Developmental shift in the relative percentages of lung fibroblast subsets: role of apoptosis postseptation.
AuthorsAwonusonu F,Srinivasan S,Strange J,Al-Jumaily W,Bruce MC
JournalThe American journal of physiology
PubMed ID10516228
Global metabolic profiling of infection by an oncogenic virus: KSHV induces and requires lipogenesis for survival of latent infection.
AuthorsDelgado T, Sanchez EL, Camarda R, Lagunoff M,
JournalPLoS Pathog
PubMed ID22916018
Like cancer cells, virally infected cells have dramatically altered metabolic requirements. We analyzed global metabolic changes induced by latent infection with an oncogenic virus, Kaposi's Sarcoma-associated herpesvirus (KSHV). KSHV is the etiologic agent of Kaposi's Sarcoma (KS), the most common tumor of AIDS patients. Approximately one-third of the nearly 200 ... More
Three-colour fluorescence immunohistochemistry reveals the diversity of cells staining for macrophage markers in murine spleen and liver.
AuthorsLloyd CM, Phillips AR, Cooper GJ, Dunbar PR,
JournalJ Immunol Methods
PubMed ID18367204
'Macrophages have traditionally been identified in murine tissues using a small range of markers, typically F4/80, CD68 and CD11b. However many studies have suggested that substantial heterogeneity exists in macrophage populations, and no single marker, nor even pair of markers, can necessarily identify all the populations. Further, many of the ... More
Lipid-dependent bidirectional traffic of apolipoprotein B in polarized enterocytes.
AuthorsMorel E, Demignot S, Chateau D, Chambaz J, Rousset M, Delers F
JournalMol Biol Cell
PubMed ID14565984
'Enterocytes are highly polarized cells that transfer nutrients across the intestinal epithelium from the apical to the basolateral pole. Apolipoprotein B (apoB) is a secretory protein that plays a key role in the transepithelial transport of dietary fatty acids as triacylglycerol. The evaluation of the control of apoB traffic by ... More
Functional analysis of FSP27 protein regions for lipid droplet localization, caspase-dependent apoptosis, and dimerization with CIDEA.
AuthorsLiu K, Zhou S, Kim JY, Tillison K, Majors D, Rearick D, Lee JH, Fernandez-Boyanapalli RF, Barricklow K, Houston MS, Smas CM,
JournalAm J Physiol Endocrinol Metab
PubMed ID19843876
'The adipocyte-specific protein FSP27, also known as CIDEC, is one of three cell death-inducing DFF45-like effector (CIDE) proteins. The first known function for CIDEs was promotion of apoptosis upon ectopic expression in mammalian cells. Recent studies in endogenous settings demonstrated key roles for CIDEs in energy metabolism. FSP27 is a ... More
Cholesterol esterification by host and parasite is essential for optimal proliferation of Toxoplasma gondii.
AuthorsSonda S, Ting LM, Novak S, Kim K, Maher JJ, Farese RV, Ernst JD
JournalJ Biol Chem
PubMed ID11457847
'Upon host cell invasion the apicomplexan parasite Toxoplasma gondii resides in a specialized compartment termed the parasitophorous vacuole that is derived from the host cell membrane but modified by the parasite. Despite the segregation of the parasitophorous vacuole from the host endocytic network, the intravacuolar parasite has been shown to ... More
Regulated localization of Rab18 to lipid droplets: effects of lipolytic stimulation and inhibition of lipid droplet catabolism.
AuthorsMartin S, Driessen K, Nixon SJ, Zerial M, Parton RG
JournalJ Biol Chem
PubMed ID16207721
'Rab GTPases are crucial regulators of membrane traffic. Here we have examined a possible association of Rab proteins with lipid droplets (LDs), neutral lipid-containing organelles surrounded by a phospholipid monolayer, also known as lipid bodies, which have been traditionally considered relatively inert storage organelles. Although we found close apposition between ... More
High density lipoprotein-mediated cholesterol uptake and targeting to lipid droplets in intact L-cell fibroblasts. A single- and multiphoton fluorescence approach.
AuthorsFrolov A, Petrescu A, Atshaves BP, So PT, Gratton E, Serrero G, Schroeder F
JournalJ Biol Chem
PubMed ID10777574
'Fluorescent sterols, dehydroergosterol and NBD-cholesterol, were used to examine high density lipoprotein-mediated cholesterol uptake and intracellular targeting in L-cell fibroblasts. The uptake, but not esterification or targeting to lipid droplets, of these sterols differed >100-fold, suggesting significant differences in uptake pathways. NBD-cholesterol uptake kinetics and lipoprotein specificity reflected high density ... More
Single-stage evaluation of serum lipoproteins by gel permeation using a specific fluorescent lipid probe.
AuthorsKnobler H, Fainaru M, Sklan D
JournalJ Chromatogr
PubMed ID3429561
Three probe flow cytometry of a human foam-cell forming macrophage.
AuthorsHassall DG
JournalCytometry
PubMed ID1526197
'A human cell line THP-1 was differentiated into macrophages expressing the scavenger receptor for uptake of modified lipoproteins. The cells were exposed to native low-density lipoprotein (n-LDL), acetylated-low-density lipoprotein (Ac-LDL), oxidised-LDL, or 25-OH cholesterol, leading to the accumulation of cholesteryl esters within the cells. Harvested macrophages were studied using three ... More
Autoantibodies to ribosomal P proteins penetrate into live hepatocytes and cause cellular dysfunction in culture.
AuthorsKoscec M, Koren E, Wolfson-Reichlin M, Fugate RD, Trieu E, Targoff IN, Reichlin M
JournalJ Immunol
PubMed ID9257871
'Abs to ribosomal P protein have been shown to bind a membrane form of the P0 38-kDa ribosomal phosphoprotein. This study shows that after affinity-purified Abs to ribosomal P proteins bind living HepG2 cells, they then penetrate these live cells and cause cellular dysfunction. Binding and penetration of anti-P Abs ... More
Modeling progression of fluorescent probes in bioinspired lignocellulosic assemblies.
AuthorsPaës G, Burr S, Saab MB, Molinari M, Aguié-Béghin V, Chabbert B,
Journal
PubMed ID23721261
'Progression of enzymes in lignocellulosic biomass is a crucial parameter in biorefinery processes, and it appears to be one of the limiting factors in optimizing lignocellulose degradation. In order to assay the importance of the chemical and structural features of the substrate matrix on enzyme mobility, we have designed bioinspired ... More
Factors underlying the variability of lipid droplet fluorescence in MA-10 Leydig tumor cells.
