NeuroTrace™ 530/615 Red Fluorescent Nissl Stain - Solution in DMSO - FAQs

我们的NeuroTrace Nissl 染色剂可标记Nissl底物——该底物由与粗面内质网相关的核糖体RNA构成，并大量存在于神经元中。这些染料并非完全特异性靶向神经元，但由于神经元蛋白质的合成水平高而对神经元选择性染色。在某些情况下它们可能会对其他类型细胞（如神经胶质细胞）染色，所以你可能需要降低染色浓度以获得更具选择性的神经元标记效果。对于神经元染色，我们建议的稀释范围是20到300倍。

我们只在小鼠脑冰冻切片上测试过，但是有文献指出NeuroTrace Nissl 染色剂可染色石蜡切片。

这些染料是保密的，但它们是标记Nissl物质的染色剂。Nissl物质由与粗面内质网相关的核糖体RNA构成，并大量存在于神经元细胞中。 

详细信息请查阅此网页（https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-tracing-tracking-and-morphology/neuronal-tracing.html）。

Yes. We have done this successfully with an anti-GFAP primary and an Alexa Fluor secondary antibody. We would recommend labeling with the primary and secondary antibodies first, then following up with the standard NeuroTrace Nissl Stain protocol.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Our NeuroTrace Nissl stains label the Nissl substance which is composed of ribosomal RNA associated with the rough endoplasmic reticulum and is present in high amounts in neuronal cells. These dyes are not completely specific for neurons, but will selectively stain neurons based on their high level of protein synthesis. In some cases they may show staining of other cell types such as glia, so you may need to decrease the staining concentration to obtain more selective neuronal labeling. We suggest dilutions in the range of 20- to 300-fold for neuronal staining.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

We have only tested them on mouse brain cryosections, however, there is at least one citation describing their use on paraffin tissue sections (Michelle L. Schlief, Ann Marie Craig, and Jonathan D. Gitlin. NMDA Receptor Activation Mediates Copper Homeostasis in Hippocampal Neurons. The Journal of Neuroscience, January 5, 2005, 25(1):239 - 246).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

The dyes are proprietary, however they are stains that label the Nissl substance, which is composed of ribosomal RNA associated with the rough endoplasmic reticulum and is present in high amounts in neuronal cells.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Please check out this web page (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-tracing-tracking-and-morphology/neuronal-tracing.html) for details.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.