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Invitrogen
CD97 Recombinant Rabbit Monoclonal Antibody (3E5L8)
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FIGURE: 1 / 1
CD97 Antibody (MA5-51373) in WB
Western blot analysis of CD97 in U-937 cells. Samples were incubated with CD97 Recombinant monoclonal antibody (Product # MA5-51373) using a dilution of 1:2,000 followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:200,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-51373
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
3E5L8
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 21-398 of human CD97 (NP_510966.1).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.072 mg/mL
Amount
7.2 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: QDSRGCARWC PQNSSCVNAT ACRCNPGFSS FSEIITTPTE TCDDINECAT PSKVSCGKFS DCWNTEGSYD CVCSPGYEPV SGAKTFKNES ENTCQDVDEC QQNPRLCKSY GTCVNTLGSY TCQCLPGFKF IPEDPKVCTD VNECTSGQNP CHSSTHCLNN VGSYQCRCRP GWQPIPGSPN GPNNTVCEDV DECSSGQHQC DSSTVCFNTV GSYSCRCRPG WKPRHGIPNN QKDTVCEDMT FSTWTPPPGV HSQTLSRFFD KVQDLGRDSK TSSAEVTIQN VIKLVDELME APGDVEALAP PVRHLIATQL LSNLEDIMRI LAKSLPKGPF TYISPSNTEL TLMIQERGDK NVTMGQSSAR MKLNWAVAAG AEDPGPAV
Target Information
CD97 is a G-protein-coupled seven-span transmembrane adhesive receptor that is constitutively expressed on granulocytes and monocytes and rapidly upregulated on T and B cells upon activation. CD97 is produced in alternatively spliced forms and its cellular ligand is CD55 (DAF), which protects various cell types from complement-mediated damage. Interaction of CD97 on leukocytes and CD55 on vessel cells probably facilitate leukocyte activation and migration into the tissues, similarly, CD97 seems to play a role in tumour migration and invasiveness. CD97 is involved in T cell regulation and peripheral granulocyte homeostasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Adhesion G protein-coupled receptor E5; CD97; Leukocyte antigen CD97
Gene Aliases: ADGRE5; CD97
Entrez Gene ID: (Human) 976
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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