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Invitrogen
Eotaxin Recombinant Rabbit Monoclonal Antibody (0Z5K4)
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FIGURE: 1 / 1
Eotaxin Antibody (MA5-57969) in WB
Western blot analysis of Eotaxin in Recombinant Mouse CCL11 / Eotaxin Protein lysates (1, 5, and 10 ng/lane). Samples were incubated overnight at 4°C with Eotaxin Recombinant Monoclonal Antibody (Product # MA5-57970) using a dilution of 1:1, 000. Followed by a HRP-conjugated Goat anti-Rabbit IgG (H+L) Secondary Antibody at 1:10,000 dilution. Blocking buffer: 3% nonfat dry milk in TBST; Exposure: 20 seconds.
Product Details
MA5-57969
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
0Z5K4
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 26-97 of mouse CCL11/Eotaxin (NP_035460.1).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
3.04 mg/mL
Amount
60.8 µg
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Dry ice (international)
RRID
Product Specific Information
Immunogen sequence: GSIPTSCCFI MTSKKIPNTL LKSYKRITNN RCTLKAIVFK TRLGKEICAD PKKKWVQDAT KHLDQKLQTP KP
Target Information
This gene is one of several Cys-Cys (CC) cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for eosinophils, but not mononuclear cells or neutrophils. This eosinophil specific chemokine assumed to be involved in eosinophilic inflammatory diseases such as atopic dermatitis, allergic rhinitis, asthma and parasitic infections.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-C motif chemokine 11; Eosinophil Chemotactic Factor; Eosinophil chemotactic protein; Eotaxin; Eotaxin 1; Eotaxin1; MGC22554; small chemokine (C-C motif) ligand 11; small inducible cytokine A11; Small-inducible cytokine A11
Gene Aliases: Ccl11; eotaxin; Scya11
UniProt ID: (Mouse) P48298
Entrez Gene ID: (Mouse) 20292
positive regulation of endothelial cell proliferation
mast cell chemotaxis
chemotaxis
inflammatory response
immune response
cytoskeleton organization
actin filament organization
regulation of cell shape
positive regulation of cell migration
positive regulation of actin filament polymerization
positive regulation of GTPase activity
positive regulation of angiogenesis
eosinophil chemotaxis
branching involved in mammary gland duct morphogenesis
mammary duct terminal end bud growth
ERK1 and ERK2 cascade
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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