Isolation of DAP3, a novel mediator of interferon-gamma-induced cell death.
AuthorsKissil JL, Deiss LP, Bayewitch M, Raveh T, Khaspekov G, Kimchi A
JournalJ Biol Chem
PubMed ID7499268
'Interaction of certain cytokines with their corresponding cell-surface receptors induces programmed cell death. Interferon-gamma induces in HeLa cells a type of cell death with features characteristic of programmed cell death. Here, we report the isolation of a novel gene, DAP3 (death-associated protein-3), involved in mediating interferon-gamma-induced cell death. The rescue ... More
Rapid movements of vimentin on microtubule tracks: kinesin-dependent assembly of intermediate filament networks.
AuthorsPrahlad V, Yoon M, Moir RD, Vale RD, Goldman RD
JournalJ Cell Biol
PubMed ID9763428
'The assembly and maintenance of an extended intermediate filament (IF) network in fibroblasts requires microtubule (MT) integrity. Using a green fluorescent protein-vimentin construct, and spreading BHK-21 cells as a model system to study IF-MT interactions, we have discovered a novel mechanism involved in the assembly of the vimentin IF cytoskeleton. ... More
Some fluorescent counterstains for neuroanatomical studies.
AuthorsSchmued LC, Swanson LW, Sawchenko PE
JournalJ Histochem Cytochem
PubMed ID6174560
'Methods for counterstaining neural tissue that contains fluorescent markers have been developed. Acridine orange is useful for localizing cells that are retrogradely labelled with the fluorescent tracers true blue, bisbenzimide, and nuclear yellow because at low concentrations it yields a green Nissl stain when excited with blue, but not with ... More
Quantification of adherent and nonadherent cells cultured in 96-well plates using the supravital stain neutral red.
AuthorsLöwik CW, Alblas MJ, van de Ruit M, Papapoulos SE, van der Pluijm G
JournalAnal Biochem
PubMed ID7694524
'In this study we present a rapid, simple, sensitive, inexpensive, and environment-friendly assay for determination of the number of adherent or nonadherent cells cultured in 96-well plates using the supravital stain neutral red. We describe a validation of the method and demonstrate its application to study the effects of hormones ... More
Solvent polarity and pH effects on the spectroscopic properties of neutral red: application to lysosomal microenvironment probing in living cells.
AuthorsSousa C, Sá e Melo T, Gèze M, Gaullier JM, Mazière JC, Santus R
JournalPhotochem Photobiol
PubMed ID8628751
'Neutral red is a lysosomal probe and a biological pH indicator. In aqueous solutions, the protonated (NRH) and neutral (NR) forms of monomeric neutral red exhibit distinct absorption maxima (535 and 450 nm, respectively) but have the same fluorescence with a maximum at 637 nm and a quantum yield of ... More
Neural tube closure in Xenopus laevis involves medial migration, directed protrusive activity, cell intercalation and convergent extension.
AuthorsDavidson LA, Keller RE
JournalDevelopment
PubMed ID10498689
'We have characterized the cell movements and prospective cell identities as neural folds fuse during neural tube formation in Xenopus laevis. A newly developed whole-mount, two-color fluorescent RNA in situ hybridization method, visualized with confocal microscopy, shows that the dorsal neural tube gene xpax3 and the neural-crest-specific gene xslug are ... More
Endocytosis of gastrin in cancer cells expressing gastrin/CCK-B receptor.
AuthorsTarasova NI, Wank SA, Hudson EA, Romanov VI, Czerwinski G, Resau JH, Michejda CJ
JournalCell Tissue Res
PubMed ID8995203
'Endocytosis of gastrin was studied in a number of gastrin-receptor-expressing cell lines by confocal laser scanning microscopy (CLSM) with the aid of a biologically active fluorescent derivative, rhodamine green heptagastrin. Rapid clustering (within 4-7 min) and internalization of fluorescent ligand upon binding at room temperature and 37 degrees C were ... More
Comparison of three in vitro assay systems used for assessing cytotoxic effect of heavy metals on cultured human keratinocytes.
