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Invitrogen
alpha-1a Adrenergic Receptor Recombinant Rabbit Monoclonal Antibody (4G10W8)
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FIGURE: 1 / 1
alpha-1a Adrenergic Receptor Antibody (MA5-55379) in WB
Western Blot analysis of various lysates using alpha-1a Adrenergic Receptor Recombinant Monoclonal Antibody (Product # MA5-55379) at 1:1,000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-55379
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
4G10W8
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 150-250 of human ADRA1A.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
RRID
Product Specific Information
Immunogen Sequence: VWALSLVISI GPLFGWRQPA PEDETICQIN EEPGYVLFSA LGSFYLPLAI ILVMYCRVYV VAKRESRGLK SGLKTDKSDS EQVTLRIHRK NAPAGGSGMA S
Target Information
Adrenergic receptors (ARs) are members of the 7-transmembrane domain G-protein-coupled receptor superfamily that bind the endogenous catecholamines epinephrine and norepinephrine. Pharmacological, structural, and molecular cloning data indicate significant heterogeneity within this receptor family. Nine receptor subtypes have been identified thus far including three alpha-1 AR subtypes (1A/D, 1B, and 1C), three alpha-2 ARs (2A, 2B, and 2C), and three beta AR subtypes (1, 2, and 3). ARs participate in either the onset or maintenance of several disease states including hypertension, cardiac dysfunction (congestive heart failure, ischemia, arrhythmias), diabetes, glaucoma, depression, and impotence. A1AR subtypes are found in numerous tissues and have been shown to be involved in the regulation of blood pressure due to changes in vascular tone and cardiac output. Effects on uterine contraction, hepatic glucose metabolism, heat shock protein 70 (HSP70), proto-oncogene expression, and mitogenesis have been linked to A1AR activation. Norepinephrine can increase nitric oxide synthase (NOS) levels in the hypothalamus by activating alpha-1 AR which may indirectly suppress the release of luteinizing hormone (LH), the androgens, estrogen, and progesterone.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ADA1; Alpha-1A adrenergic receptor; Alpha-1A adrenoceptor; Alpha-1A adrenoreceptor; Alpha-1C adrenergic receptor; Alpha-adrenergic receptor 1c; Alpha1C-AR
Gene Aliases: ADRA1A; ADRA1C
UniProt ID: (Mouse) P97718
Entrez Gene ID: (Mouse) 11549
negative regulation of heart rate involved in baroreceptor response to increased systemic arterial blood pressure
norepinephrine-epinephrine vasoconstriction involved in regulation of systemic arterial blood pressure
positive regulation of heart rate by epinephrine-norepinephrine
positive regulation of the force of heart contraction by epinephrine-norepinephrine
positive regulation of systemic arterial blood pressure
regulation of muscle contraction
signal transduction
G-protein coupled receptor signaling pathway
phospholipase C-activating G-protein coupled receptor signaling pathway
activation of phospholipase C activity
positive regulation of cytosolic calcium ion concentration
cell-cell signaling
adult heart development
aging
response to hormone
positive regulation of heart rate
cell growth
regulation of vasoconstriction
positive regulation of synaptic transmission, GABAergic
negative regulation of Rho protein signal transduction
organ growth
response to drug
positive regulation of MAPK cascade
positive regulation of action potential
positive regulation of vasoconstriction
positive regulation of smooth muscle contraction
regulation of cardiac muscle contraction
micturition
calcium ion transport into cytosol
positive regulation of cardiac muscle contraction
positive regulation of ERK1 and ERK2 cascade
adrenergic receptor signaling pathway
adenylate cyclase-activating adrenergic receptor signaling pathway
positive regulation of protein kinase C signaling
pilomotor reflex
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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