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          • Primary Antibodies ›
          • LYN/LCK/HCK/BLK Antibodies

          Invitrogen

          Phospho-LYN/LCK/HCK/BLK (Tyr397, Tyr394, Tyr411, Tyr389) Recombinant Rabbit Monoclonal Antibody (PSH09-43)

          高级验证

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          Cite Phospho-LYN/LCK/HCK/BLK (Tyr397, Tyr394, Tyr411, Tyr389) Recombinant Rabbit Monoclonal Antibody (PSH09-43)

          Additional Information:

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          • 抗体检测数据 (5)
          • 高级验证 (3)
          Phospho-LYN/LCK/HCK/BLK (Tyr397, Tyr394, Tyr411, Tyr389) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Phospho-LYN/LCK/HCK/BLK (Tyr397, Tyr394, Tyr411, Tyr389) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          图: 1 / 8

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          Phospho-LYN/LCK/HCK/BLK (Tyr397, Tyr394, Tyr411, Tyr389) Antibody (MA562902) in ICC/IF

          Immunofluorescence analysis of Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) using Ramos cells serum starved for 16 hours then treated with 12μg/mL human IgM (diluted in serum free medium) for 2 minutes. The cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative... View More {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
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          Phospho-LYN/LCK/HCK/BLK (Tyr397, Tyr394, Tyr411, Tyr389) Antibody in Immunocytochemistry (ICC/IF)
          Phospho-LYN/LCK/HCK/BLK (Tyr397, Tyr394, Tyr411, Tyr389) Antibody in Western Blot (WB)
          Phospho-LYN/LCK/HCK/BLK (Tyr397, Tyr394, Tyr411, Tyr389) Antibody in Western Blot (WB)
          Phospho-LYN/LCK/HCK/BLK (Tyr397, Tyr394, Tyr411, Tyr389) Antibody in Western Blot (WB)
          Phospho-LYN/LCK/HCK/BLK (Tyr397, Tyr394, Tyr411, Tyr389) Antibody in Western Blot (WB)
          Phospho-LYN/LCK/HCK/BLK (Tyr397, Tyr394, Tyr411, Tyr389) Antibody
          Phospho-LYN/LCK/HCK/BLK (Tyr397, Tyr394, Tyr411, Tyr389) Antibody
          Phospho-LYN/LCK/HCK/BLK (Tyr397, Tyr394, Tyr411, Tyr389) Antibody

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          Phospho-LYN/LCK/HCK/BLK (Tyr397, Tyr394, Tyr411, Tyr389) Recombinant Rabbit Monoclonal Antibody (PSH09-43)

          产品信息

          MA562902

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          1:2,000
          -

          免疫细胞化学 (ICC/IF)

          1:100
          -
          产品规格

          种属反应

          Human, Mouse, Rat

          宿主/亚型

          Rabbit / IgG

          Expression System

          HEK293 cells

          分类

          Recombinant Monoclonal

          类型

          Antibody

          克隆号

          PSH09-43

          抗原

          Synthetic phospho-peptide corresponding to residues surrounding Tyr394 of human LCK.
          3D表位/免疫原

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Liquid

          浓度

          1 mg/mL

          规格

          100 µg

          纯化类型

          Protein A

          保存液

          PBS, pH 7.4, with 0.1% BSA, 40% glycerol

          内含物

          0.05% sodium azide

          保存条件

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_3719757

          产品详细信息

          Positive Control: COLO205 cell lysate, TT cell lysate, Ramos serum starved for 16 hours cell lysate, Ramos serum starved for 16 hours add 12 µg/mL human IgM (diluted in serum free medium) for 2 minutes cell lysate, NIH/3T3 cell lysate, NIH/3T3 treated with 50mM sodium orthovanadate for 5 minutes cell lysate, PC-12 cell lysate, PC-12 treated with 1mM sodium orthovanadate for 30 minutes cell lysate, Ramos cells serum starved for 16 hours add 12 µg/mL human IgM (diluted in serum free medium) for 2 minutes.

          Tissue Specificity: Tissue enhanced (bone marrow, lymphoid tissue).

          Subcellular Location: Cell membrane, Cytoplasm, cytosol.

          Sequence Similarities: 100% Mouse/Rat.

