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          • Primary Antibodies ›
          • SND1 Antibodies

          Invitrogen

          SND1 Monoclonal Antibody (6G3B4)

          View all (13) SND1 antibodies

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          Cite SND1 Monoclonal Antibody (6G3B4)

          Additional Information:

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          • Antibody Testing Data (10)
          SND1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          SND1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 10

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          SND1 Antibody (MA5-49230) in ICC/IF

          Immunocytochemistry analysis of SND1 in PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in SND1 Monoclonal antibody (Product # MA5-49230) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for t... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          SND1 Antibody in Immunocytochemistry (ICC/IF)
          SND1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SND1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SND1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SND1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SND1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SND1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SND1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SND1 Antibody in Western Blot (WB)
          SND1 Antibody in Flow Cytometry (Flow)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          SND1 Monoclonal Antibody (6G3B4)

          Product Details

          MA5-49230

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.25-0.5 µg/mL
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          2-5 µg/mL
          -

          Immunocytochemistry (ICC/IF)

          5 µg/mL
          -

          Flow Cytometry (Flow)

          1-3 µg/1x10^6 cells
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Mouse / IgG2a

          Class

          Monoclonal

          Type

          Antibody

          Clone

          6G3B4

          Immunogen

          E.coli-derived human SND1 recombinant protein (Position: Q20-D204).
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Lyophilized

          Concentration

          500 µg/mL

          Amount

          100 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS with 4mg trehalose

          Contains

          no preservative

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_3074888

          Product Specific Information

          Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

          Positive Control - WB: human Hela whole cell, human Raji whole cell, human U87 whole cell, rat stomach tissue, rat pancrease tissue, mouse stomach tissue, mouse pancrease tissue. IHC: human liver cancer tissue, human metaplasia of squamous cells of the renal pelvis tissue, human tonsil tissue, human rectum adenocarcinoma tissue, mouse brain tissue, mouse brain tissue, rat brain tissue. ICC/IF: PC-3 cell. Flow: Hela cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

          Target Information

          SND1/P100 (staphylococcal nuclease and tudor domain containing 1), also known as TudorSN, functions in the Pim-1 regulation of Myb activity and acts as a transcriptional activator of EBNA-2. It also interacts with EAV, NSP1, GTF2E1 and GTF2E2, and forms a ternary complex with Stat6 and POLR2A. The staphylococcal nuclease-like (SN)-domains directly interact with amino acids 1099-1758 of CBP. SND1/P100 plays an important role in the assembly of Stat6 transcriptome and stimulates IL-4-dependent transcription by mediating interaction between Stat6 and CBP.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: 100 kDa coactivator; EBNA2 coactivator p100; p100 co-activator; p105 coactivator; staphylococcal nuclease domain containing 1; testis tissue sperm-binding protein Li 82P; tudor domain-containing protein 11; TudorSN; unnamed protein product

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          Gene Aliases: p100; SND p102; TDRD11; TSN; Tudor-SN

          View more View less

          UniProt ID: (Human) Q7KZF4, (Rat) Q66X93, (Mouse) Q78PY7

          View more View less

          Entrez Gene ID: (Human) 27044, (Rat) 64635, (Mouse) 56463

          View more View less

          Function(s)
          nucleic acid binding transcription cofactor activity RNA binding nuclease activity endonuclease activity endoribonuclease activity protein binding cadherin binding RISC complex binding 3' overhang single-stranded DNA endodeoxyribonuclease activity transcription coactivator activity
          Process(es)
          mRNA catabolic process regulation of cell cycle process miRNA catabolic process gene silencing by RNA negative regulation of apoptotic process positive regulation of transcription, DNA-templated osteoblast differentiation
          It has to be done as per old AB suggested Products section.

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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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