AuthorsGocze PM, Freeman DA
JournalCytometry
PubMed ID7835165
'Neutral lipids accumulate in cellular lipid droplets. These droplets vary remarkably in number and amount between cells. In the present studies, the variability in lipid content was quantified by comparing the coefficient of variation of fluorescence histograms of nile red lipid-stained cells to the variability of cell size or cell ... More
Fluorescent vital stains for complementary labelling of protoplasts from Trichoderma spp.
AuthorsHarman GE, Stasz TE
JournalStain Technol
PubMed ID2464211
'In this study several fluorescent vital stains were evaluated for their ability to provide complementary vital staining of protoplasts of Trichoderma spp. for selection of heterokaryons following protoplast fusion. Tetramethyl rhodamine isothiocyanate and fluorescein isothiocyanate were rejected because they stained only a small proportion of protoplasts. Fluorescein diacetate stained all ... More
Evidence for temperature-dependent conformational changes in the L-lactate dehydrogenase from Bacillus stearothermophilus.
AuthorsKotik M, Zuber H
JournalBiochemistry
PubMed ID1510965
'L-Lactate dehydrogenase from Bacillus stearothermophilus (BSLDH) has been shown to change its conformation in a temperature-dependent manner in the temperature range between 25 and 70 degrees C. To provide a more detailed understanding of this reversible structural reorganization of the tetrameric form of BSLDH, we have determined in the presence ... More
Green-light transilluminator for the detection without photodamage of proteins and DNA labeled with different fluorescent dyes.
AuthorsAlba FJ, Bermúdez A, Daban JR
JournalElectrophoresis
PubMed ID11258745
'The excitation spectra of Nile red and SYPRO red, two currently used dyes for the fluorescent staining of protein bands in sodium dodecyl sulfate (SDS)-polyacrylamide gels, show an excitation peak in the UV region and another in the visible region (maximum at about 550 nm). Ethidium bromide and other intercalating ... More
Cholesteryl ester accumulation in macrophages treated with oxidized low density lipoprotein.
AuthorsRyu BH, Mao FW, Lou P, Gutman RL, Greenspan P
JournalBiosci Biotechnol Biochem
PubMed ID8520107
'The ability of CuSO4- and hypochlorite-oxidized LDL to promote cholesterol accumulation in macrophages was examined. Both CuSO4- and hypochlorite-oxidized LDL were rapidly metabolized by mouse peritoneal macrophages to a level approximately 10 times that observed for native LDL and both modified lipoproteins increased the accumulation of unesterified cholesterol. However when ... More
Nile red staining of lysosomal phospholipid inclusions.
AuthorsBrown WJ, Sullivan TR, Greenspan P
JournalHistochemistry
PubMed ID1319977
'We have employed the fluorescent dye nile red to distinguish between normal cells and cells containing lysosomal accumulations of phospholipids. When fibroblasts from an individual with a genetic deficiency in lysosomal sphingomyelinase activity (Niemann-Pick disease) were stained with nile red and visualized by fluorescence microscopy, orange-colored inclusions were observed throughout ... More
Use of Nile red stain in the detection of cholesteryl ester accumulation in acid lipase-deficient fibroblasts.
AuthorsBrown WJ, Warfel J, Greenspan P
JournalArch Pathol Lab Med
PubMed ID3345127
'The fluorescent hydrophobic probe Nile red was used to distinguish between normal human fibroblasts and fibroblasts from individuals with a genetic deficiency in lysosomal acid lipase activity (Wolman''s disease and cholesteryl ester storage disease). The fluorescence of Nile red-stained cultured mutant cells, indicative of neutral lipid accumulation, was intense when ... More
Ligand-mediated changes in the tryptophan synthase indole tunnel probed by nile red fluorescence with wild type, mutant, and chemically modified enzymes.
AuthorsRuvinov SB, Yang XJ, Parris KD, Banik U, Ahmed SA, Miles EW, Sackett DL
JournalJ Biol Chem
PubMed ID7890774
'The bacterial tryptophan synthase alpha 2 beta 2 complex contains an unusual structural feature: an intramolecular tunnel that channels indole from the active site of the alpha subunit to the active site of the beta subunit 25 A away. Here we investigate the role of the tunnel in communication between ... More
Differentiation of A31T6 proadipocytes to adipocytes: a flow cytometric analysis.
AuthorsSmyth MJ, Wharton W
JournalExp Cell Res
PubMed ID1735459
'A flow cytometric assay has been developed which provides precise, quantitative information on the accumulation of cytoplasmic triglycerides in individual A31T6 proadipocytes as they differentiate into adipocytes. The opportunity to measure multiple optical parameters on a cell-by-cell basis has enabled us to monitor phenotypic aspects of differentiation with a greater ... More
Bacterial DNA segregation by dynamic SopA polymers.
AuthorsLim GE, Derman AI, Pogliano J
JournalProc Natl Acad Sci U S A
PubMed ID16306264
'Many bacterial plasmids and chromosomes rely on ParA ATPases for proper positioning within the cell and for efficient segregation to daughter cells. Here we demonstrate that the F-plasmid-partitioning protein SopA polymerizes into filaments in an ATP-dependent manner in vitro, and that the filaments elongate at a rate that is similar ... More
Use of the hydrophobic probe Nile red for the fluorescent staining of protein bands in sodium dodecyl sulfate-polyacrylamide gels.
AuthorsDaban JR, Bartolomé S, Samsó M
JournalAnal Biochem
PubMed ID1725949
'In a previous work (J.-R. Daban, M. Samsó, and S. Bartolomé, Anal. Biochem. 199, 162-168, 1991) we observed that, in the presence of the detergent sodium dodecyl sulfate (SDS), diverse types of proteins produced a high increase in the fluorescence intensity of the hydrophobic probe 9-diethylamino-5H-benzo[alpha]-phenoxazine-5-one (Nile red). This enhancement ... More
A caveolin dominant negative mutant associates with lipid bodies and induces intracellular cholesterol imbalance.
AuthorsPol A, Luetterforst R, Lindsay M, Heino S, Ikonen E, Parton RG
JournalJ Cell Biol
PubMed ID11238460
'Recent studies have indicated a role for caveolin in regulating cholesterol-dependent signaling events. In the present study we have analyzed the role of caveolins in intracellular cholesterol cycling using a dominant negative caveolin mutant. The mutant caveolin protein, cav-3(DGV), specifically associates with the membrane surrounding large lipid droplets. These structures ... More
Transient exposure of hydrophobic surface in the photoactive yellow protein monitored with Nile Red.