          Predicted band size: 58 kDa

          靶标信息

          The LYN, LCK, HCK, and BLK genes encode various members of the Src family of non-receptor tyrosine kinases, each playing crucial roles in cellular signaling pathways that regulate immune function, cell proliferation, and differentiation. LYN is involved in B-cell receptor signaling, influencing cell proliferation, differentiation, apoptosis, migration, metabolism, and roles in inflammation and cancer, particularly within the immune system and oncogenic pathways. LCK is primarily expressed in T-cells and is crucial for T-cell development, function, and signaling, impacting cytokine production and immune responses. HCK, primarily found in hematopoietic cells, influences myeloid cell function and is implicated in oncogenic signaling pathways, potentially contributing to cancer progression via altered immune responses. BLK participates mainly in B-cell receptor signaling, impacting B-cell development and activation, with implications for immune cell signaling and potential roles in autoimmune diseases. Each of these kinases is a potential therapeutic target, given their roles in various pathophysiological conditions and their impact on immune cell signaling and cancer.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          篇参考文献 (0)

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          Cite this product

          生物信息学

          蛋白别名: B cell/myeloid kinase; B lymphocyte kinase; B lymphoid kinase; B lymphoid tyrosine kinase; Blk; BLK nonreceptor tyrosine kinase; hematopoietic cell kinase; hemopoietic cell kinase; hemopoietic cell tyrosine kinase; lck/Yes-related novel protein tyrosine kinase; leukocyte C-terminal Src kinase; lymphocyte cell-specific protein-tyrosine kinase; lymphocyte protein tyrosine kinase; lymphocyte-specific protein tyrosine kinase; lyn; lyn protein non-receptor kinase; lyn tyrosine kinase; p55-Blk; p56(LSTRA) protein-tyrosine kinase; p56-HCK; p56-HCK/p59-HCK; p59-HCK/p60-HCK; protein tyrosine kinase; protein-tyrosine kinase; protein-tyrosine kinase (EC 2.7.1.112); proto-oncogene tyrosine-protein kinase LCK; Src-family protein tyrosine kinase; t cell-specific protein-tyrosine kinase; T-lymphocyte specific protein tyrosine kinase p56lck; unnamed protein product; V-yes-1 Yamaguchi sarcoma viral related oncogene homolog; Yamaguchi sarcoma viral (v-yes-1) oncogene homolog

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          基因别名: AIPCV; Bmk; Hck-2; Hck-3; Hctk; IMD22; JTK8; JTK9; Lck1; Lcktkr; LSK; Lskt; MODY11; p53Lyn; p56-LCK; p56; p56Hck; p56Lck; p56Lyn; p59Hck; p61Hck; pp58lck; SAIDV; YT16

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          UniProt ID: (Human) P51451, (Mouse) P16277, (Human) P08631, (Rat) P50545, (Mouse) P08103, (Human) P06239, (Human) P07948, (Rat) Q01621, (Mouse) P06240, (Mouse) P25911, (Rat) Q07014

          查看更多 收起

          Entrez Gene ID: (Human) 640, (Mouse) 12143, (Rat) 364403, (Human) 3055, (Rat) 25734, (Mouse) 15162, (Human) 3932, (Human) 4067, (Rat) 313050, (Mouse) 16818, (Mouse) 17096, (Rat) 81515

