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Invitrogen
BRD9 Recombinant Rabbit Monoclonal Antibody (8C4G2)
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FIGURE: 1 / 1
BRD9 Antibody (MA5-51462) in WB
Western blot analysis of BRD9 in various lysates. Samples were incubated with BRD9 Recombinant monoclonal antibody (Product # MA5-51462) using a dilution of 1:2,000 followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-51462
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
8C4G2
Immunogen
Recombinant protein (or fragment)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.07 mg/mL
Amount
7 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: REVISCMFEP EGNACSLTDS TAEEHVLALV EHAADEARDR INRFLPGGKM GYLKRNGDGS LLYSVVNTAE PDADEEETHP VDLSSLSSKL LPGFTTLGFK DERRNKVTFL SSATTALSMQ NNSVFGDLKS DEMELLYSAY GDETGVQCAL SLQEFVKDAG SYSKKVVDDL LDQITGGDHS RTLFQLKQRR NVPMKPPDEA KVGDTLGDSS SSVLEFMSMK SYPDVSVDIS MLSSLGKVKK ELDPDDSHLN LDETTKLLQD LHEAQAERGG SRPSSNLSSL SNASERDQHH LGSPSRLSVG EQPDVTHDPY EFLQSPEPAA SAKT
Target Information
The BRD9 gene encodes a protein that is a crucial subunit of the SWI/SNF chromatin remodeling complex known as ncBAF (non-canonical BAF complex). As a bromodomain-containing protein, BRD9 acts as an 'epigenetic reader,' selectively recognizing acetyl-lysine marks on histones, which is essential for regulating chromatin structure and gene expression through the recruitment of acetylated transcriptional regulators. It has emerged as a significant player in epigenetic modulation and has been implicated in various cancers, such as cervical and non-small cell lung cancer, due to its over-expression. BRD9 is situated on the 5p arm of chromosome 5 and is a potential target for anticancer therapies, notably in acute myeloid leukemia (AML). Recent studies highlight the development of selective BRD9 inhibitors, indicating its potential for therapeutic targeting. Its role in cancer is linked to its function in transcriptional regulation and its involvement in the mammalian SWI/SNF complex, which is linked to tumor suppression mechanisms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp434D0711; DKFZp686L0539; FLJ43530; rhabdomyosarcoma antigen MU-RMS-40.8; sarcoma antigen NY-SAR-29; unnamed protein product
Gene Aliases: LAVS3040; PRO9856; SMARCI2
UniProt ID: (Human) Q9H8M2, (Mouse) Q3UQU0
Entrez Gene ID: (Human) 65980, (Rat) 308067, (Mouse) 105246
double-strand break repair via homologous recombination
chromatin organization
regulation of transcription from RNA polymerase II promoter
biological_process
chromatin remodeling
positive regulation of cell proliferation
negative regulation of cell differentiation
positive regulation of stem cell population maintenance
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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