NeuroTrace™ 435/455 蓝色荧光尼氏染色剂

引用和文献 (28)

Invitrogen™

NeuroTrace™ 435/455 蓝色荧光尼氏染色剂

尼氏染色是一种标准组织学方法，用于显示脑和脊髓中的神经元。尼氏物质由神经元核周体和树突中与粗面内质网相关的核糖体 RNA 组成，在损伤或再生神经元的细胞体内再分布，为神经元的生理状态提供标志物。我们的 NeuroTrace 435/455 蓝色荧光尼氏染色剂对神经元的尼氏物质特征具有选择性，并且与甲苯胺蓝或甲酚紫等传统组织学染料相比，其灵敏度更高。了解更多信息

货号	数量
N21479	1 mL

货号 N21479

价格（CNY)

2,832.00

Ends: 31-Dec-2026

3,932.00

共减 1,100.00 (28%)

添加至购物车

价格（CNY)

2,832.00

Ends: 31-Dec-2026

3,932.00

共减 1,100.00 (28%)

添加至购物车

尼氏染色是一种标准组织学方法，用于显示脑和脊髓中的神经元。尼氏物质由神经元核周体和树突中与粗面内质网相关的核糖体 RNA 组成，在损伤或再生神经元的细胞体内再分布，为神经元的生理状态提供标志物。我们的 NeuroTrace 435/455 蓝色荧光尼氏染色剂对神经元的尼氏物质特征具有选择性，并且与甲苯胺蓝或甲酚紫等传统组织学染料相比，其灵敏度更高。

仅供科研使用。不可用于诊断程序。

检测方法荧光

适用于（设备）荧光显微镜

产品类型尼氏染色

数量1 mL

运输条件室温

子细胞定位尼氏体

激发/发射435/455 nm

产品线NEUROTRACE

Unit SizeEach

内容与储存

储存在冰箱（-5 至 -30°C）中并避光。

常见问题解答 (FAQ)

我可以使用NeuroTrace Nissl 染色剂染色胶质或其他类型细胞，该如何改善染色效果，使其对神经元的选择性更强？

我们的NeuroTrace Nissl 染色剂可标记Nissl底物——该底物由与粗面内质网相关的核糖体RNA构成，并大量存在于神经元中。这些染料并非完全特异性靶向神经元，但由于神经元蛋白质的合成水平高而对神经元选择性染色。在某些情况下它们可能会对其他类型细胞（如神经胶质细胞）染色，所以你可能需要降低染色浓度以获得更具选择性的神经元标记效果。对于神经元染色，我们建议的稀释范围是20到300倍。

NeuroTrace Nissl染色剂可染色石蜡切片吗？

我们只在小鼠脑冰冻切片上测试过，但是有文献指出NeuroTrace Nissl 染色剂可染色石蜡切片。

NeuroTrace Nissl 染色剂是什么?

这些染料是保密的，但它们是标记Nissl物质的染色剂。Nissl物质由与粗面内质网相关的核糖体RNA构成，并大量存在于神经元细胞中。

你们有哪些神经元示踪产品？

详细信息请查阅此网页（https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-tracing-tracking-and-morphology/neuronal-tracing.html）。

I am labeling brain cryosections with a NeuroTrace Nissl Stain. Is this compatible with antibody labeling?

Yes. We have done this successfully with an anti-GFAP primary and an Alexa Fluor secondary antibody. We would recommend labeling with the primary and secondary antibodies first, then following up with the standard NeuroTrace Nissl Stain protocol.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all NeuroTrace™ 435/455 蓝色荧光尼氏染色剂 FAQs

引用和文献 (28)

引用和文献

Abstract

Transgenic labeling of the corticospinal tract for monitoring axonal responses to spinal cord injury.

Authors:Bareyre FM, Kerschensteiner M, Misgeld T, Sanes JR

Journal:Nat Med

PubMed ID:16286922

'The rodent corticospinal tract (CST) has been used extensively to investigate regeneration and remodeling of central axons after injury. CST axons are currently visualized after injection of tracer dye, which is invasive, incomplete and prone to variation, and often does not show functionally crucial but numerically minor tract components. Here, ... More

Direction-selective retinal ganglion cells arise from molecularly specified multipotential progenitors.

Authors:De la Huerta I, Kim IJ, Voinescu PE, Sanes JR,

Journal:Proc Natl Acad Sci U S A

PubMed ID:23045641

'Single progenitors can give rise to any and all of the main retinal cell types: photoreceptors, interneurons (horizontal, bipolar, and amacrine cells), retinal ganglion cells (RGCs), and glia. Many of these types are divisible into multiple functionally, structurally, and molecularly distinct subtypes (e.g., ~25 for RGCs). It remains unknown when ... More

In vivo imaging reveals a phase-specific role of STAT3 during central and peripheral nervous system axon regeneration.

Authors:Bareyre FM, Garzorz N, Lang C, Misgeld T, Büning H, Kerschensteiner M,

Journal:Proc Natl Acad Sci U S A

PubMed ID:21447717

'In the peripheral nervous system (PNS), damaged axons regenerate successfully, whereas axons in the CNS fail to regrow. In neurons of the dorsal root ganglia (DRG), which extend branches to both the PNS and CNS, only a PNS lesion but not a CNS lesion induces axonal growth. How this differential ... More

A synaptic basis for auditory-vocal integration in the songbird.

Authors:Bauer EE, Coleman MJ, Roberts TF, Roy A, Prather JF, Mooney R,

Journal:J Neurosci

PubMed ID:18256272

'Songbirds learn to sing by memorizing a tutor song that they then vocally mimic using auditory feedback. This developmental sequence suggests that brain areas that encode auditory memories communicate with brain areas for learned vocal control. In the songbird, the secondary auditory telencephalic region caudal mesopallium (CM) contains neurons that ... More

CO2-sensitive preinspiratory neurons of the parafacial respiratory group express Phox2b in the neonatal rat.

Authors:Onimaru H, Ikeda K, Kawakami K,

Journal:J Neurosci

PubMed ID:19036978

'Phox2b protein is a specific marker for neurons in the parafacial region of the ventral medulla, which are proposed to play a role in central chemoreception and postnatal survival. Mutations of PHOX2B cause congenital central hypoventilation syndrome. However, there have been no reports concerning electrophysiological characteristics of these Phox2b-expressing neurons ... More

View all NeuroTrace™ 435/455 蓝色荧光尼氏染色剂 citations