AuthorsHendriks J, Gensch T, Hviid L, van Der Horst MA, Hellingwerf KJ, van Thor JJ
JournalBiophys J
PubMed ID11867475
'In this study we have investigated binding of the fluorescent hydrophobicity probe Nile Red to the photoactive yellow protein (PYP), to characterize the exposure and accessibility of hydrophobic surface upon formation of the signaling state of this photoreceptor protein. Binding of Nile Red, reflected by a large blue shift and ... More
Use of nile red as a fluorescent probe for the study of the hydrophobic properties of protein-sodium dodecyl sulfate complexes in solution.
AuthorsDaban JR, Samsó M, Bartolomé S
JournalAnal Biochem
PubMed ID1812781
'Our results show that the noncovalent dye 9-diethylamino-5H-benzo[alpha]phenoxazine-5-one (Nile red) can be used as a fluorescent probe to study the hydrophobic properties of proteins associated with the anionic detergent sodium dodecyl sulfate (SDS). Nile red can interact with both SDS micelles and protein-SDS complexes. The enhancement of Nile red fluorescence ... More
Nile red labeling of single living cells for contour delineation to quantify and evaluate the distribution of rhodamine 123 with fluorescence image cytometry.
AuthorsCanitrot Y, Lautier D, Lahmy S, Vigo J, Viallet P, Salmon JM
JournalJ Histochem Cytochem
PubMed ID8245427
'Simultaneous study of intracellular quantification and distribution of fluorescent probes is difficult when cell staining is not homogeneous. This occurs after mitochondrial staining with rhodamine 123 (R123). Classical techniques for evaluation of intracellular R123 fluorescence, such as flow cytometry, are based on measurement of the global fluorescence intensity but do ... More
Fluorescent high-content imaging allows the discrimination and quantitation of E-LDL-induced lipid droplets and Ox-LDL-generated phospholipidosis in human macrophages.
AuthorsGrandl M, Schmitz G,
JournalCytometry A
PubMed ID20014301
'Macrophage foam cells formed during uptake of atherogenic lipoproteins are a hallmark of atherosclerotic lesion development. In this study, human macrophages were incubated with two prototypic atherogenic LDL modifications enzymatically degraded LDL (E-LDL) and oxidized LDL (Ox-LDL) prepared from the same donor LDL. To detect differences in macrophage lipid storage, ... More
Application of the standard addition method for the absolute quantification of neutral lipids in microalgae using Nile red.
AuthorsBertozzini E, Galluzzi L, Penna A, Magnani M,
JournalJ Microbiol Methods
PubMed ID21767582
'Microalgae are considered one of the best candidates for biofuel production due to their high content in neutral lipids, therefore, an accurate quantification of these lipids in microalgae is fundamental for the identification of the better candidates as biodiesel source. Nile red is a fluorescent dye widely employed for the ... More
Characterization of six textile dyes as fluorescent stains for flow cytometry.
AuthorsSimmons DM, Mercer AV, Hallas G, Dyson JE
JournalJ Histochem Cytochem
PubMed ID1688448
'Few fluorescent stains specific for cell constituents other than DNA are available. To assess their potential use as fluorescent stains for flow cytometry, the cell staining specificity of 55 compounds, originally synthesized for use as textile dyes and fluorescent brighteners, was explored and their excitation and emission wavebands determined. From ... More
Bone morphogenetic proteins inhibit adipocyte differentiation by bone marrow stromal cells.
AuthorsGimble JM, Morgan C, Kelly K, Wu X, Dandapani V, Wang CS, Rosen V
JournalJ Cell Biochem
PubMed ID7593260
'The bone morphogenetic proteins were originally identified based on their ability to induce ectopic bone formation in vivo and have since been identified as members of the transforming growth factor-beta gene superfamily. It has been well established that the bone morphogenetic cytokines enhance osteogenic activity in bone marrow stromal cells ... More
Induction of endocytic vesicles by exogenous C(6)-ceramide.
AuthorsLi R, Blanchette-Mackie EJ, Ladisch S
JournalJ Biol Chem
PubMed ID10409665
'Ceramide is a newly discovered second messenger that has been shown to cause cell growth arrest and apoptosis. Here, we present evidence that exogenously added C(6)-ceramide induces enlargement of late endosomes and lysosomes. 10 microM C(6)-ceramide caused the formation of numerous vesicles of varying sizes (2-10 micrometers) in fibroblasts (3T3-L1 ... More
Application of Nile red, a fluorescent hydrophobic probe, for the detection of neutral lipid deposits in tissue sections: comparison with oil red O.
AuthorsFowler SD, Greenspan P
JournalJ Histochem Cytochem
PubMed ID4020099
'Nile red is a phenoxazone dye that fluoresces intensely, and in varying color, in organic solvents and hydrophobic lipids. However, the fluorescence is fully quenched in water. The dye acts, therefore, as a fluorescent hydrophobic probe. We utilized this novel property of nile red to develop a sensitive fluorescent histochemical ... More
High-speed fluorescence detection of explosives-like vapors
AuthorsAlbert KJ, Walt DR
JournalAnal Chem
PubMed ID10815950
'In this paper, we report on the preparation of novel cross-reactive optical microsensors for high-speed detection of low-level explosives and explosives-like vapors. Porous silica microspheres with an incorporated environmentally sensitive fluorescent dye are employed in high-density sensor arrays to monitor fluorescence changes during nitroaromatic compound (NAC) vapor exposure. The porous ... More
Crystal engineering: from structure to function.
AuthorsHollingsworth MD
JournalScience
PubMed ID11923527
'Modern crystal engineering has emerged as a rich discipline whose success requires an iterative process of synthesis, crystallography, crystal structure analysis, and computational methods. By focusing on the molecular recognition events during nucleation and growth, chemists have uncovered new ways of controlling the internal structure and symmetry of crystals and ... More
Lipid signals detected by NMR proton spectroscopy of whole cells are not correlated to lipid droplets evidenced by the Nile red staining.
'Nile red staining was used to detect lipid droplets in the K562 cell line sensitive and resistant to adriamycin and their resistance-reversing counterparts. The staining obtained was compared to the intensity of lipid signal detected in proton nuclear magnetic resonance spectra. From the four cell lines used, a lack of ... More
Identification of hypoxic and oxygenated tumor subpopulations with enzyme and fluorescent probes.
