Human neutrophil Fc gamma receptor distribution and structure.
AuthorsFleit HB, Wright SD, Unkeless JC
JournalProc Natl Acad Sci U S A
PubMed ID6808506
'Fc receptors (FcR) for IgG on human cells were analyzed with two monoclonal antibodies, 3G8 and 4F7. Fab fragments of both hybridoma IgGs inhibited binding to neutrophils of soluble rabbit IgG complexes and sheep erythrocytes (E) coated with rabbit IgG. The number of sites for 3G8 Fab was 135,000 per ... More
Alternative membrane forms of Fc gamma RIII(CD16) on human natural killer cells and neutrophils. Cell type-specific expression of two genes that differ in single nucleotide substitutions.
AuthorsRavetch JV, Perussia B
JournalJ Exp Med
PubMed ID2526846
'A low affinity receptor for IgG immune complexes, Fc gamma RIII(CD16), is expressed on human NK cells as an integral membrane glycoprotein anchored through a transmembrane peptide; on polymorphonuclear neutrophils (PMN) the receptor is anchored through a phosphatidylinositol (PI) linkage. The protein on NK cells has a molecular mass 6-10 ... More
Activation of cloned human natural killer cells via Fc gamma RIII.
'The Fc gamma RIII (CD16) Ag on human NK cells involved in antibody-dependent cellular cytotoxicity has been demonstrated to be an important activation structure. The present studies were carried out to further characterize the functional role of the CD16 Ag and the mechanisms whereby cytotoxicity is activated by using human ... More
The PI-linked receptor FcRIII is released on stimulation of neutrophils.
AuthorsHuizinga TW, van der Schoot CE, Jost C, Klaassen R, Kleijer M, von dem Borne AE, Roos D, Tetteroo PA
JournalNature
PubMed ID2967437
'Human phagocytic cells express receptors for the constant (Fc) region of immunoglobulin G. Neutrophils carry Fc receptor II (FcRII; CDw32) and FcRIII (CD16) which both bind IgG-containing immune complexes, leading to phagocytosis of the complex and activation of the neutrophil. We find that patients with paroxysmal nocturnal haemoglobinuria (PNH) have ... More
Human Fc gamma RIII: cloning, expression, and identification of the chromosomal locus of two Fc receptors for IgG.
AuthorsPeltz GA, Grundy HO, Lebo RV, Yssel H, Barsh GS, Moore KW
JournalProc Natl Acad Sci U S A
PubMed ID2521732
A cDNA clone encoding a human receptor for the Fc portion of IgG (Fc gamma R), Fc gamma RIII or CD16, was isolated from a human leukocyte library by a transient expression-immunoselection procedure. This cDNA (pGP5) encodes a 46-kDa phosphatidylinositol-linked cell surface protein with CD16 determinants and affinity for human ... More
Functional and biochemical analysis of CD16 antigen on natural killer cells and granulocytes.
AuthorsLanier LL, Ruitenberg JJ, Phillips JH
JournalJ Immunol
PubMed ID2903193
CD16 Ag is associated with the low affinity FcR for IgG expressed on human NK cells and granulocytes. In this study, we demonstrate that NK cells specifically lyse murine anti-CD16 hybridoma cell lines, but do not lyse hybridomas against other cell surface differentiation Ag expressed on NK cells. Moreover, the ... More
The Fc gamma receptor of natural killer cells is a phospholipid-linked membrane protein.
AuthorsSimmons D, Seed B
JournalNature
PubMed ID2967436
Three types of receptor for the Fc (constant) region of human immunoglobulin G have been described; FcRI, a high-affinity (Ka approximately equal to 10(8) M-1) receptor expressed on monocytes; FcRII (CD32), a low-affinity (Ka approximately equal to 10(6) M-1) receptor expressed on B cells, granulocytes, macrophages and platelets; and FcRIII ... More
The Fc receptor for IgG on human natural killer cells: phenotypic, functional, and comparative studies with monoclonal antibodies.
AuthorsPerussia B, Trinchieri G, Jackson A, Warner NL, Faust J, Rumpold H, Kraft D, Lanier LL
JournalJ Immunol
PubMed ID6233371
We compare five monoclonal antibodies ( B73 .1, 3G8 , Leu- 11a , Leu- 11b , and VEP13 ) that react with natural killer (NK) cells and polymorphonuclear cells (PMN). We show that all of these antibodies are directed against and inhibit the functional properties of the receptor for the ... More