AuthorsYamamoto O, Hamada T, Tokui N, Sasaguri Y
JournalJ UOEH
PubMed ID11279839
'The cell viability assay using cultured cells is of great advantage to elucidate the biological effect of potentially toxic substances. Recently, a novel assay system, Tetracolor One cell proliferation assay (Seikagaku Co., Tokyo, Japan), has been developed. In this report, we compare the results of the Tetracolor One assay regarding ... More
Quantitative in vitro assessment of phototoxicity by a fibroblast-neutral red assay.
AuthorsLasarow RM, Isseroff RR, Gomez EC
JournalJ Invest Dermatol
PubMed ID1569321
'We have adapted the neutral red uptake assay for quantitative assessment of injury to fibroblast cultures by potential phototoxins. Tetracycline derivatives, quinolone derivatives, and chlorpromazine were used as model compounds for development of the assay. Human fibroblasts were incubated with potential phototoxins, the cell cultures irradiated with UV, and the ... More
Structure-activity relationships for diorganotins, chlorinated benzenes, and chlorinated anilines established with bluegill sunfish BF-2 cells.
AuthorsBabich H, Borenfreund E
JournalFundam Appl Toxicol
PubMed ID3356316
'The bluegill sunfish (Lepomis macrochirus) BF-2 cell line, propagated at 34 degrees C, served as target for evaluation of the acute toxicities of various classes of aquatic pollutants, using the neutral red cytotoxicity assay. For a series of chlorinated benzenes and anilines, the sequence of cytotoxicity was dependent on the ... More
Thalictrum minus cell cultures and ABC-like transporter.
AuthorsTerasaka K, Sakai K, Sato F, Yamamoto H, Yazaki K
JournalPhytochemistry
PubMed ID12620361
'Cultured Thalictrum minus cells produce a benzylisoquinoline alkaloid, berberine, in the presence of benzyladenine, and excrete it into the culture medium. T. minus cells excluded berberine, even if berberine was exogenously added to the medium, without benzyladenine treatment. Similarly, T. minus cells excluded a heterocyclic dye (neutral red) and calcein ... More
In vitro cytotoxicity tests on cultured human skin fibroblasts to predict skin irritation potential of surfactants.
AuthorsLee JK, Kim DB, Kim JI, Kim PY
JournalToxicol In Vitro
PubMed ID10906441
'Cultured human skin cells are a potentially useful model for skin irritancy testing. We have investigated the use of human skin fibroblasts for in vitro screening for skin toxicity. To assess the cytotoxic effects of surfactants, cell viability was measured by the NRU (neutral red uptake) assay and AB (Alamar ... More
Glucose alters the susceptibility of mesangial cells to contrast media.
AuthorsWasaki M, Sugimoto J, Shirota K
JournalInvest Radiol
PubMed ID11496090
'RATIONALE AND OBJECTIVES: Diabetic patients frequently suffer contrast media-induced nephropathy. Hyperglycemia in diabetes mellitus causes gradual deterioration of glomerular mesangial cells (MCs) in the kidney. In this study, the authors investigated the response of rat MCs cultured in high-glucose medium to diatrizoate and iohexol, high- and low-osmolar contrast media, respectively. ... More
Comparison of the neutral red and methylene blue assays to study cell growth in culture.
AuthorsElliott WM, Auersperg N
JournalBiotech Histochem
PubMed ID7680581
'The neutral red and methylene blue in vitro cytotoxicity assays were compared under a variety of conditions using normal human ovarian epithelial cells to determine whether either assay is superior for studying cell growth. The results were standardized against a DNA spectrofluorometric assay. Although the assays were equivalent in reflecting ... More
Comparison of in vitro cell cytotoxic assays for tumor necrosis factor.
AuthorsFlick DA, Gifford GE
JournalJ Immunol Methods
PubMed ID6707477
'Four published in vitro assays which measure cell cytotoxicity were compared utilizing murine tumor necrosis factor. These included determination of residual cell number by crystal violet staining in the presence and absence of actinomycin D, lack of viability as determined by neutral red uptake, and [3H]thymidine release in cytotoxin treated ... More
Distribution of intracellular pH in the rat brain cortex after global ischemia as measured by color film histophotometry of neutral red.
AuthorsGriffith JK, Cordisco BR, Lin CW, LaManna JC
JournalBrain Res
PubMed ID1576526
'Tissue acidosis is an important determinant of cell viability following cerebral ischemia. Because of the heterogeneity of tissue response to metabolic stress, a method for measuring intracellular pH (pHi) that preserves spatial information would be desirable. Histophotometry of the pH indicator dye Neutral red offers such a possibility. The purpose ... More
Determination of intracellular pH in the in vitro hippocampal slice preparation by transillumination spectrophotometry of neutral red.
