; GLC1E; HIP7; HYPL; NRP; OPTN
UniProt ID: (Rat) Q8R5M4, (Mouse) Q8K3K8
Entrez Gene ID: (Rat) 246294, (Mouse) 71648
protein targeting to Golgi
negative regulation of receptor recycling
autophagy
Golgi organization
positive regulation of cell proliferation
macroautophagy
cellular protein localization
wound healing
Golgi to plasma membrane protein transport
regulation of I-kappaB kinase/NF-kappaB signaling
negative regulation of I-kappaB kinase/NF-kappaB signaling
negative regulation of neuron apoptotic process
cytokine secretion
defense response to Gram-negative bacterium
protein homooligomerization
Golgi ribbon formation
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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