AuthorsFreitas I, Pontiggia P
JournalJ Photochem Photobiol B
PubMed ID2107296
Extrinsic fluorescent dyes as tools for protein characterization.
AuthorsHawe A, Sutter M, Jiskoot W,
JournalPharm Res
PubMed ID18172579
'Noncovalent, extrinsic fluorescent dyes are applied in various fields of protein analysis, e.g. to characterize folding intermediates, measure surface hydrophobicity, and detect aggregation or fibrillation. The main underlying mechanisms, which explain the fluorescence properties of many extrinsic dyes, are solvent relaxation processes and (twisted) intramolecular charge transfer reactions, which are ... More
Assessment of Escherichia coli B with enhanced permeability to fluorochromes for flow cytometric assays of bacterial cell function.
AuthorsHerrera G, Martinez A, Blanco M, O'Connor JE
JournalCytometry
PubMed ID12357461
'BACKGROUND: Flow cytometry has become a choice methodology for microbiological research. However, functional cytometric assays in live bacteria are still limited. This is due, in part, to the cell wall impairing penetration of vital dyes in bacteria, thus imposing permeabilization procedures. These manipulations may affect cell physiology, provoke cell aggregation ... More
Phosphate starvation in fungi induces the replacement of phosphatidylcholine with the phosphorus-free betaine lipid diacylglyceryl-n,n,n-trimethylhomoserine.
'Diacylglyceryl-N,N,N-trimethylhomoserine (DGTS) is a phosphorus-free betaine-lipid analog of phosphatidylcholine (PtdCho) synthesized by many soil bacteria, algae, and nonvascular plants. Synthesis of DGTS and other phosphorus-free lipids in bacteria occurs in response to phosphorus (P) deprivation and results in the replacement of phospholipids by nonphosphorous lipids. The genes encoding DGTS biosynthetic ... More
Ca2+ signal transduction related to neutral lipid synthesis in an oil-producing green alga Chlorella sp. C2.
AuthorsChen H, Zhang Y, He C, Wang Q,
Journal
PubMed ID24449653
'Changes in the cytosolic Ca(2+) levels and the role of Ca(2+) signal transduction in neutral lipid synthesis in Chlorella sp. C2 under nitrogen starvation conditions were investigated. The results detected by using the scanning ion-selective electrode technique demonstrate that nitrogen starvation induced significant Ca(2+) influx across the plasma membrane into ... More
D-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol alters cellular cholesterol homeostasis by modulating the endosome lipid domains.
AuthorsMakino A, Ishii K, Murate M, Hayakawa T, Suzuki Y, Suzuki M, Ito K, Fujisawa T, Matsuo H, Ishitsuka R, Kobayashi T
JournalBiochemistry
PubMed ID16584188
'D-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol (D-PDMP) is a frequently used inhibitor of glycosphingolipid biosynthesis. However, some interesting characteristics of D-PDMP cannot be explained by the inhibition of glycolipid synthesis alone. In the present study, we showed that d-PDMP inhibits the activation of lysosomal acid lipase by late endosome/lysosome specific lipid, bis(monoacylglycero)phosphate (also called as ... More
Photosensitization, uptake, and retention of phenoxazine Nile blue derivatives in human bladder carcinoma cells.
'The overall goal of our research is to develop effective new photosensitizers for tumor-selective photodynamic therapy. Phenoxazine dyes, including several Nile blue analogues, are known to localize selectively in animal tumors. Structural modifications yielded several series of analogues with substantially higher 1O2 yields and different photochemical and physicochemical properties. This ... More
Attachment of steroidogenic lipid droplets to intermediate filaments in adrenal cells.
AuthorsAlmahbobi G, Williams LJ, Hall PF
JournalJ Cell Sci
PubMed ID1629251
'Light microscopy of living and extracted adrenal cells (Y-1 mouse adrenal tumour cells and cultured bovine fasciculata cells), using Nomarski optics and fluorescence with nile red to stain lipid, revealed in both cell types that lipid droplets remain attached to intermediate filaments when the cells are extracted to prepare these ... More
Development of microalga Scenedesmus dimorphus mutant with higher lipid content by radiation breeding.
AuthorsChoi JI, Yoon M, Joe M, Park H, Lee SG, Han SJ, Lee PC,
Journal
PubMed ID24871276
'In this study, a high lipid-accumulating mutant strain of the microalgae Scenedesmus dimorphus was developed via radiation breeding. To induce mutant strain, S. dimorphus was gamma-irradiated at doses from 100 to 800 Gy, and then a mutant (Sd-Pm210) with 25 % increased lipid content was selected using Nile red staining methodology. Sd-Pm210 ... More
Nile Red binding to HepG2 cells: an improved assay for in vitro studies of hepatosteatosis.
AuthorsMcMillian MK, Grant ER, Zhong Z, Parker JB, Li L, Zivin RA, Burczynski ME, Johnson MD
JournalIn Vitr Mol Toxicol
PubMed ID11846991
'Nile Red is a fluorescent dye used extensively to study fat accumulation in many types of cells; unfortunately protocols that work well for most cells are not effective for studying drug-induced lipid accumulation in cultured liver cells and hepatocyte-derived cell lines. Using human hepatoma (HepG2) cells, we have developed a ... More
Bimodal distribution and fluorescence response of environment-sensitive probes in lipid bilayers.
AuthorsKlymchenko AS, Duportail G, Demchenko AP, Mély Y
JournalBiophys J
PubMed ID15111409
'A remarkable heterogeneity is often observed in the spectroscopic properties of environment-sensitive fluorescence probes in phospholipid bilayers. To explain its origin, we provided a detailed investigation of the fluorescence excitation and emission spectra of 4''-dimethylamino-3-hydroxyflavone (probe F) in bilayer vesicles with the variations of fatty acid composition, polar heads, temperature, ... More
Association of stomatin with lipid bodies.
AuthorsUmlauf E, Csaszar E, Moertelmaier M, Schuetz GJ, Parton RG, Prohaska R
JournalJ Biol Chem
PubMed ID15024010
'The oligomeric lipid raft-associated integral protein stomatin normally localizes to the plasma membrane and the late endosomal compartment. Similar to the caveolins, it is targeted to lipid bodies (LBs) on overexpression. Endogenous stomatin also associates with LBs to a small extent. Green fluorescent protein-tagged stomatin (StomGFP) and the dominant-negative caveolin-3 ... More
A chemical-detecting system based on a cross-reactive optical sensor array.
