Dual luminescence-based reporter gene assay for luciferase and beta-galactosidase.
AuthorsMartin CS, Wight PA, Dobretsova A, Bronstein I,
JournalBiotechniques
PubMed ID8879594
'A unique combined luminescence assay for firefly (Photinus pyralis) luciferase and beta-galactosidase (beta-gal) reporter gene products is described. Luciferase and beta-gal activities are determined with the same aliquot of cell lysate prepared from cells contransfected with both reporter genes, thereby reducing manual labor and increasing experimental accuracy. With the Dual-Light ... More