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Invitrogen
eIF4E Recombinant Rabbit Monoclonal Antibody (4N7U2)
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图: 1 / 8
eIF4E Antibody (MA569428) in ICC/IF
Immunocytochemical analysis of eIF4E in NIH/3T3 cells. Samples were incubated with eIF4E recombinant monoclonal antibody (Product # MA569428) using a dilution of 1:200 (40x lens). Followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) using a dilution of 1:500 (Red). The cells were counterstained with alpha-Tubulin Mouse mAb using dilution of 1:400. Followed by incubation with 488-conjugated Goat Anti-Mouse IgG (H+L) using a dilution of 1:500 (Green). DAPI was used for nuclear staining (Blue).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
MA569428
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Monkey,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
4N7U2
抗原
Synthetic peptide.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
2.09 mg/mL
规格
209 µg
纯化类型
Affinity chromatography
保存液
PBS, pH 7.3., with 50% glycerol
内含物
0.09% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
靶标信息
eIF4E is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A, that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: eIF-4E; eIF-4F 25 kDa subunit; Eukaryotic translation initiation factor 4E; MGC111573; mRNA cap-binding protein; OTTHUMP00000219703; OTTHUMP00000219704; OTTHUMP00000219705
基因别名: EG668879; eIF-4E; EIF4E; Eif4e-ps; EIF4EL1; EIF4F; If4e
UniProt ID: (Mouse) P63073, (Rat) P63074
Entrez Gene ID: (Mouse) 13684, (Rat) 117045
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