Invitrogen

p19ARF Recombinant Rabbit Monoclonal Antibody (0N7N4)

查看其他6个p19ARF抗体

问题与答案

问题与答案

问题与答案

Additional Information:

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抗体检测数据 (3)

p19ARF Antibody in Immunocytochemistry (ICC/IF)

p19ARF Antibody in Immunocytochemistry (ICC/IF)

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p19ARF Antibody (MA569313) in ICC/IF

Immunocytochemical analysis of p19ARF in A20 cells. Samples were incubated with p19ARF recombinant monoclonal antibody (Product # MA569312) using a dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) using a dilution of 1:500. DAPI for nuclear staining (Blue).

p19ARF Antibody in Immunocytochemistry (ICC/IF)

p19ARF Antibody in Immunohistochemistry (Paraffin) (IHC (P))

p19ARF Antibody in Western Blot (WB)

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p19ARF Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemical analysis of p19ARF in A20 cells. Samples were incubated with p19ARF recombinant monoclonal antibody (Product # MA569312) using a dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) using a dilution of 1:500. DAPI for nuclear staining (Blue).

p19ARF Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of p19ARF on paraffin-embedded Mouse testis using CDKN2A/p19ARF Rabbit mAb tissue. Samples were incubated with p19ARF recombinant monoclonal antibody (Product # MA569312) using a dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

p19ARF Antibody in Western Blot (WB) — Western blot analysis of p19ARF in A20 cells. Samples were probed with p19ARF recombinant monoclonal antibody (Product # MA569312) at 1:2,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

产品信息

MA569313

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:6,000

-

免疫组化（石蜡） (IHC (P))

1:200-1:800

-

免疫细胞化学 (ICC/IF)

1:200-1:800

-

酶联免疫吸附实验 (ELISA)

1 µg/mL

-

产品规格

种属反应

Mouse

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

0N7N4

抗原

Synthetic peptide.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.68 mg/mL

33.6 µg

纯化类型

Affinity chromatography

保存液

PBS, pH 7.3., with 50% glycerol

内含物

0.09% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

In humans, the multiple tumor suppressor 1 locus (MTS1) encodes two unrelated genes, p16INK4a and p19ARF. Although both act as cell proliferation inhibitors, their mechanisms of action are different. p19ARF works as part of a p53-dependent pathway to counter uncontrolled proliferation and oncogenic signals. Mice lacking the p19ARF gene rapidly develop a broad spectrum of tumors. This result indicates that p19ARF is an important tumor suppressor. In addition, p19ARF helps prevent transforming signals from various onocoproteins via the p53 regulatory loop. These studies and others display the importance of p19ARF in cell cycle control and tumor suppression.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Alternative reading frame; ARF; CDK4I; cdkn2a; Cyclin-dependent kinase 4 inhibitor A; Cyclin-dependent kinase inhibitor 2A; cyclin-dependent kinase inhibitor 2A (p16, inhibits CDK4); cyclin-dependent kinase inhibitor 2A, isoform 3; cyclin-dependent kinase inhibitor 2A, isoforms 1/2; cyclin-dependent kinase inhibitor protein; mitochondrial smARF; p16-INK4; p16-INK4a; p19ARF; Tumor suppressor ARF

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基因别名: Arf; ARF-INK4a; Cdkn2a; INK4a-ARF; Ink4a/Arf; MTS1; p16; p16(INK4a); P16ink4a; p19; p19ARF; Pctr1

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UniProt ID: (Mouse) P51480

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Entrez Gene ID: (Mouse) 12578

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p53 binding DNA binding transcription factor activity, sequence-specific DNA binding cyclin-dependent protein serine/threonine kinase inhibitor activity protein binding transcription factor binding protein kinase binding poly(A) RNA binding protein N-terminus binding NF-kappaB binding ubiquitin-protein transferase inhibitor activity MDM2/MDM4 family protein binding

regulation of cyclin-dependent protein serine/threonine kinase activity G1/S transition of mitotic cell cycle protein polyubiquitination mitophagy negative regulation of cell-matrix adhesion transcription, DNA-templated regulation of transcription, DNA-templated rRNA processing negative regulation of protein kinase activity apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process cell cycle cell cycle arrest aging cell aging negative regulation of cell proliferation epidermis development apoptotic mitochondrial changes rRNA transcription response to organonitrogen compound regulation of G2/M transition of mitotic cell cycle regulation of gene expression response to organic cyclic compound negative regulation of cell growth negative regulation of B cell proliferation regulation of protein stability protein destabilization negative regulation of NF-kappaB transcription factor activity negative regulation of protein binding negative regulation of immature T cell proliferation in thymus positive regulation of protein sumoylation negative regulation of mammary gland epithelial cell proliferation positive regulation of smooth muscle cell apoptotic process somatic stem cell population maintenance senescence-associated heterochromatin focus assembly negative regulation of phosphorylation response to drug glucose homeostasis positive regulation of apoptotic process positive regulation of DNA damage response, signal transduction by p53 class mediator negative regulation of cyclin-dependent protein serine/threonine kinase activity negative regulation of cell cycle negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter regulation of nucleocytoplasmic transport regulation of protein export from nucleus somatic stem cell division protein stabilization positive regulation of sequence-specific DNA binding transcription factor activity negative regulation of ubiquitin-protein transferase activity mitochondrial depolarization positive regulation of apoptotic process involved in mammary gland involution cellular response to hydrogen peroxide protein K63-linked ubiquitination positive regulation of cell cycle arrest cellular senescence replicative senescence positive regulation of protein localization to nucleus positive regulation of signal transduction by p53 class mediator regulation of apoptotic DNA fragmentation negative regulation of proteolysis involved in cellular protein catabolic process regulation of protein targeting to mitochondrion negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process positive regulation of macrophage apoptotic process positive regulation of cellular senescence