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Human Neural Stem Cells (H9-Derived) Kit - FAQs

查看更多产品信息 Human Neural Stem Cells (H9-Derived) Kit - FAQs (N7800200)

10 个常见问题解答

H9来源的人NSC能分化为多巴胺能神经元么？

我们不推荐将H9来源的NSCs分化为多巴胺能神经元。这些NSCs能分化为普通神经元，而非特殊种类的神经元。

神经干细胞（NSC）培养中使用的生长因子或细胞因子有哪些？

以下生长因子可用于NSC的扩增：重组EGF（货号PHG0314），重组bFGF（货号PHG0024)，和重组VEGF（货号PHC9394）。此外，包括BDNF在内的多几种神经营养因子例如BDNF（货号10908010），CNTF（货号PHC7015），和GDNF（货号PHC7044）也可适用于相关研究。

我应如何培养人神经干细胞（NSC）？

人NSC可培养于Gibco StemPro NSC SFM（货号A1050901）和Gibco Geltrex 基质/或Gibco CELLstart 底物基质预先包被的培养皿中。另外，如果用户的研究目的是获得神经元，也可在已添加了Gibco B-27添加剂（不含维生素A）的Neurobasal培养基（不含维生素A） + 预先包被的培养皿中培养NSC。

我应如何鉴定神经干细胞（NSC）？

当按照克隆密度进行培养时，通常可基于用神经球的形成能力来鉴定NSC（Nat Methods 2:333 (2005)）。也可通过（1）Sox1，Sox2和Nestin的RT-PCR检测或（2）nestin，Pax6，Sox2和Ki67的免疫组化染色来鉴定NSC。

何为神经干细胞（NSC）？

神经干细胞（NSC）是神经系统中能够自我更新的多专能细胞，拥有分化为神经元，少突胶质细胞和星形胶质细胞的潜能。神经干细胞可由胚胎干细胞诱导分化而来，或从皮层，脑室下区（SVZ）和脑室区等多个不同脑区分离获得，或从骨髓来源的间充质干细胞（MSC）生成 (J Cell Biochem 114:764 (2013))。NSC是研究神经发生和神经递质与受体功能的宝贵工具。来自在各类动物疾病模型中的NSC被广泛应用于包括例如帕金森氏病与亨廷顿氏病舞蹈症在内的等多种不同类型的CNS疾病的研究中（J Cell Biochem 114:764 (2013)）。

Can H9-derived human NSCs be differentiated into dopaminergic neurons?

We do not recommend using H9-derived NSCs to differentiate into dopaminergic neurons. These NSCs can be differentiated into general neurons, but not specific neurons.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What are the growth factors or cytokines used in neural stem cell (NSC) culture?

For NSC expansion, the following growth factors are used: recombinant EGF (Cat. No. PHG0314), recombinant FGF-basic (Cat. No. PHG0024), and recombinant VEGF (Cat. No. PHC9394). In addition, several neurotrophins such as BDNF Cat. No. 10908010), NT-3, CNTF (Cat. No. PHC7015), and GDNF (Cat. No. PHC7044)are also used in the related studies.

Information pertaining to whether a specific product has been tested against the WHO Reference Standard can typically be located on the product page or Certificate of Analysis (COA).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I culture human neural stem cells (NSCs)?

Human NSCs can grow in Gibco StemPro NSC SFM (Cat. No. A1050901) on dishes pre-coated with Gibco Geltrex Matrix or Gibco CELLstart substrate. Alternatively, if the goal is to obtain neurons, NSCs can also be grown on Neurobasal medium supplemented with Gibco B-27 supplements without vitamin A on a pre-coated dish.

How can I characterize neural stem cells (NSCs)?

NSCs are generally characterized by their ability to form neurospheres when plated at cloning density (Nat Methods 2:333 (2005)). NSCs can also be characterized by (1) RT-PCR of Sox1, Sox2, and Nestin or (2) immunohistochemical staining for nestin, Pax6, Sox2, and Ki67.

What are neural stem cells?

Neural stem cells (NSCs) are self-renewing multipotent cells of the nervous system capable of differentiating into neurons, oligodendrocytes, and astrocytes. NSC can be generated by induced differentiation from embryonic stem (ES) cells, or isolated from various regions of the brain including the cortex, the subventricular zone (SVZ), and the ventricular zone, or generated from bone marrow-derived mesenchymal stem cells (MSCs) (J Cell Biochem 114:764 (2013)). NSCs are valuable tools for the study of neurogenesis and neurotransmitter and receptor function. NSCs were used in the investigation of different CNS disorders such as PD and Huntington's disease in various animal models (J Cell Biochem 114:764 (2013)).