AuthorsDickinson TA, White J, Kauer JS, Walt DR
JournalNature
PubMed ID8751439
'The vertebrate olfactory system has long been recognized for its extraordinary sensitivity and selectivity for odours. Chemical sensors have been developed recently that are based on analogous distributed sensing properties, but although an association between artificial devices and the olfactory system has been made explicit in some previous studies, none ... More
Spectrofluorometric studies of the lipid probe, nile red.
AuthorsGreenspan P, Fowler SD
JournalJ Lipid Res
PubMed ID4031658
'We found that the dye nile red, 9-diethylamino-5H-benzo[alpha]phenoxazine-5-one, can be applied as a fluorescent vital stain for the detection of intracellular lipid droplets by fluorescence microscopy and flow cytofluorometry (J. Cell. Biol. 1985. 100: 965-973). To understand the selectivity of the staining, we examined the fluorescence properties of nile red ... More
Novel instrumentation and biomedical applications of very near infrared fluorescence.
AuthorsBrown MB, Edmonds TE, Miller JN, Riley DP, Seare NJ
JournalAnalyst
PubMed ID8494173
'A high wavelength fluorescent probe, Nile Red, was added to four proteins, viz., bovine albumin, alpha 1-acid glycoprotein, beta-lactoglobulin and ovomucoid. Nile Red showed an enhancement in fluorescence and a shift in emission wavelength, suggesting it was bonding hydrophobically to these proteins. Drug displacement of Nile Red from alpha 1-acid ... More
IGF-I is a mitogen involved in differentiation-related gene expression in fetal rat brown adipocytes.
AuthorsLorenzo M, Valverde AM, Teruel T, Benito M
JournalJ Cell Biol
PubMed ID8253851
'Fetal rat brown adipocytes at time zero of culture constitute a population of cells of broad spectrum, as estimated by cell size, endogenous fluorescence and lipid content, and show an intrinsic mitogenic competence. They express constitutively early growth-related genes such as c-myc, c-fos, and beta-actin, tissue specific-genes such as the ... More
Detection of human leukemia cells with multidrug-resistance phenotype using multilabeling with fluorescent dyes.
AuthorsLautier D, Lahmy S, Canitrot Y, Vigo J, Viallet P, Salmon JM
JournalAnticancer Res
PubMed ID8239535
'Reduced accumulation of multiple drugs is a characteristic of cells overexpressing P-glycoprotein. This phenotype is referred to as multidrug-resistance (MDR). A protocol based on reduced accumulation of fluorescent dyes is proposed for discriminating MDR cells in cell populations. The combination of three fluorescent dyes, Hoechst 33342, rhodamine 123 and Nile ... More
High-throughput fluorescence-activated cell sorting for lipid hyperaccumulating Chlamydomonas reinhardtii mutants.
AuthorsXie B, Stessman D, Hart JH, Dong H, Wang Y, Wright DA, Nikolau BJ, Spalding MH, Halverson LJ,
Journal
PubMed ID24702864
'The genetically tractable microalga Chlamydomonas reinhardtii has many advantages as a model for renewable bioproducts and/or biofuels production. However, one limitation of C. reinhardtii is its relatively low-lipid content compared with some other algal species. To overcome this limitation, we combined ethane methyl sulfonate mutagenesis with fluorescence-activated cell sorting (FACS) of ... More
Application of nile blue and nile red, two fluorescent probes, for detection of lipid droplets in human skeletal muscle.
AuthorsBonilla E, Prelle A
JournalJ Histochem Cytochem
PubMed ID3559182
'Using frozen sections from human muscle biopsies, we assessed the value of Nile blue and Nile red, two fluorescent probes, as stains for lipid droplets in normal and pathological skeletal muscle fibers. In normal muscle, lipid storage disorders, and mitochondrial myopathies, Nile blue stained the lipid droplets as yellow-gold fluorescent ... More
Esterified Cholesterol Is Highly Localized to Bruch's Membrane, as Revealed by Lipid Histochemistry in Wholemounts of Human Choroid.
AuthorsRudolf M, Curcio CA,
JournalJ Histochem Cytochem
PubMed ID19365091
'Accumulation of neutral lipids in Bruch''s membrane (BrM) is a major age change in human retina and contributes to the formation of extracellular lesions associated with age-related macular degeneration. We developed a BrM-choroid wholemounting technique suitable for reliable staining and evaluated different fluorescent lipid dyes for topographic semiquantitative analysis of ... More
Photoinduced electron transfer studies of Nile red in the presence of TiO2 colloidal nanoparticles.
AuthorsAnandan S, Yoon M
JournalSpectrochim Acta A Mol Biomol Spectrosc
PubMed ID15036099
'Photoinduced electron transfers between Nile red (NR) with TiO2 colloidal nanoparticles are studied using picosecond transient absorption and time resolved fluorescence spectroscopy. The dynamics of electron transfer from the dye molecule to the semiconductor were understood from the transient, and also the formation of conduction band electron and Nile red ... More
Interactions of molecular probes with living cells and tissues. Part 1. Some general mechanistic proposals, making use of a simplistic Chinese box model.
AuthorsHorobin RW, Rashid F
JournalHistochemistry
PubMed ID2358379
'A simple and generalised model-termed the simplistic Chinese box [SCB] model-for the interaction of molecular probes with living systems is described. The SCB model includes the following assumptions. That living systems may be considered as built from biologically defined boxes, e.g. whole cell, nucleus, nucleoli. That movement of molecular probes ... More
Direct blotting, sequencing and immunodetection of proteins after five-minute staining of SDS and SDS-treated IEF gels with Nile red.
AuthorsBermudez A, Daban JR, Garcia JR, Mendez E
JournalBiotechniques
PubMed ID8024781
'The non-covalent dye Nile red allows the fast and simple fluorescent staining of protein bands in sodium dodecyl sulfate (SDS)-polyacrylamide gels. This procedure has been extended to polyacrylamide isoelectric focusing gels that do not contain SDS. Unlike the current methods using Coomassie blue or silver for gel staining, Nile red ... More
Tricyclic antidepressant-induced lipidosis in human peripheral monocytes in vitro, as well as in a monocyte-derived cell line, as monitored by spectrofluorimetry and flow cytometry after staining with Nile red.