AuthorsSick TJ, Whittingham TS, LaManna JC
JournalJ Neurosci Methods
PubMed ID2918752
'A method is presented for measuring intracellular pH (pHi) spectrophotometrically in hippocampal slices using the pH dye indicator, Neutral red (NR). Measurements of pHi by NR were compared directly with the creatine kinase (CK) equilibrium method. Slices were bathed in artificial cerebrospinal fluid buffered with bicarbonate/CO2. Intracellular pH in hippocampal ... More
Multilayered keratinocyte culture used for in vitro toxicology.
AuthorsHoh A, Maier K, Dreher RM
JournalMol Toxicol
PubMed ID2476674
'Human keratinocytes are the most appropriate target cells for evaluating mechanisms of skin cytotoxicity and pharmacology of chemical agents. After having formed a confluent stratified epithelium with proliferating basal cells and differentiated cell layers, human keratinocytes were harvested after enzymatic detachment as stable, three-dimensional cell aggregates or used as adherent ... More
In vitro staining of islets of Langerhans for fluorescence-activated cell sorting.
'A previously described technique from the author''s laboratories for purification of pancreatic islets by fluorescence-activated cell sorting used the dye neutral red (NR) to obtain specific fluorescence of islets sufficient to give a sorting signal. A major drawback with this technique was the need to inject the dye intravascularly before ... More
Toward using in vitro toxicology in the drug approval process.
AuthorsRatner M
JournalBiotechnology (N Y)
PubMed ID1366957
A view of acidic intracellular compartments.
AuthorsAnderson RG, Orci L
JournalJ Cell Biol
PubMed ID3279044
The use of the dye neutral red as a specific, non-toxic, intra-vital stain of islets of Langerhans.
AuthorsGray DW, Millard PR, McShane P, Morris PJ
JournalBr J Exp Pathol
PubMed ID6196045
The development of improved methods of islet isolation from the pancreas is hampered by difficulty in identification of islets. A method described by Bensley in 1911 uses neutral red to stain the islets selectively. In the present study the method has been simplified and selective staining of islets in the ... More
Experiments on the use of DAMP to study retina and cultured neurons.
AuthorsAugenbraun E, Sulzer D, Rayport S, Setlik W, Holtzman E
JournalJ Histochem Cytochem
PubMed ID1701464
Immunocytochemical localization of DAMP, a reagent used to detect low pH intracellular compartments, was studied in cultured neurons from rat hippocampus and in frog retinas. We find that DAMP is more sharply localized and that the immunocytochemical reaction is stronger when horseradish peroxidase or other proteins are included in the ... More
Distinctive uptake of neutral red by mitotic cancer cells.
AuthorsSit KH, Bay BH, Wong KP
JournalBiotech Histochem
PubMed ID1504181
Neutral red stains both normal and cancer mitotic cells, but uptake by living mitotic cancer cells is distinctly higher than in normal cells. This new approach to cancer cell identification is demonstrated in 4 established tumorigenic cancer cell lines: human skin epidermoid carcinoma A431, mouse Cloudman malignant melanoma, human oral ... More
Short-term quantitative in vitro cytotoxicity assay involving an S-9 activating system.
AuthorsBorenfreund E, Puerner JA
JournalCancer Lett
PubMed ID3828978
Mouse 3T3 fibroblasts grown in 96-well microtiter plates are used for a test which incorporates an S-9 mixed function oxidase system into a neutral red viability assay for the assessment of the acute cytotoxicity of xenobiotics in vitro. This sensitive, quantitative, semi-automated assay was suitable for the rapid screening of ... More
Application of a combination of neutral red and amido black staining for rapid, reliable cytotoxicity testing of biomaterials.
AuthorsCiapetti G, Granchi D, Verri E, Savarino L, Cavedagna D, Pizzoferrato A
JournalBiomaterials
PubMed ID8805972
Cell viability and growth for cytotoxicity evaluation of materials for prosthetic devices has been tested using various methods. The aim of this study was to extend the choice of reliable methods to quantify cytotoxicity of materials in vitro. By measuring both viability and growth of cells exposed to biomaterials in ... More
Neutral red uptake assay for the estimation of cell viability/cytotoxicity.
