Kinetics of the phosphorylation of Na,K-ATPase by inorganic phosphate detected by a fluorescence method.
AuthorsApell HJ, Roudna M, Corrie JE, Trentham DR
JournalBiochemistry
PubMed ID8718885
'Phosphorylation by Pi of the Na,K-ATPase from rabbit kidney in the absence of Na+ ions but in the presence of Mg2+ ions has been studied. In the absence of K+ ions, unphosphorylated and phosphorylated states induce different fluorescence levels in the membrane-bound styryl dye RH421, and hence transitions between the ... More
NAADP controls cross-talk between distinct Ca2+ stores in the heart.
AuthorsMacgregor A, Yamasaki M, Rakovic S, Sanders L, Parkesh R, Churchill GC, Galione A, Terrar DA
JournalJ Biol Chem
PubMed ID17387177
'In cardiac muscle the sarcoplasmic reticulum (SR) plays a key role in the control of contraction, releasing Ca(2+) in response to Ca(2+) influx across the sarcolemma via voltage-gated Ca(2+) channels. Here we report evidence for an additional distinct Ca(2+) store and for actions of nicotinic acid adenine dinucleotide phosphate (NAADP) ... More
The kinetics of magnesium adenosine triphosphate cleavage in skinned muscle fibres of the rabbit.
AuthorsFerenczi MA, Homsher E, Trentham DR
JournalJ Physiol
PubMed ID6611412
'The time course of magnesium adenosine triphosphate (Mg ATP) cleavage in chemically skinned muscle fibres of the rabbit was measured by a method in which Mg ATP cleavage was initiated by photolytic release of ATP from P3-1-(2-nitro)phenylethyladenosine 5'-triphosphate (caged ATP) and terminated by rapid freezing 50 ms to 8 s ... More
Strain-dependent modulation of phosphate transients in rabbit skeletal muscle fibers.
AuthorsHomsher E, Lacktis J, Regnier M
JournalBiophys J
PubMed ID9083682
'When inorganic phosphate (Pi) is photogenerated from caged Pi during isometric contractions of glycerinated rabbit psoas muscle fibers, the released Pi binds to cross-bridges and reverses the working stroke of cross-bridges. The consequent force decline, the Pi-transient, is exponential and probes the kinetics of the power-stroke and Pi release. During ... More
Rapid photolytic release of adenosine 5'-triphosphate from a protected analogue: utilization by the Na:K pump of human red blood cell ghosts.
AuthorsKaplan JH, Forbush B, Hoffman JF
JournalBiochemistry
PubMed ID148906
Caged cysteine and thiophosphoryl peptides.
AuthorsPan P, Bayley H
JournalFEBS Lett
PubMed ID9094429
Photoreleasable molecules are important in studies of various biological phenomena, especially cell signaling. Here we report a generally applicable approach for 'caging' unprotected cysteine-containing or thiophosphorylated peptides in aqueous solution with 2-nitrobenzyl bromides. Photolysis of the caged peptides was achieved with near UV light with product quantum efficiencies of 0.064-0.62 ... More
Reversal of the cross-bridge force-generating transition by photogeneration of phosphate in rabbit psoas muscle fibres.
AuthorsDantzig JA, Goldman YE, Millar NC, Lacktis J, Homsher E
JournalJ Physiol
PubMed ID1403812
1. Orthophosphate (P(i), 0.1-2.0 mM) was photogenerated within the filament lattice of isometrically contracting glycerinated fibres of rabbit psoas muscle at 10 and 20 degrees C. The P(i) was produced by laser flash photolysis of the photolabile compound 1-(2-nitrophenyl)ethylphosphate (caged P(i)). Caged P(i) caused a depression of tension that was ... More