CD16/CD32 Monoclonal Antibody (FRC-4G8) - FAQs

查看更多产品信息 CD16/CD32 Monoclonal Antibody (FRC-4G8) - FAQs (MFCR004)

2 个常见问题解答

我应该用哪些成分封闭用于流式细胞仪分析的细胞？

人们常使用与荧光抗体来源于同一种属的血清来进行封闭。如果没有使用二抗，这一成分通常为一抗；但如果使用二抗，这种成分就是二抗的宿主。用于直接标记的小鼠一抗时，Fc封闭试剂可以替代鼠血清。CD16 + CD32抗体（FRC-4G8）（货号MFCR004）是一个靶向免疫球蛋白gamma复合体Fc的低亲和力受体。

What should I use to block my cells for flow cytometry analysis?

Use serum from the same species as the host species of the secondary antibody for blocking. If the serum is not available, use from 2 to 5% BSA (Fraction V, defatted). If using only a primary antibody, such as directly-labeled mouse primary antibodies, a good blocking reagent is Fc block. CD16 + CD32 Antibody (FRC-4G8) (Cat. No. MFCR004 or MA5-16680) is a low-affinity receptor for the Fc region of immunoglobulin gamma complexes.

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