AuthorsXia Z, Appelkvist EL, DePierre JW, Nässberger L
JournalBiochem Pharmacol
PubMed ID9260880
'Human mono- and lymphocytes from peripheral blood and the monoblastoid cell line U-937 were used in this in vitro study of drug-induced lipidosis. Mono- and lymphocytes were exposed for 4 days to three different tricyclic antidepressants (TCAs), imipramine (25 microM), clomipramine (10 microM) and citalopram (80 microM). The lipophilic fluorophore ... More
Nondestructive detection of gangliosides with lipophilic fluorochromes and their employment for preparative high-performance thin-layer chromatography.
AuthorsMüthing J, Heitmann D
JournalAnal Biochem
PubMed ID8434781
'A simple and effective procedure for the isolation and purification of gangliosides by preparative thin-layer chromatography is described. The method is based on nondestructive visualization of gangliosides on silica gel-precoated thin-layer chromatography plates by staining with uncharged lipophilic fluorochromes. Fluorescent dyes were added in low concentrations into the mobile phase ... More
Genome-wide RNAi analysis of Caenorhabditis elegans fat regulatory genes.
'Regulation of body fat storage involves signalling between centres that regulate feeding in the brain and sites of fat storage and use in the body. Here we describe an assay for analysing fat storage and mobilization in living Caenorhabditis elegans. By using RNA-mediated interference (RNAi) to disrupt the expression of ... More
Multiple-probe analysis of folding and unfolding pathways of human serum albumin. Evidence for a framework mechanism of folding.
AuthorsSantra MK, Banerjee A, Krishnakumar SS, Rahaman O, Panda D
JournalEur J Biochem
PubMed ID15096218
'The changes in the far-UV CD signal, intrinsic tryptophan fluorescence and bilirubin absorbance showed that the guanidine hydrochloride (GdnHCl)-induced unfolding of a multidomain protein, human serum albumin (HSA), followed a two-state process. However, using environment sensitive Nile red fluorescence, the unfolding and folding pathways of HSA were found to follow ... More
Probing solvent fractionation in electrospray droplets with laser-induced fluorescence of a solvatochromic dye
AuthorsZhou S, Cook KD
JournalAnal Chem
PubMed ID10739199
'Laser-induced fluorescence spectroscopy is used to profile solvent fractionation in an electrospray plume containing Nile Red, a solvatochromic dye (i.e., a dye for which spectral features are sensitive to solvent polarity). The results confirm gradual enrichment of the less volatile component in spray droplets as a result of solvent evaporation. ... More
Oil production towards biofuel from autotrophic microalgae semicontinuous cultivations monitorized by flow cytometry.
Authorsda Silva TL, Reis A, Medeiros R, Oliveira AC, Gouveia L,
JournalAppl Biochem Biotechnol
PubMed ID19067244
'Two microalgae species (Scenedesmus obliquus and Neochloris oleoabundans) were cultivated in closed sleeve photobioreactors in order to select the best oil producer for further large-scale open raceway pond cultivations, aiming at biofuel production. Scenedesmus obliquus reached a higher maximum biomass concentration (1.41 g l(-1)) with a lower lipid content (12.8% ... More
Detection of five nanograms of protein by two-minute nile red staining of unfixed SDS gels.
AuthorsAlba FJ, Bermudez A, Bartolome S, Daban JR
JournalBiotechniques
PubMed ID8891212
Nile blue sulphate and brilliant cresyl blue in fluorescence methods for the histochemical demonstration of lipids.
AuthorsBozzo L, Vidal Bde C
JournalAnn Histochim
PubMed ID5717661
Foam cells and atherogenesis.
AuthorsFowler SD, Mayer EP, Greenspan P
JournalAnn N Y Acad Sci
PubMed ID3907470
Improved cytochemical method for detecting Jordans' bodies in neutral lipid storage diseases.
AuthorsTavian D, Colombo R
JournalJ Clin Pathol
PubMed ID17293389
Microsporidian spore invasion tubes as revealed by fluorescent probes.
AuthorsWeidner E, Manale SB, Halonen SK, Lynn JW
JournalBiol Bull
PubMed ID7811808
A high throughput Nile red method for quantitative measurement of neutral lipids in microalgae.
AuthorsChen W, Zhang C, Song L, Sommerfeld M, Hu Q,
JournalJ Microbiol Methods
PubMed ID19162091
Isolation of high neutral lipid-containing microalgae is key to the commercial success of microalgae-based biofuel production. The Nile red fluorescence method has been successfully applied to the determination of lipids in certain microalgae, but has been unsuccessful in many others, particularly those with thick, rigid cell walls that prevent the ... More
The life of lipid droplets.
AuthorsWalther TC, Farese RV,
JournalBiochim Biophys Acta
PubMed ID19041421
Lipid droplets are the least characterized of cellular organelles. Long considered simple lipid storage depots, these dynamic and remarkable organelles have recently been implicated in many biological processes, and we are only now beginning to gain insights into their fascinating lives in cells. Here we examine what we know of ... More
Nile red simultaneous staining of intracellular lipids and membrane network in human muscle cultures.
AuthorsSantilli I, Prelle A, Geremia L, Scarlato G, Meola G
JournalBasic Appl Histochem
PubMed ID2730516
We have applied a new fluorescent probe, Nile red, on normal and pathological human muscle derived cultures and compared the results with corresponding human muscle sections. In normal human muscle cultures, Nile red strain has proved useful for visualization of both intracellular lipids and membrane network. Similar patterns have been ... More
Photophysics of Nile red in solution: steady state spectroscopy.
AuthorsGhoneim N
JournalSpectrochim Acta A Mol Biomol Spectrosc
PubMed ID10809076
Spectroscopic properties of Nile red (NR) in organic solvents, binary solvent mixtures have been studied. Remarkable shifts in the emission band positions have been observed as a function of the polarity of the medium. In solvent mixtures, these shifts can be explained by the process of specific solvation known as ... More
Immobilization of DNA via oligonucleotides containing an aldehyde or carboxylic acid group at the 5' terminus.
AuthorsKremsky JN, Wooters JL, Dougherty JP, Meyers RE, Collins M, Brown EL
JournalNucleic Acids Res
PubMed ID3562241
A general method for the immobilization of DNA through its 5'-end has been developed. A synthetic oligonucleotide, modified at its 5'-end with an aldehyde or carboxylic acid, was attached to latex microspheres containing hydrazide residues. Using T4 polynucleotide ligase and an oligonucleotide splint, a single stranded 98mer was efficiently joined ... More
Three-dimensional imaging of single molecules solvated in pores of poly(acrylamide) gels.
