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Caspase 8 (Cleaved Asp384) Recombinant Rabbit Monoclonal Antibody (5T10V2)
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Caspase 8 (Cleaved Asp384) Antibody (MA569795) in WB
Western blot analysis of Caspase 8 (Cleaved Asp384) in Jurkat cells. Samples were probed with Caspase 8 (Cleaved Asp384) recombinant monoclonal antibody (Product # MA569794) at 1:5,000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 30 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
MA569795
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
5T10V2
抗原
Synthetic peptide.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
4 mg/mL
规格
80 µg
纯化类型
Affinity chromatography
保存液
PBS, pH 7.3., with 50% glycerol
内含物
0.09% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
靶标信息
Caspase-8 (cleaved Asp384) refers to the activated form of caspase-8 generated following proteolytic cleavage at aspartic acid residue 384, a key step in the activation of this initiator caspase in the extrinsic apoptosis pathway. Caspase-8 is encoded by the CASP8 gene and is synthesized as an inactive zymogen (pro-caspase-8) containing tandem death effector domains (DEDs) that enable recruitment to death-inducing signaling complexes (DISCs) formed downstream of death receptors such as Fas (CD95), TRAIL receptors, and TNF receptor 1. Upon DISC assembly, pro-caspase-8 undergoes dimerization and autocatalytic cleavage at specific aspartic acid sites, including Asp384, producing active subunits that assemble into the functional enzyme. Activated caspase-8 initiates the apoptotic cascade by cleaving and activating downstream effector caspases such as caspase-3 and caspase-7, and can also process BID to link extrinsic and intrinsic apoptosis pathways. Detection of cleaved caspase-8 (Asp384) is widely used as a marker of death receptor-mediated apoptotic signaling in cell death and cancer research.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Apoptotic cysteine protease; Apoptotic protease Mch-5; CAP4; CASP-8; Caspase-8; Caspase-8 (Asp384 cleaved); Cleaved Caspase-8; FADD-homologous ICE/ced-3-like protease; FADD-like ICE; FLICE; ICE-like apoptotic protease 5; MACH; MORT1-associated ced-3 homolog
基因别名: CASP8; MCH5
Entrez Gene ID: (Human) 841
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