AuthorsRepetto G, del Peso A, Zurita JL,
JournalNat Protoc
PubMed ID18600217
The neutral red uptake assay provides a quantitative estimation of the number of viable cells in a culture. It is one of the most used cytotoxicity tests with many biomedical and environmental applications. It is based on the ability of viable cells to incorporate and bind the supravital dye neutral ... More
A vacuolar-type H+-pyrophosphatase governs maintenance of functional acidocalcisomes and growth of the insect and mammalian forms of Trypanosoma brucei.
AuthorsLemercier G, Dutoya S, Luo S, Ruiz FA, Rodrigues CO, Baltz T, Docampo R, Bakalara N,
JournalJ Biol Chem
PubMed ID12121996
Vacuolar proton pyrophosphatases (V-H(+)-PPases) are electrogenic proton pumps found in many organisms of considerable industrial, environmental, and clinical importance. V-H(+)-PPases of several parasites were shown to be associated with acidic vacuoles named acidocalcisomes, which contain polyphosphate and calcium. In this work we functionally characterized a Trypanosoma brucei V-H(+)-PPase gene by ... More
Uptake kinetics and intracellular localization of hypocrellin photosensitizers for photodynamic therapy: a confocal microscopy study.
AuthorsMiller GG, Brown K, Moore RB, Diwu ZJ, Liu J, Huang L, Lown JW, Begg DA, Chlumecky V, Tulip J
JournalPhotochem Photobiol
PubMed ID7568409
Hypocrellins are naturally occurring compounds with photosensitizing properties in biological systems. We have prepared synthetic derivatives of hypocrellin B, which have promise as photosensitizers in the clinical application of photodynamic therapy. The intracellular localization and uptake kinetics of hypocrellin B and several selected hypocrellin congeners were determined semiquantitatively by fluorescence ... More
The use of neutral red as an intracellular pH indicator in rat brain cortex in vivo.
AuthorsLaManna JC, McCracken KA
JournalAnal Biochem
PubMed ID6517307
Intracellular pH in the intact, normally perfused rat brain cortex was determined by rapid scanning reflectance spectrophotometry of Neutral Red. Neutral Red, a pH indicator dye, was administered intraperitoneally to rats. Reflectance spectra recorded from the exposed dural surface of 11 anesthetized rats were used to calculate an intracellular pH ... More
Ability of 16 priority PAHs to be directly cytotoxic to a cell line from the rainbow trout gill.
Sixteen polycyclic aromatic hydrocarbons (PAHs) were screened for their ability to be directly cytotoxic to a cell line from the rainbow trout gill, RTgill-W1. Exposure times of 2 h or less were sufficient for direct cytotoxicity to be detected, which appeared to be caused by a common mechanism, the general ... More
A sensitive new bioassay for tumor necrosis factor.
AuthorsShahan TA, Siegel PD, Sorenson WG, Kuschner WG, Lewis DM
JournalJ Immunol Methods
PubMed ID7930647
Tumor necrosis factor is an important cytokine involved in inflammation and assay of this cytokine in biological fluids may be important in the understanding of several disease processes. This report describes an improved TNF bioassay employing a newly isolated subclone of the cell line NCTC-clone 929 as well as a ... More
Fluorescent cationic probes for nuclei of living cells: why are they selective? A quantitative structure-activity relations analysis.
AuthorsHorobin RW, Stockert JC, Rashid-Doubell F
JournalHistochem Cell Biol
PubMed ID16463179
Selectivity of nuclear probes is controlled by competitive accumulation of the probe by cellular organelles as well as the high affinity for nucleic acids. Physicochemical features of probes which favor nucleic acid binding include cationic character and a planar aromatic system above a minimum size. Features of probes which permit ... More
Localization of D1 dopamine receptors on live cultured striatal neurons by quantitative fluorescence microscopy.
AuthorsHoyt KR, Reynolds IJ
JournalBrain Res
PubMed ID8883850
Single neurons in culture express a heterogeneity of neurotransmitter receptor subtypes. The study of the effects of neurotransmitters on neuronal function is complicated by this heterogeneity. It would therefore be useful to be able to identify live neurons that express the receptors of interest and then use these neurons for ... More
Apigenin and tangeretin enhance gap junctional intercellular communication in rat liver epithelial cells.
