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Invitrogen
EBP50 Recombinant Rabbit Monoclonal Antibody (24GB1270)
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FIGURE: 1 / 3
EBP50 Antibody (MA5-56946) in WB
Western blot analysis of EBP50. Total cell lysates (30 μg) from C2C12, H9c2, HAP-1, HeLa, HepG2, and HT-1080 cell lines were loaded and separated by SDS-PAGE. The blot was incubated with EBP50 Recombinant Monoclonal Antibody (Product # MA5-56945) at a 1:5,000 dilution and HRP-conjugated goat anti-rabbit secondary antibody at a 1:20,000 dilution respectively.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-56946
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293F
Class
Recombinant Monoclonal
Type
Antibody
Clone
24GB1270
Immunogen
A synthesized peptide derived from human EBP50 (aa 51-120)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Dry ice (international)
RRID
Target Information
NHERF1 (Na+/H+ exchanger regulatory factor 1), also known as EBP50 (ezrin, radixin, moesin-binding phosphoprotein 50) is an adaptor protein, which associates with beta-catenin and is required for its localization at the cell-cell junctions, interacts with various G protein-coupled receptors and regulates their traffic, as well as sodium-hydrogen exchange and sodium-dependent phosphate transport. NHERF1/EBP50 inhibits cell motility and is required to suppress anchorage-independent growth. It contains C-terminal ERM (ezrin, radixin, moesin)-binding region and two N-terminal PDZ (postsynaptic-density-95/disc-large/ZO1 homology) domains and is able to form head-to-tail intramolecular conformation to regulate its interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EBP50; ERM-binding phosphoprotein; Ezrin-radixin-moesin-binding phosphoprotein 50; Na(+)/H(+) exchange regulatory cofactor NHE-RF1; NHERF-1; Regulatory cofactor of Na(+)/H(+) exchanger; So; Sodium-hydrogen exchanger regulatory factor 1; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 1; solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1; Solute carrier family 9 isoform A3 regulatory factor 1
Gene Aliases: NHERF; NHERF1; SLC9A3R1
UniProt ID: (Rat) Q9JJ19
Entrez Gene ID: (Rat) 59114
receptor binding
protein binding
beta-catenin binding
chloride channel regulator activity
phosphatase binding
protein domain specific binding
PDZ domain binding
beta-2 adrenergic receptor binding
protein complex binding
protein complex scaffold
protein self-association
dopamine receptor binding
growth factor receptor binding
renal sodium ion transport
adenylate cyclase-activating dopamine receptor signaling pathway
sensory perception of sound
negative regulation of cell proliferation
negative regulation of platelet-derived growth factor receptor signaling pathway
negative regulation of sodium ion transport
negative regulation of phosphatidylinositol 3-kinase signaling
Wnt signaling pathway
cAMP-mediated signaling
microvillus assembly
actin cytoskeleton organization
negative regulation of cell migration
cellular phosphate ion homeostasis
negative regulation of sodium:proton antiporter activity
bile acid secretion
cellular protein localization
glutathione transport
regulation of excretion
regulation of protein kinase activity
negative regulation of protein kinase B signaling
auditory receptor cell stereocilium organization
phospholipase C-activating dopamine receptor signaling pathway
renal absorption
negative regulation of ERK1 and ERK2 cascade
renal phosphate ion absorption
negative regulation of cell motility
positive regulation of intrinsic apoptotic signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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