Air-stable G protein-coupled receptor microarrays and ligand binding characteristics.
AuthorsFang Y, Peng J, Ferrie AM, Burkhalter RS
JournalAnal Chem
PubMed ID16383322
'This paper described novel strategies to achieve air-stable G protein-coupled receptor (GPCR) microarrays and the uses of the microarrays for ligand profiling. Specifically, GPCR cell membrane fragments were suspended in a buffered solution containing bovine serum albumin (BSA) and disaccharide sucrose or trehalose and used for fabricating GPCR microarrays. During ... More
Agonist-antagonist properties of fluorescent opioid probes in the guinea-pig myenteric plexus-longitudinal muscle preparation.
AuthorsKoman A, Einarsson M, Terenius L
JournalNaunyn Schmiedebergs Arch Pharmacol
PubMed ID2419770
'The opioid activity of a series of fluorescent derivatives of oxymorphone, naloxone and naltrexone, were characterized on the electrically stimulated guinea-pig myenteric plexus-longitudinal muscle preparation. In all compounds the fluorescent moiety, fluorescein or tetramethylrhodamine B, is attached to the C-6 carbon of the morphinan. Mu-receptor affinity was well retained and ... More
Fluorescent probes for opioid receptors.
AuthorsKolb VM, Koman A, Terenius L
JournalLife Sci
PubMed ID6319896
Naloxone, naltrexone and oxymorphone were labeled in position 6 with fluorescein by coupling their hydrazone analogs with fluorescein isothiocyanate. Naloxone was also coupled in a similar way with rhodamine-B. The compounds thus obtained were: "6-FN", [1-(N)-fluoresceinyl naloxone thiosemicarbazone]; "6-FNX", [1-(N)-fluoresceinyl naltrexone thiosemicarbazone]; "6-FO", [1-(N)-fluoresceinyl oxymorphone thiosemicarbazone]; "6-RN", [1-(N)-tetramethylrhodaminyl-B naloxone thiosemicarbazone]. ... More
Opioid receptors on guinea-pig intestinal crypt epithelial cells.
AuthorsLang ME, Davison JS, Bates SL, Meddings JB
JournalJ Physiol
PubMed ID8951719
1. Opioid peptides promote net intestinal absorption via two mechanisms: stimulation of Na+ and Cl- absorption and inhibition of Cl- secretion. Although these transport changes are predominantly mediated by submucosal neurones, it is currently unclear whether opioid peptides can regulate enterocyte function directly. We therefore tested the hypothesis that enterocytes ... More