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Invitrogen
CD68 Recombinant Mouse Monoclonal Antibody (PDM0-13)
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图: 1 / 8
CD68 Antibody (MA5-50651) in ICC/IF
Immunocytochemistry analysis of CD68 in THP-1 cells. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were incubated with CD68 recombinant monoclonal antibody (Product # MA5-50651) with a dilution of 1:100 in 1% BSA in PBST overnight at 4 &(8)451;. Followed by secondary antibody Goat Anti-Mouse IgG H&L (iFluor™ 488) at a dilution of 1:1,000. PBS i... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
MA5-50651
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
PDM0-13
抗原
Recombinant protein within Human CD68 aa 1-354.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
Protein A
保存液
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Positive control: Human spleen tissue, human diffuse large B-cell lymphoma tissue, human tonsil tissue, human liver tissue, THP-1, human non-small cell lung cancer, human cervical cancer.
Predicted band size: 37 kDa
Subcellular Location: Cell membrane Endosome membrane, Lysosome membrane.
靶标信息
CD68 (Macrosialin) is a 110 kDa integral membrane glycoprotein predominantly expressed on the intracellular lysomsomes of monocytes and macrophages and to a lesser extent by dendritic cells and peripheral blood granulocytes. Also, CD68 could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. CD68 is expressed by interdigitating reticulum cells in tonsil and some histiocytic lymphoma or histiocytosis, acute myeloid leukemia (AML), and granulocytic sarcoma. Elevated expression of CD68 has been demonstrated on CD34+ cells in various human malignancies, including several Acute Myeloid Leukemia studies.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
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