Newport Green™ DCF 二乙酸酯,细胞可透过性
Newport Green™ DCF 二乙酸酯,细胞可透过性
Invitrogen™

Newport Green™ DCF 二乙酸酯,细胞可透过性

Newport Green™ DCF 指示剂具有中度锌结合亲和力(对 Zn2+ 的 Kd ∼为 1了解更多信息
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货号数量
N79911 mg
货号 N7991
价格(CNY)
3,896.00
飞享价
Ends: 31-Dec-2025
5,110.00
共减 1,214.00 (24%)
Each
添加至购物车
数量:
1 mg
价格(CNY)
3,896.00
飞享价
Ends: 31-Dec-2025
5,110.00
共减 1,214.00 (24%)
Each
添加至购物车
Newport Green™ DCF 指示剂具有中度锌结合亲和力(对 Zn2+ 的 Kd ∼为 1 µM)但基本对 Ca2+ 不敏感(对 Ca2+ 的 Kd >100 µM),使其成为一种有价值的探针,用于检测通过电压门控通道或谷氨酸门控通道进入神经元的 Zn2+。当与具有双重 Ca2+/Zn2+ 敏感性的染料(如 fura-2 和 mag-fura-2)一起使用时,Newport Green™ DCF 确认可检测到 Zn2+ 水平变化,而不能检测到 Ca2+ 或 Mg2+ 水平变化。表征了 Newport Green™ DCF 对过渡金属(包括 Zn、Mn、Fe、Co、Cu(I)、Cu(II)、Ni 和 Cd)的响应。

了解有关离子指示剂(包括钙、钾、pH 值和膜电位指示剂)的更多信息›

与 Newport Green™ DCF 指示剂相比,Newport Green™ PDX 具有较高的 Zn2+ 解离常数(对 Zn2+ 的 Kd 为 ∼30 µM)和较大的 Zn2+-游离至 Zn2+-饱和荧光强度增幅。

荧光锌指示剂规格:
• 标记(激发/发射波长):Newport Green™ DCF (∼505/535 nm)
• Kd (Zn2+)(缓冲液中):∼1 µM
• 冻干产品可以溶解在 DMSO 中,以供使用
•通常,通过向含细胞的培养基中添加溶解指示剂将产品上样至细胞中


查找锌和其他金属离子的荧光指示剂
我们提供多种荧光指示剂来确定细胞内多价阳离子的浓度、跟踪通过离子通道进行的金属离子转运、对环境样品进行测量等等。有关这些产品的更多信息,请查看 Zn2+ 和其他金属离子的荧光指示剂 — Molecular Probes™ 手册中的 19.7 节

仅供科研使用。不可用于人或动物的治疗或诊断。
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光
染料类型锌指示剂
数量1 mg
运输条件室温
适用于(应用)细胞活力与增殖
适用于(设备)荧光显微镜
产品线Newport Green
产品类型DCF 二乙酸酯
Unit SizeEach
内容与储存
在冰箱中(-5°C 至 -30°C)储存。

引用和文献 (48)

引用和文献
Abstract
Calcineurin/nuclear factor of activated T cells and MAPK signaling induce TNF-{alpha} gene expression in pancreatic islet endocrine cells.
Authors:Lawrence MC, Naziruddin B, Levy MF, Jackson A, McGlynn K,
Journal:J Biol Chem
PubMed ID:21059644
Cytokines contribute to pancreatic islet inflammation, leading to impaired glucose homeostasis and diabetic diseases. A plethora of data shows that proinflammatory cytokines are produced in pancreatic islets by infiltrating mononuclear immune cells. Here, we show that pancreatic islet a cells and ß cells express tumor necrosis factor-a (TNF-a) and other ... More
Identification and purification of functional human beta-cells by a new specific zinc-fluorescent probe.
Authors:Lukowiak B, Vandewalle B, Riachy R, Kerr-Conte J, Gmyr V, Belaich S, Lefebvre J, Pattou F
Journal:J Histochem Cytochem
PubMed ID:11259455
'Pancreatic beta-cells contain large amounts of zinc. We took advantage of this to try to localize, quantify, and isolate insulin-producing cells from islet preparations. Our study was designed to identify a non-toxic zinc-sensitive fluorescent probe able to selectively label labile zinc in viable beta-cells and to exhibit excitation and emission ... More
Preferential Zn2+ influx through Ca2+-permeable AMPA/kainate channels triggers prolonged mitochondrial superoxide production.
Authors:Sensi SL, Yin HZ, Carriedo SG, Rao SS, Weiss JH
Journal:Proc Natl Acad Sci U S A
PubMed ID:10051656
'Synaptically released Zn2+ can enter and cause injury to postsynaptic neurons. Microfluorimetric studies using the Zn2+-sensitive probe, Newport green, examined levels of [Zn2+]i attained in cultured cortical neurons on exposure to N-methyl-D-asparte, kainate, or high K+ (to activate voltage-sensitive Ca2+ channels) in the presence of 300 microM Zn2+. Indicating particularly ... More
Measurement of intracellular free zinc in living cortical neurons: routes of entry.
Authors:Sensi SL, Canzoniero LM, Yu SP, Ying HS, Koh JY, Kerchner GA, Choi DW
Journal:J Neurosci
PubMed ID:9391010
'We used the ratioable fluorescent dye mag-fura-5 to measure intracellular free Zn2+ ([Zn2+]i) in cultured neocortical neurons exposed to neurotoxic concentrations of Zn2+ in concert with depolarization or glutamate receptor activation and identified four routes of Zn2+ entry. Neurons exposed to extracellular Zn2+ plus high K+ responded with a peak ... More
Protection by glycine against hypoxic injury of rat hepatocytes: inhibition of ion fluxes through nonspecific leaks.
Authors:Frank A, Rauen U, de Groot H
Journal:J Hepatol
PubMed ID:10673068
'BACKGROUND/AIMS: Glycine has long been shown to exert strong protective effects against hypoxic injury of hepatocytes. Recently, it was suggested that glycine exerts this protection via inhibition of ligand-gated chloride channels, thereby secondarily inhibiting sodium influx. The purpose of this study was to examine this suggestion. METHODS: Cultured rat hepatocytes ... More