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Invitrogen
PHD2 Recombinant Rabbit Monoclonal Antibody (0K3D3)
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FIGURE: 1 / 1
PHD2 Antibody (MA5-55367) in WB
Western blot analysis of lysates from wild type (WT) and PHD2/EGLN1 knockout (KO) 293T(KO) cells, using PHD2 Recombinant Monoclonal Antibody (Product # MA5-55367) at 1:20,000 dilution followed by a HRP-conjugated Goat anti-Rabbit IgG (H+L) secondary antibody at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 8s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-55367
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
0K3D3
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PHD2/EGLN1.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.2 mg/mL
Amount
120 µg
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
RRID
Product Specific Information
Immunogen Sequence: MANDSGGPGG PSPSERDRQY CELCGKMENL LRCSRCRSSF YCCKEHQRQD WKKHKLVCQG SEGALGHGVG PHQHSGPAPP AAVPPPRAGA REPRKAAARR
Target Information
SYMPK is a nuclear protein that functions in the regulation of polyadenylation and promotes gene expression. The protein forms a high-molecular weight complex with components of the polyadenylation machinery. It is thought to serve as a scaffold for recruiting regulatory factors to the polyadenylation complex. It also participates in 3'-end maturation of histone mRNAs, which do not undergo polyadenylation. The protein also localizes to the cytoplasmic plaques of tight junctions in some cell types.This gene encodes a nuclear protein that functions in the regulation of polyadenylation and promotes gene expression. The protein forms a high-molecular weight complex with components of the polyadenylation machinery. It is thought to serve as a scaffold for recruiting regulatory factors to the polyadenylation complex. It also participates in 3'-end maturation of histone mRNAs, which do not undergo polyadenylation. The protein also localizes to the cytoplasmic plaques of tight junctions in some cell types.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C1ORF12 antibody; Egl nine homolog 1; EGLN 1 EGLN1; HIF PH2; HIF-P4H 2; HIF-PH2; Hypoxia-inducible factor prolyl hydroxylase 2; P4H2; PHD2; Prolyl Hy; Prolyl hydroxylase domain-containing protein 2; SM-20
Gene Aliases: C1orf12; EGLN1; PNAS-118; PNAS-137
Entrez Gene ID: (Human) 54583
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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