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Invitrogen
Phospho-ASK1 (Thr845) Recombinant Rabbit Monoclonal Antibody (10C10C9)
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FIGURE: 1 / 2
Phospho-ASK1 (Thr845) Antibody (MA569930) in WB
Western blot analysis of Phospho-ASK1 (Thr845) in wild type (WT) and 293T cells. Samples were probed with Phospho-ASK1 (Thr845) recombinant monoclonal antibody (Product # MA569930) at 1:5,000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 30 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Product Details
MA569930
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
10C10C9
Immunogen
Synthetic peptide.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.13 mg/mL
Amount
113 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3., with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
MAP3K5 (ASK1) is a serine/threonine kinase that is abundantly expressed in human heart and pancreas and are activated in response to various extracellular stimuli, including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was identified and designated ASK1 (for apoptosis signal-regulating kinase 1) and MAPKKK5. ASK1 activates two different subgroups of MAPKK, MKK4 and MKK6, which in turn activates c-Jun N-terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1/MAPKKK5 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas-associated protein Daxx. Overexpression of ASK1 induced apoptotic cell death and a catalytically inactive form of ASK1 inhibited TNF-a-induced apoptosis. ASK1 is expressed in variety of human and mouse tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Apoptosis signal-regulating kinase 1; ASK 1; ASK-1; EC 2.7.11.25; kinase ASK1; M3K5; MAPK/ERK kinase kinase 5; MEK kinase 5; MEKK 5; mitogen activated protein kinase kinase kinase 5; Mitogen-activated protein kinase kinase kinase 5; RP3-325F22.4
Gene Aliases: 7420452D20Rik; ASK; ASK1; Map3k5; MAPKKK5; Mekk5
UniProt ID: (Mouse) O35099
Entrez Gene ID: (Mouse) 26408
nucleotide binding
magnesium ion binding
protein kinase activity
protein serine/threonine kinase activity
MAP kinase kinase kinase activity
protein binding
ATP binding
cysteine-type endopeptidase activator activity involved in apoptotic process
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
protein kinase binding
protein phosphatase binding
protein homodimerization activity
metal ion binding
non-receptor serine/threonine protein kinase
MAPK cascade
activation of MAPKK activity
activation of MAPK activity
positive regulation of protein phosphorylation
immune system process
protein phosphorylation
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
JNK cascade
intrinsic apoptotic signaling pathway in response to oxidative stress
regulation of cell death
phosphorylation
response to endoplasmic reticulum stress
positive regulation of apoptotic process
positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
positive regulation of JUN kinase activity
innate immune response
positive regulation of JNK cascade
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
cellular response to hydrogen peroxide
programmed necrotic cell death
positive regulation of neuron death
cellular response to reactive nitrogen species
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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