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Invitrogen
ASAH1 Recombinant Rabbit Monoclonal Antibody (PSH07-71)
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图: 1 / 7
ASAH1 Antibody (MA562087) in ICC/IF
Immunofluorescence analysis of ASAH1 was performed using 70% confluent log phase SK-MEL-5 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with ASAH1 Recombinant Rabbit Monoclonal Antibody (PSH07-71) (Product # MA562087) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2,000 dilution), f... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
MA562087
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
PSH07-71
抗原
Recombinant protein within human ASAH1 aa 1-395.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
Protein A
保存液
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Positive Control: SK-MEL-28 cell lysate, MDA-MB-231 cell lysate, SH-SY5Y cell lysate, human brain tissue, human kidney tissue.
Tissue Specificity: Tissue enhanced (heart).
Subcellular Location: Lysosome, Secreted; Nucleus, Cytoplasm.
Sequence Similarities: 79%Mouse/82%Rat.
Predicted band size: 45 kDa.
靶标信息
ASAH1 encodes a member of the acid ceramidase family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. The processing of this preproprotein generates alpha and beta subunits that heterodimerize to form the mature lysosomal enzyme, which catalyzes the degradation of ceramide into sphingosine and free fatty acid. This enzyme is overexpressed in multiple human cancers and may play a role in cancer progression. Mutations in this gene have been linked to the lysosomal storage disorder, Farber lipogranulomatosis, and a neuromuscular disorder, spinal muscular atrophy with progressive myoclonic epilepsy. The related pathways include Innate Immune System and Sphingolipid metabolism. Diseases associated with ASAH1 include Farber Lipogranulomatosis and Spinal Muscular Atrophy With Progressive Myoclonic Epilepsy.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AC; Acid ceramidase; Acylsphingosine deacylase; Glycosylceramide deacylase; N-acylethanolamine hydrolase ASAH1; N-acylsphingosine amidohydrolase; PHP32; Putative 32 kDa heart protein
基因别名: ASAH; ASAH1; HSD-33; HSD33
Entrez Gene ID: (Human) 427
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