BLAST-2 [EBVCS], an early cell surface marker of human B cell activation, is superinduced by Epstein Barr virus.
AuthorsThorley-Lawson DA, Nadler LM, Bhan AK, Schooley RT
JournalJ Immunol
PubMed ID2984280
'Activation of human B cells by pokeweed mitogen (PWM), protein A, anti-IgM, or EBV infection results in the expression of a new surface antigen, termed BLAST-2 [EBVCS]. This marker appears before the cells undergo blast transformation as assessed by the initiation of DNA synthesis and expression of the BLAST-1 antigen. ... More
Interleukin 5 enhances interleukin 4-induced IgE production by normal human B cells. The role of soluble CD23 antigen.
AuthorsPène J, Rousset F, Brière F, Chrétien I, Wideman J, Bonnefoy JY, De Vries JE
JournalEur J Immunol
PubMed ID3260186
'Interleukin 4 (IL 4)-induced IgE production by peripheral blood lymphocytes and tonsil cells from normal donors was enhanced in a dose-dependent fashion by IL 5. IL 5 tested alone was not effective. The synergistic effects of IL 5 were most pronounced at suboptimal IL 4 concentrations, whereas at saturating IL ... More
Fc epsilon receptor, a specific differentiation marker transiently expressed on mature B cells before isotype switching.
AuthorsKikutani H, Suemura M, Owaki H, Nakamura H, Sato R, Yamasaki K, Barsumian EL, Hardy RR, Kishimoto T
JournalJ Exp Med
PubMed ID2945890
'The expression of Fc epsilon R on human lymphocytes was studied with the anti-Fc epsilon R mAbs. Fc epsilon R was expressed on most mu+,delta+ circulating B cells, whereas T cells did not express Fc epsilon R even in patients with hyper-IgE syndrome. B cells with gamma, alpha, or epsilon ... More
Human recombinant interleukin 4 induces Fc epsilon R2/CD23 on normal human monocytes.
AuthorsVercelli D, Jabara HH, Lee BW, Woodland N, Geha RS, Leung DY
JournalJ Exp Med
PubMed ID2965737
'rIL-4 (B cell stimulatory factor 1) induces the expression of Fc epsilon R2/CD23 on normal human monocytes (Mo). Fc epsilon R2/CD23 induction was detectable both by flow cytometry using anti-CD23 mAbs as well as soluble IgE, and by the immunoprecipitation with CD23-specific mAb or IgE of a 45-kD band from ... More
A B cell-specific differentiation antigen, CD23, is a receptor for IgE (Fc epsilon R) on lymphocytes.
AuthorsYukawa K, Kikutani H, Owaki H, Yamasaki K, Yokota A, Nakamura H, Barsumian EL, Hardy RR, Suemura M, Kishimoto T
JournalJ Immunol
PubMed ID2951441
Two independent L cell transformants expressing human lymphocyte Fc epsilon R were established by using cellular DNA from RPMI 8866 cells. The surface expression of the receptor was confirmed on the basis of the binding of a panel of anti-Fc epsilon R antibodies and its ability to bind IgE. Anti-CD23 ... More
The activation antigen BLAST-2, when shed, is an autocrine BCGF for normal and transformed B cells.
AuthorsSwendeman S, Thorley-Lawson DA
JournalEMBO J
PubMed ID3038527
A shed form of the membrane-bound B cell activation marker, BLAST-2 (Epstein-Barr virus cell surface, CD 23) was immune-affinity purified from Epstein-Barr virus-transformed lymphoblast conditioned medium. SDS-PAGE analysis revealed a complex of two polypeptides, mol. wts 25,000 and 12,000, here termed s-BLAST-2. We show that this complex, when purified to ... More
Molecular structure of human lymphocyte receptor for immunoglobulin E.
AuthorsKikutani H, Inui S, Sato R, Barsumian EL, Owaki H, Yamasaki K, Kaisho T, Uchibayashi N, Hardy RR, Hirano T
JournalCell
PubMed ID2877743
We have isolated and sequenced a cDNA clone encoding the human lymphocyte receptor for IgE (Fc epsilon R). The deduced protein sequence reveals that Fc epsilon R consists of 321 amino acids, without any signal sequence, and is oriented with its N-terminus on the cytoplasmic side and its C-terminus on ... More
Coordinated action of IgE and a B-cell-stimulatory factor on the CD23 receptor molecule up-regulates B-lymphocyte growth.
AuthorsGuy GR, Gordon J
JournalProc Natl Acad Sci U S A
PubMed ID2957693
The CD23 (BLAST-2) antigen, recently identified as the low-affinity IgE receptor of B lymphocytes, has also been implicated as the focus for growth-promoting signals delivered to activated B cells by a low molecular weight B-cell growth factor (BCGF). Here we show that IgE and BCGF can coordinate B-lymphocyte growth through ... More