Qualification of embryonal carcinoma 2102Ep as a reference for human embryonic stem cell research.
AuthorsJosephson R, Ording CJ, Liu Y, Shin S, Lakshmipathy U, Toumadje A, Love B, Chesnut JD, Andrews PW, Rao MS, Auerbach JM
JournalStem Cells
PubMed ID17284651
'As the number of human embryonic stem cell (hESC) lines increases, so does the need for systematic evaluation of each line''s characteristics and potential. Comparisons between lines are complicated by variations in culture conditions, feeders, spontaneous differentiation, and the absence of standardized assays. These difficulties, combined with the inability of ... More
DGCR8 is essential for microRNA biogenesis and silencing of embryonic stem cell self-renewal.
AuthorsWang Y, Medvid R, Melton C, Jaenisch R, Blelloch R,
JournalNat Genet
PubMed ID17259983
The molecular controls that govern the differentiation of embryonic stem (ES) cells remain poorly understood. DGCR8 is an RNA-binding protein that assists the RNase III enzyme Drosha in the processing of microRNAs (miRNAs), a subclass of small RNAs. Here we study the role of miRNAs in ES cell differentiation by ... More