Determination of in vivo protein phosphorylation in photosynthetic membranes.
AuthorsVainonen JP, Vener AV, Aro EM,
JournalMethods Mol Biol
PubMed ID19083170
Light- and redox-controlled reversible phosphorylation of thylakoid proteins regulates short- and long-term acclimation of plants to environmental cues. The major phosphoproteins in thylakoids belong to photosystem II and its light-harvesting antenna but phosphorylation of subunits of other thylakoid protein complexes has been detected as well. The detection methods include electrophoretic ... More