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Invitrogen
COL11A1 Recombinant Rabbit Monoclonal Antibody (JE40-84)
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FIGURE: 1 / 5
COL11A1 Antibody (MA562908) in ICC/IF
Immunofluorescence analysis of COL11A1 was performed using Saos-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with COL11A1 Recombinant Rabbit Monoclonal Antibody (JE40-84) (Product # MA562908) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2,000 dilution), for 45 minutes at room t... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA562908
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JE40-84
Immunogen
Recombinant protein within Human COL11A1 aa 501-600/1,806.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 0.05% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Control: K-562 cell lysate, Jurkat cell lysate, PANC-1 cell lysate, HCT 116 cell lysate.
Tissue Specificity: Tissue enhanced (placenta, retina).
Subcellular Location: Secreted, extracellular space, extracellular matrix.
Sequence Similarities: 97% Mouse/Rat.
Predicted band size: 181 kDa
Target Information
Collagen Type XI is an 1806 amino acid protein belonging to the fibrillar collagen family. Collagen Type XI is thought to play an important role in fibrillogenesis by controlling lateral growth of collagen II fibrils. This protein forms trimers composed of three different chains: a 1(XI), a 2(XI), and a 3(XI). a 3(XI) is a post-translational modification of a 1(II). a 1(V) can also be found instead of a 3(XI). Collagen Type XI has three named isoforms (A,B,C) and additional isoforms seem to exist, stemming from alternative usage of exon IIA or exon IIB. Transcripts containing exon IIA or IIB are present in cartilage, but exon IIB is preferentially utilized in transcripts from tendon. Collagen Type XI contains a single collagen binding TSP N-terminal (TSPN) domain. Collagen Type XI is expressed in cartilage, placenta and some tumor or virally transformed cell lines. Isoform expression can be tissue specific. Defects in the COL11A gene are the cause of Stickler syndrome type 2 (STL2), or beaded vitreous type, due to the presence of irregularly thickened fiber bundles throughout vitreous cavity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: alpha-1 (type XI) collagen precursor; COBA1; Collagen alpha 1; collagen XI, alpha-1 polypeptide; collagen, type XI, alpha 1; deafness, autosomal dominant 37; STL3; unnamed protein product; XI chain precursor
Gene Aliases: CO11A1; COLL6; DFNA37; STL2
UniProt ID: (Human) P12107
Entrez Gene ID: (Human) 1301
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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