AuthorsDickson RM, Norris DJ, Tzeng YL, Moerner WE
JournalScience
PubMed ID8875935
Individual fluorescent molecules and individual singly labeled proteins were observed in the water-filled pores of poly(acrylamide) gels by far-field microscopy. Brownian motion was markedly reduced by the gel framework, thus enabling extended study of single fluorophores in aqueous environments. A highly axially dependent laser field was used both to excite ... More
Monitoring biophysical properties of lipid membranes by environment-sensitive fluorescent probes.
AuthorsDemchenko AP, Mély Y, Duportail G, Klymchenko AS,
JournalBiophys J
PubMed ID19413953
We review the main trends in the development of fluorescence probes to obtain information about the structure, dynamics, and interactions in biomembranes. These probes are efficient for studying the microscopic analogs of viscosity, polarity, and hydration, as well as the molecular order, environment relaxation, and electrostatic potentials at the sites ... More
DAF-16 target genes that control C. elegans life-span and metabolism.
AuthorsLee SS, Kennedy S, Tolonen AC, Ruvkun G
JournalScience
PubMed ID12690206
Signaling from the DAF-2/insulin receptor to the DAF-16/FOXO transcription factor controls longevity, metabolism, and development in disparate phyla. To identify genes that mediate the conserved biological outputs of daf-2/insulin-like signaling, we used comparative genomics to identify 17 orthologous genes from Caenorhabditis and Drosophila, each of which bears a DAF-16 binding ... More
Spectrofluorometric studies of nile red treated native and oxidized low density lipoprotein.
AuthorsGreenspan P, Lou P
JournalInt J Biochem
PubMed ID8365550
1. The excitation and emission maxima of nile red in the presence of LDL were found to be 526 and 587 nm, respectively. Oxidation of LDL for 16 hr in the presence of CuSO4 resulted in significant spectral shifts to longer wavelengths in both the excitation and emission spectra. 2. ... More
Fluorescence resonance energy transfer in a novel two-photon absorbing system.
AuthorsBrousmiche DW, Serin JM, Fréchet JM, He GS, Lin TC, Chung SJ, Prasad PN
JournalJ Am Chem Soc
PubMed ID12568583
A novel fluorescence resonance energy transfer (FRET) system containing a two-photon absorbing dye and a nile red chromophore has been synthesized. Upon two-photon excitation by laser at 815 nm this molecule displays efficient energy transfer from the two-photon absorbing dye to the nile red moiety, with an 8-fold increase in ... More
Nile red as a polarity-sensitive fluorescent probe of hydrophobic protein surfaces.
AuthorsSackett DL, Wolff J
JournalAnal Biochem
PubMed ID3442318
Nile red is an uncharged hydrophobic molecule whose fluorescence is strongly influenced by the polarity of its environment. It interacts with many, but not all, native proteins, including beta-lactoglobulin, kappa-casein, and albumin, with a wide range of spectral changes for different proteins. It detects the exposure or formation of new ... More
Fluorescent labeling of proteins with nile red and 2-methoxy-2,4-diphenyl-3(2H)-furanone: physicochemical basis and application to the rapid staining of sodium dodecyl sulfate polyacrylamide gels and Western blots.
AuthorsDaban JR
JournalElectrophoresis
PubMed ID11332755
The fluorescent hydrophobic dye Nile red allows the rapid, sensitive, and general staining of proteins in sodium dodecyl sulfate (SDS)-polyacrylamide gels. Nile red staining does not preclude further electroblotting of protein bands onto polyvinylidene difluoride (PVDF) membranes. The resulting Western blot can be stained with the covalent fluorescent dye 2-methoxy-2,4-diphenyl-3(2H)-furanone ... More
Solvatochromism of Nile Red in nonpolar solvents.
AuthorsYablon DG, Schilowitz AM
JournalAppl Spectrosc
PubMed ID15282051
The absorbance and fluorescence spectra of Nile Red (NR) were examined in a series of nonpolar solvents comprising linear alkanes and a range of poly alpha olefins (PAO). These solvents span a 1000-fold range in viscosity and possess very similar dielectric constants and refractive index properties. A high-energy double peak ... More
Simple liquid-chromatographic method for Nile Red quantification in cell culture in spite of photobleaching.
AuthorsLamprecht A, Benoit JP
JournalJ Chromatogr B Analyt Technol Biomed Life Sci
PubMed ID12650763
Nile Red fluorescent marker is widely-used for different purposes, such as staining cell structures and for the visualization and localization of colloidal drug carriers. However, when fluorescence-dependent imaging or quantification is performed, the risk of inexact results is increased due to photobleaching. The proposed, simple quantification method of using an ... More
Wide-field subdiffraction imaging by accumulated binding of diffusing probes.
AuthorsSharonov A, Hochstrasser RM
JournalProc Natl Acad Sci U S A
PubMed ID17142314
A method is introduced for subdiffraction imaging that accumulates points by collisional flux. It is based on targeting the surface of objects by fluorescent probes diffusing in the solution. Because the flux of probes at the object is essentially constant over long time periods, the examination of an almost unlimited ... More
An efficient and scalable extraction and quantification method for algal derived biofuel.
Microalgae are capable of synthesizing a multitude of compounds including biofuel precursors and other high value products such as omega-3-fatty acids. However, accurate analysis of the specific compounds produced by microalgae is important since slight variations in saturation and carbon chain length can affect the quality, and thus the value, ... More
Toward systems metabolic engineering in cyanobacteria: opportunities and bottlenecks.
AuthorsNogales J, Gudmundsson S, Thiele I,
JournalBioengineered
PubMed ID23138691
We recently assessed the metabolism of Synechocystis sp PCC6803 through a constraints-based reconstruction and analysis approach and identified its main metabolic properties. These include reduced metabolic robustness, in contrast to a high photosynthetic robustness driving the optimal autotrophic metabolism. Here, we address how these metabolic features affect biotechnological capabilities of ... More
Enhancement of neutral lipid productivity in the microalga Isochrysis affinis Galbana (T-Iso) by a mutation-selection procedure.
AuthorsBougaran G, Rouxel C, Dubois N, Kaas R, Grouas S, Lukomska E, Le Coz JR, Cadoret JP,
JournalBiotechnol Bioeng
PubMed ID22614222
Microalgae offer a high potential for energetic lipid storage as well as high growth rates. They are therefore considered promising candidates for biofuel production, with the selection of high lipid-producing strains a major objective in projects on the development of this technology. We developed a mutation-selection method aimed at increasing ... More
Nile red detection of bacterial hydrocarbons and ketones in a high-throughput format.