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          功能
          protein kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity receptor binding protein binding ATP binding nucleotide binding phosphotyrosine binding lipid binding protein serine/threonine phosphatase activity phospholipase activator activity protein kinase binding protein phosphatase binding SH2 domain binding T cell receptor binding CD4 receptor binding CD8 receptor binding identical protein binding phospholipase binding phosphatidylinositol 3-kinase binding ATPase binding protein antigen binding platelet-derived growth factor receptor binding integrin binding kinase activity enzyme binding receptor activator activity ubiquitin protein ligase binding immunoglobulin receptor binding gamma-tubulin binding glycosphingolipid binding ion channel binding macromolecular complex binding ephrin receptor binding phosphoprotein binding scaffold protein binding phosphorylation-dependent protein binding antigen binding erythropoietin receptor binding SH3 domain binding phosphatidylinositol 3-kinase activator activity
          参与通路
          leukocyte migration involved in immune response innate immune response-activating signal transduction negative regulation of inflammatory response to antigenic stimulus protein phosphorylation defense response cell adhesion transmembrane receptor protein tyrosine kinase signaling pathway integrin-mediated signaling pathway mesoderm development positive regulation of cell proliferation regulation of cell shape peptidyl-tyrosine phosphorylation cytokine-mediated signaling pathway cell differentiation positive regulation of actin filament polymerization lipopolysaccharide-mediated signaling pathway regulation of actin cytoskeleton organization intracellular signal transduction Fc-gamma receptor signaling pathway involved in phagocytosis negative regulation of apoptotic process leukocyte degranulation respiratory burst after phagocytosis protein autophosphorylation regulation of inflammatory response regulation of phagocytosis leukocyte migration interferon-gamma-mediated signaling pathway cellular response to lipid regulation of podosome assembly positive regulation of insulin secretion B cell receptor signaling pathway DNA damage checkpoint cell morphogenesis B cell homeostasis regulation of cytokine production negative regulation of protein phosphorylation hematopoietic progenitor cell differentiation Fc receptor mediated stimulatory signaling pathway tolerance induction to self antigen histamine secretion by mast cell platelet degranulation negative regulation of myeloid leukocyte differentiation immune response-regulating cell surface receptor signaling pathway Fc receptor mediated inhibitory signaling pathway regulation of B cell apoptotic process autophagy inflammatory response cellular response to DNA damage stimulus negative regulation of cell proliferation response to hormone positive regulation of neuron projection development fatty acid transport vesicle-mediated transport hemopoiesis erythrocyte differentiation eosinophil differentiation positive regulation of cell migration negative regulation of B cell proliferation neuron projection development regulation of mast cell activation regulation of cell adhesion mediated by integrin negative regulation of toll-like receptor 2 signaling pathway toll-like receptor 4 signaling pathway negative regulation of toll-like receptor 4 signaling pathway positive regulation of toll-like receptor 4 signaling pathway positive regulation of toll-like receptor 9 signaling pathway interleukin-5-mediated signaling pathway C-X-C chemokine receptor CXCR4 signaling pathway B cell proliferation positive regulation of phosphorylation hemoglobin biosynthetic process regulation of mast cell degranulation negative regulation of MAP kinase activity positive regulation of MAPK cascade regulation of erythrocyte differentiation ephrin receptor signaling pathway regulation of B cell receptor signaling pathway negative regulation of B cell receptor signaling pathway regulation of release of sequestered calcium ion into cytosol positive regulation of glial cell proliferation positive regulation of Fc receptor mediated stimulatory signaling pathway regulation of ERK1 and ERK2 cascade negative regulation of ERK1 and ERK2 cascade positive regulation of oligodendrocyte progenitor proliferation negative regulation of mast cell proliferation positive regulation of mast cell proliferation cellular response to retinoic acid regulation of monocyte chemotaxis regulation of platelet aggregation dendritic cell differentiation neuroinflammatory response negative regulation of intracellular signal transduction positive regulation of amyloid precursor protein catabolic process positive regulation of protein localization to plasma membrane positive regulation of dendritic cell apoptotic process response to sterol depletion response to xenobiotic stimulus response to toxic substance response to carbohydrate oligodendrocyte development response to insulin cellular response to heat response to amino acid response to peptide hormone response to axon injury cell surface receptor signaling pathway response to mechanical stimulus response to metal ion response to zinc ion positive regulation of gene expression T cell differentiation positive regulation of heterotypic cell-cell adhesion Fc-gamma receptor signaling pathway T cell activation positive regulation of tyrosine phosphorylation of STAT protein response to hydrogen peroxide positive regulation of gamma-delta T cell differentiation regulation of regulatory T cell differentiation T cell receptor signaling pathway regulation of T cell receptor signaling pathway positive regulation of T cell activation NK T cell activation release of sequestered calcium ion into cytosol positive regulation of multicellular organismal process positive regulation of uterine smooth muscle contraction CD27 signaling pathway positive regulation of leukocyte cell-cell adhesion response to aldosterone positive regulation of intrinsic apoptotic signaling pathway regulation of protein phosphorylation stimulatory C-type lectin receptor signaling pathway signal transduction T cell costimulation Fc-epsilon receptor signaling pathway negative regulation of immune response JAK-STAT cascade involved in growth hormone signaling pathway phagocytosis defense response to Gram-positive bacterium biological_process immune response-activating cell surface receptor signaling pathway platelet activation positive regulation of T cell receptor signaling pathway regulation of lymphocyte activation
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