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Invitrogen
TDP-43 Recombinant Rabbit Monoclonal Antibody (PSH09-14)
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FIGURE: 1 / 10
TDP-43 Antibody (MA570074) in ICC/IF
Immunocytochemistry analysis of TDP-43 in NIH/3T3 cells. Samples were incubated with TDP-43 monoclonal antibody (Product # MA570074) using a dilution of 1:500 in 1% BSA in PBST overnight at 4°C followed by Goat Anti-Rabbit IgG H&L (iFluor™ 488) at a dilution of 1:1,000. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. PBS instead of the primary antibod... View More
Product Details
MA570074
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Cynomolgus monkey,
Human,
Mouse,
Pig,
Rat,
Zebrafish
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
PSH09-14
Immunogen
Synthetic peptide within human TDP43 aa 365-414.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Positive control: HeLa cell lysate, K-562 cell lysate, Neuro-2a cell lysate, Mouse spleen tissue lysate, Mouse brain tissue lysate, Rat spleen tissue lysate, Rat brain tissue lysate, NIH/3T3, human brain tissue, mouse brain tissue, rat brain tissue.
Subcellular location: Cytoplasm, Mitochondrion, Nucleus.
Recommended dilution for IF-tissue samples: 1:500
Target Information
TDP43 was first identified as a novel cellular protein that binds to HIV-1 virus TAR DNA sequence motifs and acts a transcriptional repressor to the HIV-1 LTR. Later experiments revealed that TDP43 also regulates the splicing of exon 9 of the cystic fibrosis transmembrane conductance regular (CFTR), most likely through the association with the UG repeats at the 3"e; splice site. A hyperphosphorylated, ubiquitinated, and cleaved form of TDP43 known as pathologic TDP43 is the major disease protein in ubiquitin-positive, tau-, and alpha-synuclein-negative frontotemporal dementia (FLTD-U). TDP43 is not related to TRBP1, and RNA binding protein that binds HIV-1 TAR RNA sequences.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: OTTHUMP00000002171; OTTHUMP00000002173; RP4-635E18.2; TAR DNA-binding protein 43; TAR DNA-binding protein-43; TARDBP; TDP-43; unnamed protein product
Gene Aliases: 1190002A23Rik; ALS10; C85084; TARDBP; TDP-43; TDP43; wu:fb77f02; wu:fc52g10
UniProt ID: (Human) Q13148, (Mouse) Q921F2
Entrez Gene ID: (Pig) 100739753, (Cynomolgus monkey) 101926677, (Human) 23435, (Mouse) 230908, (Rat) 298648, (Zebrafish) 325052
RNA polymerase II core promoter proximal region sequence-specific DNA binding
nucleic acid binding
DNA binding
double-stranded DNA binding
RNA binding
mRNA 3'-UTR binding
protein binding
lipid binding
identical protein binding
pre-mRNA intronic binding
molecular condensate scaffold activity
nucleotide binding
transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding
poly(A) RNA binding
negative regulation of protein phosphorylation
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
mRNA processing
RNA splicing
negative regulation of gene expression
nuclear fragmentation involved in apoptotic nuclear change
negative regulation by host of viral transcription
positive regulation of transcription from RNA polymerase II promoter
regulation of cell cycle
3'-UTR-mediated mRNA stabilization
nuclear inner membrane organization
positive regulation of insulin secretion
response to endoplasmic reticulum stress
gene expression
regulation of gene expression
regulation of protein stability
positive regulation of protein import into nucleus
regulation of circadian rhythm
regulation of apoptotic process
rhythmic process
3'-UTR-mediated mRNA destabilization
amyloid fibril formation
axonogenesis
skeletal muscle tissue development
neuromuscular junction development
locomotory behavior
blood vessel morphogenesis
axon extension
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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