AuthorsChaumontet C, Bex V, Gaillard-Sanchez I, Seillan-Heberden C, Suschetet M, Martel P
JournalCarcinogenesis
PubMed ID7955073
Two flavones, apigenin and tangeretin, were studied for their ability to modulate gap junctional intercellular communication (GJIC) in the rat liver epithelial cell line REL. Their cytotoxicity was first determined by cell density and neutral red uptake assays: neither apigenin nor tangeretin are cytotoxic at 10 and 25 microM, the ... More
Partial characterization of cytoplasmic compartments involved in the endocytic process of Tritrichomonas foetus.
AuthorsAffonso AL, de Almeida JC, Benchimol M
JournalEur J Cell Biol
PubMed ID9084987
The endocytic pathway of Tritrichomonas foetus, a parasitic protozoan of cattle, was studied using (a) vital dyes, such as Lucifer yellow, neutral red and acridine orange, (b) cationized ferritin, (c) gold-labeled lactoferrin and lectins: HPA, UEA, PNA and LPA, and (d) DAMP (3-(2,4-dinitroanilino) 3' amino-N-methyldipropylamine). Light and confocal laser microscopy ... More
Histochemical probing of potato periderm with neutral red: a sensitive cytofluorochrome for the hydrophobic domain of suberin.
AuthorsLulai EC, Morgan WC
JournalBiotech Histochem
PubMed ID1504180
A technique is described which uses the lipid fluorochrome neutral red as a cytochemical probe to detect the hydrophobic domain of the ligno-suberin matrix in native and wound periderm of potato tuber. Toluidine blue O is used as a counterstain to quench autofluorescence. The neutral red technique appears to be ... More
Flow cytometric assay of phagocytic activity of human neutrophils and monocytes in whole blood by neutral red uptake.
AuthorsAntal P, Sipka S, Surányi P, Csipo I, Seres T, Maródi L, Szegedi G
JournalAnn Hematol
PubMed ID7599287
In a new, simple, and fast flow-cytometric method for the simultaneous measurement of phagocytic activity of human neutrophils and monocytes in whole blood, the fluorescence capability of the well-known vital stain, neutral red was used. The incubation of 0.5 ml heparinized human blood with the phagocytic stimulus of zymosan dose- ... More
Predicting the behaviour and selectivity of fluorescent probes for lysosomes and related structures by means of structure-activity models.
AuthorsRashid F, Horobin RW, Williams MA
JournalHistochem J
PubMed ID1743993
Cultured rat fibroblasts were exposed to 50 fluorescent probes of varied physicochemical characteristics. Probe concentrations, fluorochrome excitation wavelength and period of illumination, and cell-probe contact time were varied. Structure-activity relationships defining a number of classes of fluorescent probes for lysosomes and related processes and compartments were demonstrated. Numerical specifications are ... More
Change in colony morphology influences the virulence as well as the biochemical properties of the Mycobacterium avium complex.
AuthorsKansal RG, Gomez-Flores R, Mehta RT
JournalMicrob Pathog
PubMed ID9817824
Factors that influence colony morphology are of crucial importance for drug development as well as for understanding the virulence of Mycobacterium avium complex (MAC) strains. The MAC 101 strain used in the present study grows as smooth transparent (SmT) colonies that tend to become opaque and pigmented when incubated for ... More
Induction by xenobiotics of phase I and phase II enzyme activities in the human keratinocyte cell line NCTC 2544.
AuthorsGelardi A, Morini F, Dusatti F, Penco S, Ferro M
JournalToxicol In Vitro
PubMed ID11698172
This study analyses the expression and induction of several drug-metabolising enzyme activities involved in either phase I or phase II biotransformations in NCTC 2544 human keratinocytes. The phase I activities 7-ethoxycoumarin O-deethylase (ECOD), 7-ethoxyresorufin O-deethylase (EROD) and 7-pentoxyresorufin O-depenthylase (PROD) were easily detectable in basal conditions. During incubations lasting up ... More
Acidification and recovery results in nuclear accumulation of supravital dyes during interphase.
