Biochemical studies of the human thymocyte cell-surface antigens T6, T9 and T10.
AuthorsTerhorst C, van Agthoven A, LeClair K, Snow P, Reinherz E, Schlossman S
JournalCell
PubMed ID7013988
Three human thymic cell-surface antigens T6, T9 and T10, previously defined by monoclonal antibodies, were analyzed using immunoprecipitation techniques. The antigen T6 was found to be a 49,000 dalton glycoprotein, which is associated with beta 2-microglobulin, the small subunit (12,000 daltons) of the HLA-A, -B, and -C antigens. The target ... More
Discontinuous expression of a membrane antigen (HB-7) during B lymphocyte differentiation.
AuthorsTedder TF, Clement LT, Cooper MD
JournalTissue Antigens
PubMed ID6440308
We have examined the expression of a cell surface antigen by B lineage cells in human fetuses, newborns and adults using a newly produced monoclonal antibody, HB-7. The HB-7 antigen was found to be a protease sensitive 45,000 MW molecule that appeared to be the same molecule recognized by the ... More
Stages of B cell differentiation in human lymphoid tissue.
AuthorsBhan AK, Nadler LM, Stashenko P, McCluskey RT, Schlossman SF
JournalJ Exp Med
PubMed ID7024458
Monoclonal antibodies reactive with B cell-specific differentiation and other antigens were used to investigate stages of B cell maturation in human lymphoid tissue, using an immunoperoxidase technique on frozen tissue sections. Lymphoid follicles, which represent the major anatomic compartment of B cells, demonstrated cellular antigenic expressions that appear to reflect ... More
Discrete stages of human intrathymic differentiation: analysis of normal thymocytes and leukemic lymphoblasts of T-cell lineage.
AuthorsReinherz EL, Kung PC, Goldstein G, Levey RH, Schlossman SF
JournalProc Natl Acad Sci U S A
PubMed ID6966400
A series of monoclonal antibodies was used to define three discrete stages of human intrathymic T-cell differentiation. The earliest stage was confined to <10% of thymocytes, which were.reactive with both OKT9 and OKT10. Subsequently, approximately 70% of human thymocytes acquired a thymocyte-restricted antigen, OKT6, lost OKT9 antigen, and expressed reactivity ... More