NBD Phallacidin - FAQs

2 个常见问题解答

我试着用鬼笔环肽结合F-actin标记我的石蜡切片，但是没有看到信号，为什么？

当细胞和组织经由二甲苯或丙酮之类的溶剂处理时（例如组织切片脱石蜡期间），它通过阻止鬼笔环肽结合的方式来影响F- actin。冰冻切片通常不使用有机溶剂洗涤，可以用来代替石蜡切片，也可以使用抗肌动蛋白抗体。

I'm trying to label my paraffin sections for F-actin with a phalloidin conjugate, but I'm not seeing any signal. Why?

When cells and tissues are treated with solvents such as xylene or acetone (for example during deparaffinization of tissue sections), it affects the F-actin in a way that prevents phalloidins from binding. Phalloidin may be used with cryosections, which are not typically washed with organic solvents, or anti-actin antibodies may be used.

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