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          • Primary Antibodies ›
          • GATA2/GATA3 Antibodies

          Invitrogen

          GATA2/GATA3 Recombinant Rabbit Monoclonal Antibody (23GB5730)

          Advanced Verification
          View all (6) GATA2/GATA3 antibodies

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          Cite GATA2/GATA3 Recombinant Rabbit Monoclonal Antibody (23GB5730)

          Additional Information:

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          • Antibody Testing Data (3)
          • Advanced Verification (1)
          GATA2/GATA3 Antibody in Immunocytochemistry (ICC/IF)
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          GATA2/GATA3 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 4

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          GATA2/GATA3 Antibody (MA5-53921) in ICC/IF

          Immunocytochemistry analysis of GATA2/GATA3 in HAP1 cells. Sample was incubated with GATA2/GATA3 monoclonal (Product # MA5-53920) antibody at a dilution of 1:1,000, followed by DAPI and Alexa Fluor 647. Protein abundance based on laser intensity and smart gain: Medium. Scale bar: 20 μm. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          View Product

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          GATA2/GATA3 Antibody in Immunocytochemistry (ICC/IF)
          GATA2/GATA3 Antibody in Western Blot (WB)
          GATA2/GATA3 Antibody in Flow Cytometry (Flow)
          GATA2/GATA3 Antibody

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          GATA2/GATA3 Recombinant Rabbit Monoclonal Antibody (23GB5730)

          Product Details

          MA5-53921

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000-1:5,000
          -

          Immunocytochemistry (ICC/IF)

          1:100-1:1,000
          -

          Flow Cytometry (Flow)

          1:200-1:2,000
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Expression System

          HEK293F

          Class

          Recombinant Monoclonal

          Type

          Antibody

          Clone

          23GB5730

          Immunogen

          A synthesized peptide derived from human GATA2/3 (1-250AA).
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.4, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_3248392

          Target Information

          The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells or macrophages. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises 6 exons distributed over 17 kb of DNA. Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: GATA-3; GATA-binding factor 3; GATA-bindning protein 2; MGC2346; MGC5199; MGC5445; unnamed protein product

          View more View less

          Gene Aliases: DCML; Gata-2; Gata-3; HDR; HDRS; IMD21; jal; MONOMAC; NFE1B

          View more View less

          UniProt ID: (Human) P23769, (Human) P23771, (Rat) Q924Y4, (Mouse) O09100, (Mouse) P23772

          View more View less

          Entrez Gene ID: (Human) 2624, (Human) 2625, (Rat) 25159, (Mouse) 14461, (Mouse) 14462, (Rat) 85471

          View more View less

          Function(s)
          RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription cofactor binding transcription coactivator binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding zinc ion binding sequence-specific DNA binding RNA polymerase II sequence-specific DNA binding transcription factor binding C2H2 zinc finger domain binding sequence-specific double-stranded DNA binding transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding interleukin-2 receptor binding identical protein binding E-box binding HMG box domain binding histone methyltransferase binding
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter urogenital system development cell fate determination neuron migration embryonic placenta development regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter positive regulation of cytosolic calcium ion concentration positive regulation of gene expression negative regulation of gene expression regulation of primitive erythrocyte differentiation ventral spinal cord interneuron differentiation cell differentiation in hindbrain commitment of neuronal cell to specific neuron type in forebrain central nervous system neuron development pituitary gland development cell differentiation neuron differentiation response to lipid somatic stem cell population maintenance eosinophil fate commitment inner ear morphogenesis positive regulation of mast cell degranulation positive regulation of blood vessel endothelial cell migration cell fate commitment fat cell differentiation negative regulation of fat cell differentiation negative regulation of myeloid cell differentiation positive regulation of erythrocyte differentiation negative regulation of macrophage differentiation positive regulation of megakaryocyte differentiation positive regulation of neuron differentiation negative regulation of Notch signaling pathway positive regulation of angiogenesis positive regulation of transcription from RNA polymerase II promoter cell maturation animal organ development neuron fate commitment homeostasis of number of cells within a tissue positive regulation of phagocytosis regulation of developmental process positive regulation of phagocytosis, engulfment definitive hemopoiesis semicircular canal development vascular wound healing positive regulation of cell migration involved in sprouting angiogenesis cochlea development GABAergic neuron differentiation positive regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis negative regulation of neural precursor cell proliferation negative regulation of endothelial cell apoptotic process regulation of forebrain neuron differentiation anatomical structure morphogenesis positive regulation of transcription, DNA-templated system development cell morphogenesis in utero embryonic development cell activation type IV hypersensitivity regulation of cytokine production positive regulation of cytokine production kidney development mesonephros development lens development in camera-type eye immune system development pro-T cell differentiation aortic valve morphogenesis cardiac right ventricle morphogenesis ventricular septum development chromatin remodeling inflammatory response humoral immune response signal transduction nervous system development axon guidance negative regulation of cell proliferation male gonad development response to xenobiotic stimulus response to virus post-embryonic development positive regulation of signal transduction response to gamma radiation gene expression positive regulation of endothelial cell migration negative regulation of epithelial to mesenchymal transition regulation of neuron projection development T cell differentiation erythrocyte differentiation macrophage differentiation regulation of epithelial cell differentiation TOR signaling negative regulation of interferon-gamma production negative regulation of interleukin-2 production positive regulation of interleukin-13 production positive regulation of interleukin-4 production positive regulation of interleukin-5 production T cell differentiation in thymus negative regulation of mammary gland epithelial cell proliferation embryonic hemopoiesis cellular response to interferon-alpha ureter maturation parathyroid hormone secretion norepinephrine biosynthetic process regulation of CD4-positive, alpha-beta T cell differentiation protein kinase B signaling regulation of neuron apoptotic process ear development response to estrogen thymic T cell selection T-helper 2 cell differentiation response to ethanol positive regulation of T cell differentiation positive regulation of cell differentiation negative regulation of cell cycle negative regulation of transcription, DNA-templated sympathetic nervous system development thymus development embryonic organ development developmental growth anatomical structure formation involved in morphogenesis homeostasis of number of cells negative regulation of inflammatory response T cell receptor signaling pathway cartilage development regulation of multicellular organismal process positive regulation of protein kinase B signaling parathyroid gland development pharyngeal system development uterus development canonical Wnt signaling pathway mesenchymal to epithelial transition mast cell differentiation ureteric bud formation cellular response to cytokine stimulus cellular response to interleukin-4 cellular response to tumor necrosis factor otic vesicle development cellular response to BMP stimulus positive regulation of ureteric bud formation nephric duct morphogenesis nephric duct formation regulation of nephron tubule epithelial cell differentiation ureter morphogenesis lymphocyte migration regulation of establishment of cell polarity negative regulation of cell motility positive regulation of T-helper 2 cell cytokine production positive regulation of thyroid hormone generation positive regulation of transcription regulatory region DNA binding regulation of cellular response to X-ray defense response
          It has to be done as per old AB suggested Products section.

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