AuthorsSit KH, Paramanantham R, Bay BH, Wong KP
JournalBiotech Histochem
PubMed ID8874854
Recent studies using real time imaging demonstrated relative nuclear insulation for ion-size particles. We show here that acidification and recovery converted the insulated interphase nuclei of KB carcinoma and nontumorigenic Chang cells into intense nuclear accumulating states marked by sequestration of the exogenous supravital dyes neutral red, methylene blue, and ... More
Sleep and respiration under acute hypobaric hypoxia.
AuthorsMizuno K, Asano K, Okudaira N
JournalJpn J Physiol
PubMed ID8355417
The purpose of this study was to elucidate the effects of acute hypobaric hypoxia on nocturnal sleep architecture and respiratory responses in a hypobaric simulator. Five healthy young males (19-23 years old) were recruited to sleep for 8 h at sea level and at simulated altitudes of 1,500, 3,000, and ... More
The role of the lysosome in natural killing: inhibition by lysosomotropic vital dyes.
AuthorsShau H, Dawson JR
JournalImmunology
PubMed ID6500627
Recent evidence has indicated the importance of lysosomes in natural killing (NK). The lysosomotropic dye neutral red, at concentrations greater than 2 X 10(-5) M, inhibits NK activity of large granular lymphocytes (LGL). Inhibition is at the level of effector cell activity rather than target cell susceptibility. Neutral red inhibits ... More
Arbuscules of vesicular-arbuscular mycorrhizal fungi inhabit an acidic compartment within plant roots.
AuthorsGuttenberger M
JournalPlanta
PubMed ID10987547
The most widespread type of mycorrhiza is the so-called vesicular-arbuscular mycorrhiza. In this endomycorrhiza, fungal hyphae penetrate plant cell walls in the root cortex. There they form densely branched arbuscules. Fungus and plant plasma membrane are separated by a common interfacial apoplast. The pH of the compartment between the symbionts ... More
Mediating role of metabolic activation in in vitro cytotoxicity assays.
AuthorsBabich H, Martin-Alguacil N, Borenfreund E
JournalMol Toxicol
PubMed ID3151501
Enzymatic activation of polycyclic aromatic hydrocarbons (PAHs) and its effect on cytotoxicity were studied using the neutral red viability assay as the end point. Benzo[a]pyrene was progressively cytotoxic to human hepatoma (HepG2) cells over a 1- to 3-d period, and after induction of monooxygenase activity with a polychlorobiphenyl (PCB) mixture ... More
Length of the linking domain of human pro-tumor necrosis factor determines the cleavage processing.
AuthorsTang P, Hung M-C Klostergaard J
JournalBiochemistry
PubMed ID8679577
Several studies have indicated that only one cleavage site (Ala-1/Val+1) is involved in the release of mature TNF from human pro-TNF, whereas others have suggested that the linking sequence (residues -20 to -1) may be important. We previously demonstrated that a pro-TNF deletion mutant, delta -20- -1, was able to ... More
Neutral red (NR) assay for cell viability and xenobiotic-induced cytotoxicity in primary cultures of human and rat hepatocytes.
AuthorsZhang SZ, Lipsky MM, Trump BF, Hsu IC
JournalCell Biol Toxicol
PubMed ID2113829
Neutral red (NR) in medium was absorbed and concentrated in lysosomes of cultured rat and human hepatocytes. NR uptake increased with the time of incubation and reached a plateau in 2 hr. Uptake was proportional to the concentration of the NR solution and the numbers of viable liver cells. Prolonged ... More
Proton movements and electric potential generation in reconstituted ATPase proteoliposomes from the thermophilic cyanobacterium Synechococcus 6716.
AuthorsVan Walraven HS, Marvin HJ, Koppenaal E, Kraayenhof R
JournalEur J Biochem
PubMed ID6092075
ATP hydrolysis-induced proton translocation and electric potential generation have been studied in ATPase proteoliposomes by means of various optical probes. The proteoliposomes consisted of reconstituted ATPase complex and native lipid mixture isolated from the thermophilic cyanobacterium Synechococcus 6716 [Van Walraven et al. (1983) Eur. J. Biochem. 137, 101-106]. The native ... More
Distribution of furamidine analogues in tumor cells: targeting of the nucleus or mitochondria depending on the amidine substitution.
