Expression cloning of a high-affinity melatonin receptor from Xenopus dermal melanophores.
AuthorsEbisawa T, Karne S, Lerner MR, Reppert SM
JournalProc Natl Acad Sci U S A
PubMed ID7517042
Using an expression cloning strategy, a high-affinity melatonin receptor cDNA has been isolated from Xenopus laevis dermal melanophores. Transient expression of the cDNA in COS-7 cells resulted in high-affinity 2-[125I]-iodomelatonin binding (Kd = 6.3 +/- 0.3 x 10(- 11) M). In addition, six ligands exhibited a rank order of inhibition ... More
Expression cloning of a receptor for murine granulocyte colony- stimulating factor.
AuthorsFukunaga R, Ishizaka-Ikeda E, Seto Y, Nagata S
JournalCell
PubMed ID2158861
Two cDNAs encoding the receptor for murine granulocyte colony- stimulating factor (G-CSF) were isolated from a CDM8 expression library of mouse myeloid leukemia NFS-60 cells, and their nucleotide sequences were determined. Murine G-CSF receptor expressed in COS cells could bind G-CSF with an affinity and specificity similar to that of ... More