Fluorescent lipid uptake and transport in adult Schistosoma mansoni.
AuthorsMoffat D, Kusel JR
JournalParasitology
PubMed ID1437279
'Fluorescent lipophilic compounds can be used to label the surface membrane of Schistosoma mansoni by adding the compound in small amounts of organic solvents to aqueous medium in vitro. Under these conditions it is difficult to follow routes of distribution of the label. Here we have absorbed nitrobenzoxadiazolamine methylamino-(NBD)-ceramides to ... More
Gaucher type I (Ashkenazi) disease: a new method for heterozygote detection using a novel fluorescent natural substrate.
AuthorsGrabowski GA, Dinur T, Gatt S, Desnick RJ
JournalClin Chim Acta
PubMed ID6889929
'A new acid beta-glucosidase assay for the detection of heterozygotes for Gaucher Type I disease has been developed using isolated lymphocytes as enzyme source and a novel fluorescent natural substrate, NBD-glucosyl ceramide. The procedure for optimal heterozygote discrimination was established by systematic evaluation of the effect of various solubilization techniques, ... More
Fluorescence assay of glucosylceramide glucosidase using NBD-cerebroside.
AuthorsAbe A, Shayman JA, Radin NS
JournalLipids
PubMed ID1487952
'A sensitive fluorometric assay for glucocerebroside beta-glucosidase [Dinur, T., Grabowski, G.A., Desnick, R.J., and Gatt, S. (1984) Anal. Biochem. 136, 223-234] has been reexamined. It was found that the lipids containing the NBD moiety (12-[N-methyl-N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)] used for standardization of the assay are light-sensitive and that the yield of fluorescent light ... More
Lateral diffusion of lipids in model and natural membranes.
AuthorsTocanne JF, Dupou-Cézanne L, Lopez A
JournalProg Lipid Res
PubMed ID8022844
Prenatal diagnosis of Krabbe disease using a fluorescent derivative of galactosylceramide.
AuthorsZeigler M, Zlotogora J, Regev R, Dagan A, Gatt S, Bach G
JournalClin Chim Acta
PubMed ID6488564
A fluorescent substrate 12-(N-methyl-N(7-nitro-2-oxa-1,3-diazol-4-yl) aminododecanoyl sphingosyl beta-D-galactoside ('NBD galactocerebroside') was synthesized and used for the detection of galactocerebrosidase activity. The enzyme determinations using this substrate were found to be extremely sensitive yielding unambiguous results. This substrate was used for the prenatal diagnosis of a fetus affected with Krabbe disease; the ... More
Large-scale preparation of asymmetrically labeled fluorescent lipid vesicles.
AuthorsDao HN, McIntyre JC, Sleight RG
JournalAnal Biochem
PubMed ID1888035
A method for producing lipid vesicles containing fluorescent phospholipid analogues localized to the inner leaflet of their membrane was developed. Incubation of a 450-fold molar excess of serum albumin with lipid vesicles symmetrically labeled with 1 mol % 1-palmitoyl-2-(N-4-nitrobenzo-2-oxa-1,3-diazolyl)amino-caproyl phosphatidylcholine resulted in the removal of 99% of the fluorescent lipid ... More
Purification and characterization of a neutral ceramidase from mouse liver. A single protein catalyzes the reversible reaction in which ceramide is both hydrolyzed and synthesized.
AuthorsTani M, Okino N, Mitsutake S, Tanigawa T, Izu H, Ito M
JournalJ Biol Chem
PubMed ID10652340
We report here a novel ceramidase that was purified more than 150, 000-fold from the membrane fraction of mouse liver. The enzyme was a monomeric polypeptide having a molecular mass of 94 kDa and was highly glycosylated with N-glycans. The amino acid sequence of a fragment obtained from the purified ... More
Human acid beta-glucosidase: use of inhibitors, alternative substrates and amphiphiles to investigate the properties of the normal and Gaucher disease active sites.
AuthorsOsiecki-Newman K, Fabbro D, Legler G, Desnick RJ, Grabowski GA
JournalBiochim Biophys Acta
PubMed ID2956992
Comparative studies with lipoidal inhibitors and alternative substrates were conducted to investigate the properties of the active site of human acid beta-glucosidase (D-glucosyl-N-acylsphingosine glucohydrolase, EC 3.2.1.45) from normal placenta and spleens of Type 1 Ashkenazi Jewish Gaucher disease (AJGD) patients. With the normal enzyme, the inhibitory potencies of series of ... More