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          • Primary Antibodies ›
          • BRG1 Antibodies

          Invitrogen

          BRG1 Monoclonal Antibody (A5C9)

          View all (38) BRG1 antibodies

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          Cite BRG1 Monoclonal Antibody (A5C9)

          Additional Information:
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          • Antibody Testing Data (2)
          BRG1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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          BRG1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          BRG1 Antibody (MA5-50572) in IHC (P)

          Immunohistochemistry of BRG1 in paraffin-embedded human kidney tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with BRG1 monoclonal antibody (Product # MA5-50572) with a dilution of 1:100 for 30 minutes at room temperat... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          BRG1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          BRG1 Antibody in Western Blot (WB)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          BRG1 Monoclonal Antibody (A5C9)

          Product Details

          MA5-50572

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:200
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          A5C9

          Immunogen

          Synthetic peptide within human BRG1 aa 230-280.
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          2 mg/mL

          Amount

          200 µg

          Purification

          Protein G

          Storage buffer

          TBS/PBS, pH 7.4, with 0.05% BSA, 40% glycerol

          Contains

          0.05% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_3091237

          Product Specific Information

          Positive control: K562 cell lysate, 293T cell lysate, human kidney tissue.

          Predicted band size: 185 kDa

          Subcellular Location: Nucleus.

          Target Information

          Brg1 (Brahma-related gene 1) is an ATPase subunit of SWI2/SNF2-like chromatin-remodeling complexes that enable access of regulatory and effector proteins in transcription, DNA repair and DNA replication. Although Brg1-containing complexes are not essential for general cell survival, they participate in transcriptional regulation of several hundred genes including those involved in interferon and stress response, immune cells differentiation, neurogenesis, cell cycle etc. and is absolutely necessary for mouse embryogenesis. Brg1 is also involved in cell growth arrest, senescence and tumour supression.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: BAF190A; BRG1-associated factor 190A; FLJ39786; Mitotic growth and transcription activator; Protein brahma homolog 1; Protein BRG-1; SMARCA4; SNF2-beta; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4; Transcription activator BRG1

          View more View less

          Gene Aliases: BAF190A; BRG1; SMARCA4; SNF2B; SNF2L4

          View more View less

          Entrez Gene ID: (Human) 6597

          View more View less

          It has to be done as per old AB suggested Products section.

          Disclaimer

          Close

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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