AuthorsLansiaux A, Tanious F, Mishal Z, Dassonneville L, Kumar A, Stephens CE, Hu Q, Wilson WD, Boykin DW, Bailly C
JournalCancer Res
PubMed ID12499262
Diphenylfuran diamidines represent an important class of DNA minor groove binders of high therapeutic interest as antiparasitic or antitumor agents depending on the compounds structures. To exert their cytotoxic action, the compounds must first get into the cell and reach the nuclear compartment where the main target, DNA, is located. ... More
New in vitro fluorimetric microtitration assays for toxicological screening of drugs.
AuthorsRat P, Korwin-Zmijowska C, Warnet JM, Adolphe M
JournalCell Biol Toxicol
PubMed ID7697493
Flow cytometry has been widely used to quantify fluorescent probes in cell culture. However, FCM is not adapted to toxicological screenings due to the cost, the length and the poor reproducibility of this technique. Moreover, several multicenter studies have preferred microtitration methodologies for drug screening. A new fluorimetric technology has ... More
Neutral red as a probe for confocal laser scanning microscopy studies of plant roots.
AuthorsDubrovsky JG, Guttenberger M, Saralegui A, Napsucialy-Mendivil S, Voigt B, Baluska F, Menzel D
JournalAnn Bot (Lond)
PubMed ID16520341
BACKGROUND AND AIMS: Neutral red (NR), a lipophilic phenazine dye, has been widely used in various biological systems as a vital stain for bright-field microscopy. In its unprotonated form it penetrates the plasma membrane and tonoplast of viable plant cells, then due to protonation it becomes trapped in acidic compartments. ... More
[Antioxidant effects of mast cell inhibitors in a human conjunctival cell line]
AuthorsDebbasch C, Pisella PJ, Rat P, Warnet JM, Baudouin C
JournalJ Fr Ophtalmol
PubMed ID11240482
PURPOSE: The aim of this study was to evaluate in vitro antioxidant effects of two mast cells inhibitors. METHODS: Cytotoxicity tests were done on a continuous human conjunctival cell line using microplate cold light cytofluorimetry. Membrane integrity (neutral red test), DNA condensation (Hoechst 33342 test), and reactive oxygen species (ROS) ... More
Tracking of proton flow during transition from anaerobiosis to steady state. 1. Response of matrix pH indicators.
AuthorsLuvisetto S, Schmehl I, Cola C, Azzone GF
JournalEur J Biochem
PubMed ID1657603
1. The kinetics of acidification and realkalinization of the matrix after addition of nigericin to respiring and non-respiring mitochondria, recorded by intramitochondrial pH indicators such as neutral red and 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein (BCECF), is complementary to that recorded by extramitochondrial pH indicators. The extent of acidification decreases with the logarithm of the ... More
Chromium- and nickel-induced cytotoxicity in normal and transformed human keratinocytes: an investigation of pharmacological approaches to the prevention of Cr(VI)-induced cytotoxicity.
AuthorsLittle MC, Gawkrodger DJ, MacNeil S
JournalBr J Dermatol
PubMed ID8746330
Chromium and nickel compounds cause irritancy but can also induce allergic contact dermatitis. The aims of this study were to characterize the direct cytotoxic effects of Cr(VI), Cr(III) and Ni(II) salts on keratinocytes, and to investigate pharmacological strategies to protect cells against Cr(VI)-induced cytotoxicity. Normal human keratinocytes and the HaCaT ... More
Fibroblast growth factor-2 suppression of tumor necrosis factor alpha-mediated apoptosis requires Ras and the activation of mitogen-activated protein kinase.
AuthorsGardner AM, Johnson GL
JournalJ Biol Chem
PubMed ID8662985
Treatment of L929 cells with tumor necrosis factor alpha (TNFalpha) activates a programmed cell death pathway resulting in apoptosis. We investigated the intracellular signaling pathways activated in L929 cells by TNFalpha. TNFalpha robustly activates Jun kinase (JNK), a member of the mitogen-activated protein kinase (MAPK) family. In addition, p42(MAPK) is ... More
The tight junctions protein Claudin-5 limits endothelial cell motility.
Authors
JournalJ Cell Sci
PubMed ID34005418
The tight junction protein Claudin-5 limits endothelial cell motility.
Authors
JournalJ Cell Sci
PubMed ID33323504
Sea urchin embryos as a model system for studying autophagy induced by cadmium stress.