A method for use in high-throughput screening of bacteria for the production of long-chain hydrocarbons and ketones by monitoring fluorescent light emission in the presence of Nile red is described. Nile red has previously been used to screen for polyhydroxybutyrate (PHB) and fatty acid esters, but this is the first ... More
The Interplay of Proton, Electron, and Metabolite Supply for Photosynthetic H2 Production in Chlamydomonas reinhardtii.
AuthorsDoebbe A, Keck M, La Russa M, Mussgnug JH, Hankamer B, Tekçe E, Niehaus K, Kruse O,
JournalJ Biol Chem
PubMed ID20581114
To obtain a detailed picture of sulfur deprivation-induced H(2) production in microalgae, metabolome analyses were performed during key time points of the anaerobic H(2) production process of Chlamydomonas reinhardtii. Analyses were performed using gas chromatography coupled to mass spectrometry (GC/MS), two-dimensional gas chromatography combined with time-of-flight mass spectrometry (GCxGC-TOFMS), lipid ... More
Biofuel production in Escherichia coli: the role of metabolic engineering and synthetic biology.
AuthorsClomburg JM, Gonzalez R,
JournalAppl Microbiol Biotechnol
PubMed ID20143230
The microbial production of biofuels is a promising avenue for the development of viable processes for the generation of fuels from sustainable resources. In order to become cost and energy effective, these processes must utilize organisms that can be optimized to efficiently produce candidate fuels from a variety of feedstocks. ... More
A comparison of lipid storage in Phaeodactylum tricornutum and Tetraselmis suecica using laser scanning confocal microscopy.
AuthorsWong DM, Franz AK,
Journal
PubMed ID23933493
Microalgae contain lipid bodies (LBs) composed of triacylglycerols, which can be converted to biodiesel. Here we demonstrate a method to study the accumulation patterns of LBs in different microalgae strains and culture conditions utilizing laser scanning confocal microscopy (LSCM) with BODIPY 505/515 (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene) staining, in parallel with Nile Red (9-diethylamino-5H-benzo-a-phenoxazine-5-one) ... More
CO2-limitation-inducible Green Recovery of fatty acids from cyanobacterial biomass.
AuthorsLiu X, Fallon S, Sheng J, Curtiss R,
JournalProc Natl Acad Sci U S A
PubMed ID21482802
Using genetically modified cyanobacterial strains, we engineered a Green Recovery strategy to convert membrane lipids into fatty acids for economical and environmentally sustainable biofuel production. The Green Recovery strategy utilizes lipolytic enzymes under the control of promoters induced by CO(2) limitation. Data indicate that strains of the cyanobacterium Synechocystis sp. ... More
Fatty acid production in genetically modified cyanobacteria.
AuthorsLiu X, Sheng J, Curtiss R,
JournalProc Natl Acad Sci U S A
PubMed ID21482809
To avoid costly biomass recovery in photosynthetic microbial biofuel production, we genetically modified cyanobacteria to produce and secrete fatty acids. Starting with introducing an acyl-acyl carrier protein thioesterase gene, we made six successive generations of genetic modifications of cyanobacterium Synechocystis sp. PCC6803 wild type (SD100). The fatty acid secretion yield ... More
The fidgety yeast: focus on high-resolution live yeast cell microscopy.
AuthorsWolinski H, Natter K, Kohlwein SD,
JournalMethods Mol Biol
PubMed ID19521820
Despite its small size of 5-8 mum - only one order of magnitude above the wavelength of visible light - yeast has developed into an attractive system for light microscopic analysis. First, the ease of genetic manipulation and integrative transformation have opened numerous experimental strategies for genome-wide tagging approaches, e.g., ... More
Fluorescent phosphocholine--a specific marker for the endoplasmic reticulum and for lipid droplets in Chara internodal cells.
AuthorsFoissner I,
JournalProtoplasma
PubMed ID19795186
The staining pattern of 1,2-bis(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-undecanoyl)-sn-glycero-3-phosphocholine (Bodipy PC) was investigated in internodal cells of the green alga Chara corallina. Ten minutes after dye addition, Bodipy-PC-derived fluorescence appeared in lipid droplets and after 1 h in the cortical endoplasmic reticulum (ER) and in the inner ER tubes. Staining of the ER required ... More
The use of differential scanning fluorimetry to detect ligand interactions that promote protein stability.
AuthorsNiesen FH, Berglund H, Vedadi M,
JournalNat Protoc
PubMed ID17853878
Differential scanning fluorimetry (DSF) is a rapid and inexpensive screening method to identify low-molecular-weight ligands that bind and stabilize purified proteins. The temperature at which a protein unfolds is measured by an increase in the fluorescence of a dye with affinity for hydrophobic parts of the protein, which are exposed ... More
Altered lipid droplet dynamics in hepatocytes lacking triacylglycerol hydrolase expression.
AuthorsWang H, Wei E, Quiroga AD, Sun X, Touret N, Lehner R,
JournalMol Biol Cell
PubMed ID20410140
Lipid droplets (LDs) form from the endoplasmic reticulum (ER) and grow in size by obtaining triacylglycerols (TG). Triacylglycerol hydrolase (TGH), a lipase residing in the ER, is involved in the mobilization of TG stored in LDs for the secretion of very-low-density lipoproteins. In this study, we investigated TGH-mediated changes in ... More
Modulation of membrane fluidity and lipidic metabolism in transformed rat fibroblasts induced by the sesquiterpenic hormone farnesylacetone.
AuthorsRodes JF, Berreur-Bonnenfant J, Trémolières A, Brown SC
JournalCytometry
PubMed ID7736867
Farnesylacetone is a natural terpene extracted from androgenic glands of the crustacean Carcinus maenas and is capable of inhibiting proliferation, notably in transformed mammalian cells. Flow cytometry with three lipophilic probes, diphenylhexatriene, trimethylammonium-diphenylhexatriene, and Nile red, has revealed modifications of the lipidic metabolism in transformed FR3T3-mTT4 rat fibroblasts treated by ... More
Protein gel staining methods: an introduction and overview.
AuthorsSteinberg TH,
JournalMethods Enzymol
PubMed ID19892191
Laboratory scientists who encounter protein biochemistry in many of its myriad forms must often ask: is my protein pure? The most frequent response: run a denaturing SDS polyacrylamide gel. Running this gel raises another series of considerations regarding detection, quantitation, and characterization and so the next questions invariably center on ... More