Invitrogen

RBM7 Recombinant Rabbit Monoclonal Antibody (1J10Z3)

查看其他5个RBM7抗体

问题与答案

问题与答案

问题与答案

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抗体检测数据 (9)

RBM7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RBM7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 9

RBM7 Antibody (MA569696) in IHC (P)

Immunohistochemistry analysis of RBM7 on paraffin-embedded Mouse testis tissue. Samples were incubated with RBM7 recombinant monoclonal antibody (Product # MA569696) using a dilution of 1:200 (40x lens). Followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) using a dilution of 1:500 (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0. 01M Citrate... View More

RBM7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RBM7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RBM7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RBM7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RBM7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RBM7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RBM7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RBM7 Antibody in Western Blot (WB)

RBM7 Antibody in Immunoprecipitation (IP)

Product Image Gallery

RBM7 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of RBM7 on paraffin-embedded Mouse testis tissue. Samples were incubated with RBM7 recombinant monoclonal antibody (Product # MA569696) using a dilution of 1:200 (40x lens). Followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) using a dilution of 1:500 (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0).

RBM7 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of RBM7 on paraffin-embedded Mouse colon tissue. Samples were incubated with RBM7 recombinant monoclonal antibody (Product # MA569696) using a dilution of 1:200 (40x lens). Followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) using a dilution of 1:500 (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0).

RBM7 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of RBM7 on paraffin-embedded Rat testis tissue. Samples were incubated with RBM7 recombinant monoclonal antibody (Product # MA569696) using a dilution of 1:200 (40x lens). Followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) using a dilution of 1:500 (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0).

RBM7 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of RBM7 on paraffin-embedded Rat colon tissue. Samples were incubated with RBM7 recombinant monoclonal antibody (Product # MA569696) using a dilution of 1:200 (40x lens). Followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) using a dilution of 1:500 (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0).

RBM7 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of RBM7 on paraffin-embedded Rat kidney tissue. Samples were incubated with RBM7 recombinant monoclonal antibody (Product # MA569696) using a dilution of 1:5,000 (40x lens). High pressure antigen retrieval performed with 0. 01 M Tris-EDTA Buffer (pH 9. 0).

RBM7 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of RBM7 on paraffin-embedded Mouse lung tissue. Samples were incubated with RBM7 recombinant monoclonal antibody (Product # MA569696) using a dilution of 1:5,000 (40x lens). High pressure antigen retrieval performed with 0. 01 M Tris-EDTA Buffer (pH 9. 0).

RBM7 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of RBM7 on paraffin-embedded Rat colon tissue. Samples were incubated with RBM7 recombinant monoclonal antibody (Product # MA569696) using a dilution of 1:5,000 (40x lens). High pressure antigen retrieval performed with 0. 01 M Tris-EDTA Buffer (pH 9. 0).

RBM7 Antibody in Western Blot (WB) — Western blot analysis of RBM7 in wild type (WT) and Rbm7 mouse testis. Samples were probed with RBM7 recombinant monoclonal antibody (Product # MA569696) at 1:5,000 dilution incubated at room temperature for 1. 5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

RBM7 Antibody in Immunoprecipitation (IP) — Immunoprecipitation analysis of RBM7 was performed using 0.5 µg of RBM7 recombinant monoclonal antibody (Product # MA569696). Rabbit IgG isotype control was used to precipitate the Control IgG sample. Western blot analysis of immunoprecipitates was conducted using RBM7 at a 1:4,000 dilution.

产品信息

MA569696

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,500-1:10,000

-

免疫组化（石蜡） (IHC (P))

1:2,000-1:8,000

-

免疫细胞化学 (ICC/IF)

1:200-1:1,000

-

酶联免疫吸附实验 (ELISA)

1 µg/mL

-

免疫沉淀 (IP)

0.5-4 µg

-

产品规格

种属反应

Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

1J10Z3

抗原

Recombinant protein (or fragment).

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.15 mg/mL

115 µg

纯化类型

Affinity chromatography

保存液

PBS, pH 7.3., with 50% glycerol

内含物

0.09% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

RBM7 contains 1 RRM (RNA recognition motif) domain. It is possible involved in germ cell RNA processing and meiosis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: rna-binding; RNA-binding motif protein 7; RNA-binding protein 7

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基因别名: 1200007M24Rik; 1500011D06Rik; AU041934; AW554393; Rbm7

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UniProt ID: (Mouse) Q9CQT2

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Entrez Gene ID: (Mouse) 67010, (Rat) 315634

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nucleotide binding nucleic acid binding RNA binding poly(A) RNA binding nucleic acid metabolism protein

biological_process meiotic cell cycle