NeuroTrace™ 多色组织标记试剂盒 - DiO、DiI、DiD 糊剂,各 500 mg
NeuroTrace™ 多色组织标记试剂盒 - DiO、DiI、DiD 糊剂,各 500 mg
Invitrogen™

NeuroTrace™ 多色组织标记试剂盒 - DiO、DiI、DiD 糊剂,各 500 mg

NeuroTrace™ 多色组织标记试剂盒含有 DiI、DiO 或 DiD 各 500 mg,混合到惰性的抗水凝胶中。这些糊剂为即用型,可使用针尖将其直接应用于活细胞或固定组织标本了解更多信息
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货号数量
N228841 kit
货号 N22884
价格(CNY)
8,911.00
Each
添加至购物车
数量:
1 kit
价格(CNY)
8,911.00
Each
添加至购物车
NeuroTrace™ 多色组织标记试剂盒含有 DiI、DiO 或 DiD 各 500 mg,混合到惰性的抗水凝胶中。这些糊剂为即用型,可使用针尖将其直接应用于活细胞或固定组织标本。这样的使用方式提高了对束状神经元的渗透,使得轴突的上、下表面均得到标记。
仅供科研使用。不可用于诊断程序。
规格
颜色绿色,黄色,红色
检测方法荧光
适用于(设备)荧光显微镜
产品类型组织标记试剂盒
数量1 kit
运输条件室温
子细胞定位细胞膜&脂质
产品线NEUROTRACE™
Unit SizeEach
内容与储存
室温避光储存。

常见问题解答 (FAQ)

亲脂性示踪剂跨膜转运需要多久?FAST亲脂性染料能快多少?

转运过程相当缓慢:在活体组织中大约6 mm/天,在固定组织中更慢。亲脂性羰花青示踪剂从开始给样处扩散到神经元末端,可能需要数天甚至数周的时间。FAST DIO和DiI类似物(具有不饱和烷基尾)可以提高50%左右的转运速度。

我应该使用哪种亲脂性示踪剂(DiO、DiI、DiD等)?

请选择与您现有的激发光源和发射滤光片组/通道兼容的染料。固体、糊和晶体形式的染料可直接施加到组织中的神经元。如需标记培养的细胞或进行显微注射,可采用溶液或固态形式的亲脂性染料。

你们有哪些神经元示踪产品?

详细信息请查阅此网页(https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-tracing-tracking-and-morphology/neuronal-tracing.html)。

I am doing retrograde neuronal labeling using the NeuroTrace DiI Paste. I apply a small glob onto the neuron of interest, but it is not adhering very well. What can I do?

This paste, which has DiI C18 uniformly dispersed within a proprietary hydrophobic carrier, is commonly used to dab onto larger areas than smaller areas commonly used with crystals. Any moisture on the area the paste is applied may be the cause for lack of adherence. Apply the paste using a small flat-head spatula or toothpick and keep it in place a bit longer to ensure contact. The area may also be covered with parafilm to ensure contact as well.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How long does it take for lipophlic tracers to transport along the membrane? How much faster are the FAST lipophilic dyes?

The transport is fairly slow, around 6 mm/day in live tissue and slower in fixed tissue, so diffusion of lipophilic carbocyanine tracers from the point of their application to the terminus of a neuron can take several days to weeks The FAST DiO and DiI analogs (which have unsaturated alkyl tails) can improve transport rate by around 50%.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.