ULTIMATE MOUSE ORF CLONE - FAQs

查看更多产品信息 ULTIMATE MOUSE ORF CLONE - FAQs (MORF01)

6 个常见问题解答

用于Ultimate ORF克隆的骨架载体名称是什么？它的主要特征及优点是什么？

Ultimate ORF克隆使用的骨架载体为pENTR221，它是一种Gateway入门载体。插入片段（一个以ATG起始并以TAG作为终止密码子的开放读码框）两侧为attL1及attL2位点。构建好的重组子可直接用于和带有attR1和attR2的目的载体（DEST载体）一起进行Gateway LR重组反应。它与N端带标签的目标载体兼容，但不与C端带标签的目标载体兼容。为了表达C端标签，需使用标签开启技术（Tag-On-Demand技术）或者将TAG终止密码子突变。我们提供定制化的表达tRNA抑制子的载体以代替我们之前出售的Tag-On-Demand腺病毒产品。如果您对此服务感兴趣，请发电子邮件至 custom.services@lifetech.com .

Ultimate ORF库与其它cDNA克隆的区别是什么？

我们提供的cDNA克隆包含Ultimate ORF克隆，全长人类cDNA克隆，MGC克隆及GeneStorm 直接表达克隆。Ultimate ORF克隆，全长人类cDNA克隆和GeneStorm直接表达克隆是我们内部生产的，而MGC克隆是从外部获得并由ThermoFisher Scientific分销的。Ultimate ORF克隆全部经过测序并保证在氨基酸水平100%匹配GenBank序列信息，而我们提供的其它cDNA克隆没有此担保。

我该选择哪种克隆：Ultimate ORF、全长人类cDNA，还是MGC？

如果您主要考虑的是质量和序列，我们推荐选择Ultimate ORF克隆。此外，这些克隆采用Gateway载体骨架，插入片段能方便地在多种宿主系统间穿梭。全长人类cDNA克隆和MGC克隆仅仅是5’和3’末端测过序的，但是全长人类cDNA克隆的优点是目的基因克隆在Gateway入门载体的骨架中。MGC克隆可提供人、小鼠和大鼠的；Ultimate ORF克隆可提供人和小鼠的，全长人类cDNA克隆只有人的。

What is the name of the backbone vector that is used for your Ultimate ORF clones? What are its main features and benefits?

The backbone vector that is used is pENTR221, which is a Gateway Entry vector. The insert (an open reading frame that begins with ATG and ends with a TAG stop codon) is flanked by attL1 and attL2 sites. The construct is ready for Gateway LR recombination with a DEST vector containing attR1 and attR2 sites. It is compatible with N-terminal tagged DEST vectors but not with C-terminal tagged DEST vectors. To express the C-terminal tag, the Tag-On-Demand technology would have to be used or the TAG stop codon would have to be mutated. In place of the Tag-On-Demand adenovirus product we sold previously, we offer the vector expressing the tRNA suppressor as a custom service. If you are interested in this service, please e-mail custom.services@lifetech.com.

How does your Ultimate ORF collection differ from your other cDNA clones?

The cDNA clones we offer are Ultimate ORF clones, Full-Length human cDNA clones, MGC clones, and GeneStorm Expression-Tested clones. The Ultimate ORF clones, Full-length human cDNA clones, and the GeneStorm Expression-Ready clones are made in-house, whereas the MGC clones are derived from external sources and distributed by Thermo Fisher Scientific. The Ultimate ORF clones are fully-sequenced and guaranteed to match GenBank sequence information 100% at the amino acid level, whereas the sequence of the other cDNA clones we offer is not guaranteed.

Which clone collection should I pick: Ultimate ORF, Full-length human cDNA, or MGC?

We recommend choosing the Ultimate ORF clones if quality and sequence are your main priority. Further, these clones are provided in a Gateway vector backbone, so it is easy to shuttle the insert into multiple host systems. The Full-length human cDNA clones and MGC clones are only 5´ and 3´ end-sequenced, but Full-length human cDNA clones offer the advantage of being in a Gateway Entry vector backbone. MGC clones are available for human, mouse, and rat species; Ultimate ORF clones are available for human and mouse species and Full-Length human cDNA clones are available only for human species.